Infecção experimental de cães com amostras de Babesia canis isoladas em Minas Gerais

Bicalho, Kelly Alves; Passos, Lygia Maria Friche; Ribeiro, Múcio Flávio Barbosa

doi:10.1590/s0102-09352002000500016

Cited by 5 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunofluorescence Assay (IFA) using antigens of B. canis vogeli obtained from a splenectomized dog that was experimentally infected with isolate from Belo Horizonte-Minas Gerais (Bicalho et al 2002). The antigen was produced following the methodology described by IICA (1984) with modifications.…”

Section: Methodsmentioning

confidence: 99%

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

et al. 2015

View full text Add to dashboard Cite

Abstract:This study aimed to report the prevalence of Babesia canis vogeli in dogs and ticks in the urban and rural areas of Petrolina, Pernambuco. Serum and peripheral blood samples of 404 dogs were tested by indirect immunofluorescence assay (IFA) and by blood smears, respectively. The presence of tick infestation was evaluated, and some specimens were submitted to DNA amplification by polymerase chain reaction (PCR). The presence of antibodies anti-B. canis vogeli was determinate in 57.9% (234/404) of dogs. The direct detection of Babesia spp was obtained in 0.5% (2/404) dogs by visualization of intraerythrocytic forms. Infestation by Rhipicephalus sanguineus sensu lato was observed in 54.5% (220/404) of dogs in both urban and rural areas. DNA of Babesia canis vogeli were obtained by PCR in 6% individual (3/50) and 8.7% of pool of ticks (7/80). The risk factors for the presence of anti-B. canis vogeli antibodies, as determined through the application of logistic regression models (P<0.05), were the following: medium breed size variables (P<0.001); contact with areas of forest (P=0.021); and access on the street (P=0.046). This study describes, for the first time, the confirmation of infection of B. canis vogeli in dogs and ticks in the semiarid region of Pernambuco, Brazil.

show abstract

Section: Methodsmentioning

confidence: 99%