Resultados preliminares da utilização de cultivos de anéis de traquéia para o estudo de estirpes brasileiras do vírus da bronquite infecciosa das galinhas

Epiphanio, E.O.B.; Martins, Nélson Rodrigo da Silva; Resende, J.S.; Pinto, Rosário; Jorge, M. H. A.; Souza, Menira; Caccioppoli, J.; Cardozo, Rejane Machado

doi:10.1590/s0102-09352002000200013

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…Tracheal rings were evaluated for ciliary motility at 24, 48, 72, and 96 h post-inoculation. Ciliary motility decreases as IBV replicates in the tracheal cells (Epiphanio et al 2002). This technique also has disadvantage of not being sensitive for IBV field samples and may result in false-negative results.…”

Section: Cultivation In Tracheal Ringsmentioning

confidence: 99%

Avian Infectious Bronchitis Virus

Ramakrishnan

Kappala

2019

Recent Advances in Animal Virology

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Avian infectious bronchitis (IB) is caused by avian infectious bronchitis virus (IBV) belonging to Coronaviridae family. The disease is prevalent in all countries with almost 100% incidence rate. Chicken and commercially reared pheasant are the natural host for IBV. Virus causes respiratory diseases, poor weight gain, feed efficiency in broiler, damage to oviduct, and abnormal egg production in mature hens resulting in economic losses. IBV also replicates in tracheal and renal epithelial cells leading to prominent tracheal and kidney lesions. Virus undergoes spontaneous mutation leading to continual emergence of new variants. The effectiveness of immunization program is diminished because of poor cross-protection among the serotypes. Identification of circulating serotypes is important in controlling IBV infection. Toll-like receptor 3 (TLR3) and TLR21 are involved in early recognition of virus resulting in induction of inflammatory cytokines. Both humoral and cellular immune responses are important in the control of infection. Humoral immunity plays an important role in recovery and clearance of viral infection. IBV-specific cytotoxic T lymphocytes induce lysis of IBV-infected cells. Effective diagnostic tools are required at field level to identify different IBV variants. Embryonated chicken eggs are effective model for virus isolation. Identification by other specific methods like virus neutralization (VN), hemagglutination inhibition (HI), enzyme linked immunosorbent assay (ELISA), immunohistochemistry, or nucleic acid analysis or by electron microscopy is also indispensable. VN test in tracheal organ culture is the best method for antigenic typing for surveillance purposes. Continuous epidemiological surveillance, strict biosecurity measures, and vaccine effective against various serotypes are necessary for controlling IB in chickens.

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Section: Cultivation In Tracheal Ringsmentioning

confidence: 99%

Avian Infectious Bronchitis Virus

Ramakrishnan

Kappala

2019

Recent Advances in Animal Virology

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“…At 24, 48, 72 and 96 hours after inoculation, the trachea rings are observed in inverted optical microscope, evaluating the ciliary motility, which should decrease due to replication of IBV (Epiphanio et al, 2002).…”

Section: Cultivation In Trachea Ringsmentioning

confidence: 99%

Diagnosis of infectious bronchitis: an overview of concepts and tools

Villarreal

2010

Rev. Bras. Cienc. Avic.

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Infectious bronchitis (IB) casues multi-systemic infection in chickens with signs similar caused by other poultry pathogens and thus a high diagnostic accuracy can only be achieved by s series of laboratory assays. This article reviews in a brief way the traditional virus assays such as embryo innoculation, tracheal rings and virus neutralization assays for the direct detection of Avian infectious bronchitis virus (IBV) and methods based on gene molecular biology and some assays for the detection of anti-IBV antibodies, including ELISA.A critical view on each technique is also provived by the author.

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“…Cultivos de anéis de traqueia foram usados para estudos de cepas de IBV e da resposta imune em galinhas experimentalmente e naturalmente infectadas (EPIPHANIO et al, 2002). O estudo em cultivos de anéis de embriões SPF é o método laboratorial que melhor se aproxima das condições in vivo, embora seja técnica trabalhosa e não oferecida pelos laboratórios em rotina.…”

Section: Diagnósticounclassified