Ocorrência de tuberculose em rebanhos bubalinos (Bubalus bubalis var. bubalis-Linneus, 1758) no Município de Parintins, Amazonas

Mota, Pedro; Lobato, Francisco Carlos Faria; Assis, Ronnie Antunes de; Lage, Andrey Pereira; Parreiras, Patrícia Martins; Leite, Rômulo Cerqueira

doi:10.1590/s0102-09352002000400017

Cited by 9 publications

(8 citation statements)

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“…The prevalence in buffaloes seems to agree with previous studies in the region (Barbosa et al, ; Mota et al, ); however, in the first study, out of 266 skin test reagent animals, only 14 were sacrificed for microbiological analysis and in our second study, only TST test was performed and all TST reagent animals were tested for microbiological and molecular diagnosis of M. bovis . In our study, the higher prevalence in buffalos might be explained by three factors: an environmental factor—in this study, buffalo herds had less herd health than cattle herds; a behavioural factor—buffaloes under pasture have a high tendency to stay closer to each other than cattle which favours the transmission of the M. bovis , or a genetic factor, buffalo can be more susceptive to specific M. bovis strain.…”

Section: Resultssupporting

confidence: 91%

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Carneiro

Pasquatti

Takatani³

et al. 2019

Veterinary Medicine & Sci

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The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.

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Section: Resultssupporting

confidence: 91%

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Carneiro

Pasquatti

Takatani³

et al. 2019

Veterinary Medicine & Sci

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“…The sample size should be determined based on expected prevalence in samples from slaughterhouses, however, we are unable to find a previous study with this sample source in the region. In Amazonas state, only one study about prevalence of BTB in buffalos (Bubalus bubalis) was found, based on comparative cervical test (CCT) showing a prevalence of 20.4% (38). Recent studies about BTB prevalence in the region, also based on CCT, showed results ranging from 0.12 (cattle) to 7.2% (buffalos) in Rondonia and Para State, respectively (24,39).…”

Section: Sample Sizementioning

confidence: 99%

Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in Amazonas, Brazil

Carneiro

Takatani²,

Pasquatti

et al. 2019

Front. Vet. Sci.

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Bovine Tuberculosis (BTB) is an endemic disease in about one hundred countries, affecting the economy causing a decrease in productivity, condemnation of meat, and damaging the credibility on international trade. Additionally, Mycobacterium bovis the major causative agent for BTB can also infect humans causing a variety of clinical presentations. The aim of this study was to determine BTB prevalence and the main risk factors for the Mycobacterium bovis prevalence in cattle and buffalos in Amazonas State, Brazil. Tissue samples from 151 animals (45 buffalo and 106 cattle from five herds with buffalo only, 22 herds with cattle only, and 12 herds with buffalo and cattle) were obtained from slaughterhouses under State Veterinary Inspection. M. bovis were isolated on Stonebrink medium. The positive cultures were confirmed by polymerase chain reaction (PCR) testing. The apparent herd and animal prevalence rates were 56.4 and 5.40%, respectively. Regarding animal species, the apparent prevalence rates were 3% in cattle and 11.8% in buffalo. Generalized Linear Mixed Models (GLMM) with random effect were used to assess the association with risk factors on the prevalence. Species (buffalo), herds size (>100 animals) and the presence of both species (buffalo and cattle) in the herd were the major risk factors for the infection by Mycobacterium bovis in the region. The findings reveal an urgent need for evidence-based effective intervention to reduce BTB prevalence in cattle and buffalo and prevent its spread to the human population. Studies are needed to understand why buffalo are more likely to be infected by M. bovis than cattle in Amazon. Recommendations for zoning, use of data from the inspection services to generate information regarding BTB focus, adoption of epidemiological tools, and discouragement of practices that promote the mixing of cattle and buffalo, were made.

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“…Despite the identification of tuberculosis outbreaks in buffaloes of Pakistan, the UK, India, Thailand, Nepal, South Africa, Argentina, and Brazil, the disease has hardly been studied in these animals (GARINE-WICHATITSKY et al, 2010;KANAMEDA et al, 1999;LOPES et al, 2006;MICHEL, 2007MICHEL, , 2008MOTA et al, 2002;O'REILLY;ROSÁRIO, 2010). Currently, literature discusses lesions that are commonly found and ways of diagnosing tuberculosis in buffalo.…”

Section: Diagnosismentioning

confidence: 99%

“…In 2011, Ribeiro reported that 0.93% of the carcasses at a slaughterhouse in the metropolitan region of Belém, PA, resulted from slaughter due to hepatic tuberculosis lesions. Mota et al (2002) followed the slaughter of 14 animals in the State of Amazonas that were considered suspect following tuberculin tests, and they found alterations, mainly in the respiratory lymph nodes. The data collected by Ribeiro (2011) corroborated that presented by Freitas et al (2001), and both authors attribute the occurrence of buffalo tuberculosis in the Marajó Island to the high rainfall in this location and the gregarious habits of the buffaloes, which permits airborne horizontal contamination.…”

Section: Diagnosismentioning

confidence: 99%

Tuberculosis diagnostic methods in buffaloes

et al. 2015

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The low productivity of buffalo herds and condemnation of carcasses in slaughterhouses due to tuberculosis lesions have resulted in increasing economic losses because these animals cannot be treated and must be destroyed by sanitary slaughter. Tuberculosis is a widely distributed zoonosis that affects the beef supply chain of the Brazilian agribusiness economically and socially. Like cattle, buffaloes are sensitive to Mycobacterium bovis, which is the main causative agent of zoonotic tuberculosis. Tuberculosis in buffaloes has been reported in several countries, including Brazil. In order to control and eradicate this disease among cattle and buffaloes in Brazil, the Ministry of Agriculture, Livestock, and Supply created the National Program for the Control and Eradication of Brucellosis and Tuberculosis with the main objective of finding a significant number of disease-free herds throughout the national territory using reliable methods. This review summarizes the main data on the history of occurrence of M. bovis in Brazilian herds and the diagnostic methods for the disease in buffaloes. Little information is available on buffalo tuberculosis. Due to the increasing population of buffaloes and their economic importance, more studies investigating the occurrence and identification of tuberculosis in this species are clearly needed. Key words: Mycobacterium bovis, isolation, zoonosis, bovine ResumoSão crescentes as perdas econômicas com baixa na produtividade dos rebanhos bubalinos e a condenação de carcaças em matadouros devido à tuberculose, uma vez que os animais não podem ser tratados e devem ser descartados em abates sanitários. A doença tem impacto econômico e social para a cadeia produtiva do agronegócio brasileiro, além de ser uma zoonose de ampla distribuição. Assim como os bovinos, o búfalo é sensível ao Mycobacterium bovis, principal agente da tuberculose zoonótica. A ocorrência da tuberculose nessa espécie tem sido relatada em diversos países, inclusive no Brasil. Para controle e erradicação dessa enfermidade entre os bovinos e bubalinos no Brasil, o Ministério da Agricultura Pecuária e Abastecimento (MAPA) instituiu o Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose (PNCEBT), cujo principal objetivo é, por meio de métodos confiáveis, obter significativo número de rebanhos livres dessas doenças, em todo o território nacional. Esta revisão reuniu os principais dados referentes ao histórico da ocorrência de M. bovis nos rebanhos do Brasil e aos métodos diagnósticos da doença em búfalos. Na literatura faltam dados relativos à tuberculose bubalina e com o incremento da criação desses animais e de sua importância econômica, notou-se evidente necessidade de estudos e pesquisas relativos à ocorrência e identificação da tuberculose nessa espécie. Palavras-chave: Mycobacterium bovis, isolamento, zoonose, bovídeos

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Ocorrência de tuberculose em rebanhos bubalinos (Bubalus bubalis var. bubalis-Linneus, 1758) no Município de Parintins, Amazonas

Cited by 9 publications

References 0 publications

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in Amazonas, Brazil

Tuberculosis diagnostic methods in buffaloes

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