Histopathological and immunohistochemical study of the gastrointestinal tract from a dog naturally infected with Leishmania (Leishmania) chagasi: a case report

Silva, F.L.; Oliveira, Márcia Rosa de; Tafuri, Washington Luiz

doi:10.1590/s0102-09352002000400002

Cited by 11 publications

(17 citation statements)

References 10 publications

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“…We observed parasites associated with the inflammatory response throughout the mucosa, but without inducing severe pathologies, such as ulcers or necrosis. The immunohistochemical morphometric analysis revealed higher parasite loads in the colon than in the jejunum, as previ-ously reported by our group (25) and in accordance with other reports (31)(32)(33)(34)(35)(36).…”

Section: Discussionsupporting

confidence: 92%

Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum

et al. 2014

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Section: Discussionsupporting

confidence: 92%

Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum

et al. 2014

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“…Despite the parasite burden throughout the GIT mucosa, no ulcer, focus of hemorrhage, or marked pathological alterations were observed. These results are in accordance with Silva et al [7] and Pinto et al [1]. However, Adamama-Moraitou et al [8], using colonoscopy, and immunohistochemistry observed mild erosive colonic mucosa in eight of 31(25.8%) symptomatic dogs naturally infected with L. infantum .…”

Section: Discussionsupporting

confidence: 90%

Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum

et al. 2013

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BackgroundInfection with parasite protozoa is a long-term health issue in tropical and subtropical regions throughout the world. The Toll-like receptor (TLR) signaling pathway is one of the first-responding defense systems against Leishmania. The aim of this study was to investigate the expression of TLR2 and TLR9 in jejunum and colon and its correlation with CD11c, CD11b, and CD14 receptors used as markers for dendritic cells and macrophages.MethodsTwenty four dogs infected with Leishmania infantum were used in this study. Cytometry was carried out in lamina propria cells from jejunum and colon using markers for TLR2, TLR9, CD11b, CD11c and CD14.ResultsCellular inflammatory exudate was diffuse in the mucosa and submucosa, predominately comprising mononuclear cells: plasma cells, macrophages, and lymphocytes. Despite the parasite load, microscopy showed no erosion was evident in the epithelial mucosa layers. The colon harbored more parasites than the jejunum. Flow cytometry revealed higher frequency of TLR2+ and CD11c+ dendritic cells in the colon than in the jejunum. Conversely, TLR9-expressing cells were more frequent in jejunum. Moreover, frequency of macrophages (CD11b+ and CD14+) expressing simultaneity TLR9 were lower in the colon than in jejunum, while CD11c+ cells predominated in the colon. Despite of the negative ELISA serum results, IL-10 and TNF-α were higher in jejunum than colon of infected animals. However, IL-4 was higher in colon than jejunum of infected animals. A higher expression these cytokines were demonstrated in infected dogs compared to uninfected dogs.ConclusionsThere was no correlation between clinical signs and pathological changes and immunological and parasitological findings in the gastrointestinal tract in canine visceral leishmaniasis. However, jejunum showed a lower parasite load with increased frequency and expression of CD11b, TLR9, CD14/CD11b/TLR9 receptors and IL-10 and TNF-α cytokines. Conversely, the colon showed a higher parasite load along with increased frequency and expression of TLR2, CD11c receptors, and IL-4 cytokine. Thus, Leishmania infantum is able to interfere in jejunum increased expression of TLR2, TLR9, CD11b, CD14, CD14/CD11b/TLR9 receptors, IL-10, and TNF-α; and in colon increased expression of CD11c, TLR2, TLR9, CD11b, CD14 e, CD14/CD11b/TLR9 receptors, IL-10, and TNF-α.

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“…In the same dog, denudation of the epithelium and lymphangiomatous polyps were observed at the tips of the villi of the duodenum in association with severe inflammatory mononuclear infiltrate and high parasite burden. This association between lymphangiomatous polyps and intestinal leishmaniasis was a novel feature of the present study, although Silva et al (2002) reported polyp like formation in the intestinal mucosa of dogs affected by CVL.…”

Section: Discussionsupporting

confidence: 56%

“…More recently, Silva et al (2002) and Pinto et al (2011) investigated symptomatic dogs that were naturally infected with L. infantum and observed high parasite burden throughout the intestinal mucosa (predominantly in the colon) without marked pathological alterations, which suggested that Leishmania might be a cause of intestinal immunological tolerance.…”

Section: Introductionmentioning

confidence: 99%

Correlation study and histopathological description of intestinal alterations in dogs infected with Leishmania infantum

Silva

Neves

Queiroz

et al. 2016

Rev. Bras. Parasitol. Vet.

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The aim of this work was a correlation study and histopathological description of alterations associated with the presence of Leishmania infantum amastigote in the intestinal wall of dogs infected with canine visceral leishmaniasis (CVL). Three groups were used: G1 (n = 8), comprising naturally infected dogs with CVL with amastigotes of L. infantum in the small and large intestines; G2 (n = 9), infected dogs with CVL, without intestinal amastigotes; and G3 (n = 3), uninfected dogs. Histochemistry and immunohistochemistry methods were used for histopathology and amastigotes identification. 47.1% (8/17) of dogs from G1 group had amastigotes in the mucosa, submucosa and muscle layers of the small and large intestines and it was observed a prominent inflammatory reaction characterized by chronic infiltration of mononuclear cells: macrophages, lymphocytes and plasma cells. Comparison between the groups showed only a significant difference in relation to mucosal microscopic structural alterations in dogs from G1 in relation to G2 and G3. Parasite burden showed significant correlations with the microscopic alterations and clinical status of dogs in G1. By the conclusion, the inflammatory reactions caused by the parasites in the intestines might have contributed towards alterations in digestive processes, worsening the dogs' clinical status of CVL.Keywords: Dog, histology, immunohistochemistry, intestine, leishmaniasis, amastigotes. ResumoO objetivo foi realizar um estudo de correlação e descrição histopatológica das lesões associadas à presença de amastigotas de Leishmania infantum na parede intestinal de cães infectados com leishmaniose visceral canina (LVC). Os cães foram subdivididos em três grupos: G1 (n = 8) cães naturalmente infectados com LVC e com amastigotas de L. infantum no intestino; G2 (n = 9) com LVC, mas sem o parasitismo intestinal; e G3 (n = 3) cães não infectados. Métodos histoquímicos e imunoistoquímicos foram utilizados para a histopatologia e a identificação das amastigotas, respectivamente. 47,1% (8/17) dos cães infectados (grupo G1) apresentavam formas amastigotas na mucosa, submucosa e camada muscular do intestino delgado e grosso, destacando-se uma reação inflamatória caracterizada por infiltrado crônico de células mononucleares; macrófagos, linfócitos e plasmócitos. Observou-se uma diferença significativa somente com relação às alterações estruturais microscópicas intestinais nos cães do G1 quando comparadas com G2 e G3. A intensidade parasitária intestinal teve correlação significativa com as alterações microscópicas e os sinais clínicos dos cães do G1. Concluiu-se que as amastigotas de L. infantum por causarem reações inflamatórias na parede intestinal dos cães podem ter contribuído para as alterações dos processos digestórios, agravando ainda mais o quadro clínico dos animais.Palavras-chave: Cão, histologia, imunoistoquímica, intestino, leishmaniose, amastigotas.

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Histopathological and immunohistochemical study of the gastrointestinal tract from a dog naturally infected with Leishmania (Leishmania) chagasi: a case report

Cited by 11 publications

References 10 publications

Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum

Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum

Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum

Correlation study and histopathological description of intestinal alterations in dogs infected with Leishmania infantum

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