Activation of bovine oocytes by strontium combined or not with an electric pulse

Leal, Cláudia Lima Verde; Méo, Simone Cristina; García, J. M.

doi:10.1590/s0102-09352003000300020

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…Although the resumption of meiotic division could be induced by a single calcium rise, higher embryonic development was observed when multiple rises in calcium levels were promoted by strontium to induce artificial activation of reconstructed oocytes in mice (Kishikawa et al, 1999). In cattle, previous results from our laboratory have shown that strontium is able to induce activation (Leal et al, 2003) and parthenogenetic development to the blastocyst stage (Méo et al, 2004(Méo et al, , 2005 in cattle, suggesting that it may be possible to use this activating agent as an alternative method for activation procedures used for cloning by NT in this species.…”

Section: Discussionmentioning

confidence: 89%

“…It has been reported that strontium is able to induce calcium oscillations in mouse oocytes in a similar pattern to that caused by spermatozoa during fertilization (Bos-Mikich et al, 1997), and repeated calcium rises seem to be beneficial for later embryo development (Vitullo & Ozil, 1992). Previous experiments from our laboratory have shown that strontium can also be used for the activation of bovine oocytes (Leal et al, 2003;Méo et al, 2004a, b). However, there are no reports on its application in bovine cloning.…”

Section: Introductionmentioning

confidence: 86%

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Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer

Yamazaki

Ferreira

Méo

et al. 2005

Zygote

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SummaryAs an important step in the nuclear transfer (NT) procedure, we evaluated the effect of three different treatments for oocyte activation on the in vitro and in vivo developmental capacity of bovine reconstructed embryos: (1) strontium, which has been successfully used in mice but not yet tested in cattle; (2) ionomycin and 6-dimethylaminopurine (6-DMAP), a standard treatment used in cattle; (3) ionomycin and strontium, in place of 6-DMAP. As regards NT blastocyst development, no difference was observed when strontium (20.1%) or ionomycin/6-DMAP (14.4%) were used. However, when 6-DMAP was substituted by strontium (3), the blastocyst rate (34.8%) was superior to that in the other activation groups (p < 0.05). Results of in vivo development showed the possibility of pregnancies when NT embryos activated in strontium were transferred to recipient cows (16.6%). A live female calf was obtained when ionomycin/strontium were used, but it died 30 days after birth. Our findings show that strontium can be used as an activation agent in bovine cloning procedures and that activation with a combination of strontium and ionomycin increased the in vitro developmental capacity of reconstructed embryos. This is the first report of a calf produced by adult somatic cell NT in Latin America.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 86%

Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer

Yamazaki

Ferreira

Méo

et al. 2005

Zygote

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“…Many different treatments have already been used in an attempt to mimic the activation induced by the spermatozoon, including physical methods such as electrical pulse (Ozil, 1990) or chemical methods, such as ethanol (Liu et al, 1998), calcium ionophore (Soloy et al, 1997), ionomycin (Susko-Parrish et al, 1994), strontium chloride (Wakayama et al, 1998;Méo et al, 2002;Leal et al, 2003), cycloheximide (Presicce and Yang, 1994;Liu et al, 1998), or 6-dimethylaminopurine (6-DMAP) (Susko-Parrish et al, 1994;Loi et al, 1998). These activation treatments can be used either alone or in combination.…”

Section: Introductionmentioning

confidence: 99%

Oocyte activation and preimplantation development of bovine embryos obtained by specific inhibition of cyclin-dependent kinases

Perecin

Méo

Leal

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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The efficiency of bohemine and roscovitine in combination with ionomycin on parthenogenetic activation and initial embryonic development of bovine oocytes was studied. Two experiments were performed: in the first, different concentrations (0, 50, 75 or 100µM) and different exposure periods (2, 4 or 6 hours) to bohemine or roscovitine were tested for activation rates of in vitro matured (IVM) bovine oocytes, which were pre-exposed to ionomycin. The best treatments, 75µM bohemine and 50µM roscovitine, both for 6h, were used in the second experiment, in which IVM bovine oocytes were exposed to ionomycin, followed or not by bohemine or roscovitine treatment, and evaluated for nuclear status, activation rate and blastocyst development were assessed. The combined treatments (ionomycin + cyclin-dependent kinases inhibitors -CDKIs) showed better results for activation rates (77.3%) and initial embryonic development (35.2%) than the single ionomycin treatment (69.4% for activation and 21.9% for development); and also lead to a more uniform activation (nearly 90% single pronucleus development). The results showed that CDKIs improve the effects of ionomycin on parthenogenetic activation and blastocyst development in bovine oocytes and could help to achieve more efficient activation protocols, increasing the developmental competence of embryos obtained by reproductive biotechniques.Keywords: bovine, artificial activation, bohemine, roscovitine, parthenogenesis RESUMO Realizaram-se dois experimentos para avaliar a eficiência da bohemina e roscovitina associadas à ionomicina para ativação partenogenética e desenvolvimento embrionário inicial de bovinos. No primeiro, foram testadas diferentes concentrações (0, 50, 75 ou 100µM) e diferentes tempos de exposição (2, 4 ou 6 horas) à bohemina ou à roscovitina na ativação de oócitos bovinos maturados in vitro (MIV) pré-expostos à ionomicina. Os melhores tratamentos, bohemina 75µM e roscovitina 50µM, ambos por seis horas, foram utilizados no segundo experimento, no qual oócitos bovinos MIV foram expostos à ionomicina seguido ou não pelo tratamento com inibidores específicos das quinases dependentes de ciclina (CDKI), e avaliados quanto à configuração nuclear

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“…Enquanto a ionomicina promove um único pico de Ca 2+ no período inicial da ativação in vitro, sabe-se que a ação do Sr 2+ em oócitos de camundongos é muito similar à do espermatozóide, pela seqüência de picos desencadeada. Em bovinos, o Sr 2+ associado ou não a pulso elétrico já foi utilizado com bons resultados (MÉO, 2003), este foi o primeiro relato da eficácia dessa substância para tal finalidade no entanto, seu mecanismo de ação ainda não foi demonstrado.…”

Section: Agentes Químicos Ativadoresunclassified

Ativação partenogenética de oócitos bovinos jovens com ionomicina e 6-dimetilaminopurina associado ou não ao estrôncio

Porciúncula¹

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Activation of bovine oocytes by strontium combined or not with an electric pulse

Cited by 5 publications

References 16 publications

Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer

Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer

Oocyte activation and preimplantation development of bovine embryos obtained by specific inhibition of cyclin-dependent kinases

Ativação partenogenética de oócitos bovinos jovens com ionomicina e 6-dimetilaminopurina associado ou não ao estrôncio

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