Apoptose no tumor venéreo transmissível canino: características morfológicas e evidenciação bioquímica

Santos, F.G.A.; Vasconcelos, A.C.; Moro, Luciana; Nunes, J.

doi:10.1590/s0102-09352001000500008

Cited by 6 publications

(6 citation statements)

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“…The histopathological aspects observed for the naturally acquired and the experimentally transplanted TVTs were in agreement with the (Stewart et al, 1959) and were similar to results of a previous paper on naturally acquired TVT (Santos et al, 2001). The composition and quantity of the stroma observed in the regressive transplanted tumors were more evident than in the growing transplanted TVTs, similarly to genital TVTs (Stewart et al, 1959).…”

Section: Discussionsupporting

confidence: 89%

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Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Santos¹,

Vasconcelos²,

Nunes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in the regressive tumors. It was also confirmed by transmission electron microscopy, which showed cells with typical morphology of apoptosis and by the TUNEL reaction that detected in situ the 3'OH nick end labeling mainly in the regressive tumors. The regressive TVTs also showed an intensified immunostaining for P53 besides a more intense genomic DNA fragmentation detected by the agarose gel electrophoresis. In conclusion, apoptosis has an important role in the regression of the experimental TVT in a way that is P53-dependent.Keywords: dog, venereal tumor, apoptosis, cell death RESUMO Doze cães, adultos, machos e sem raça definida foram transplantados subcutaneamente, na região hipogástrica, com células originadas de dois tumores venéreos transmissíveis caninos (TVT). O objetivo do estudo foi demonstrar e quantificar a ocorrência de apoptose na regressão do TVT. Após seis meses

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Section: Discussionsupporting

confidence: 89%

“…The TVT frequently regresses spontaneously in healthy, adult animals. The regression is associated with lymphocytes and plasma cells infiltration as well as necrosis (Cohen, 1978;Pérez et al, 1998) and apoptosis (Santos et al, 2001). Fibrosis may occur during the final stages of the regression (Stewart et al, 1959).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Santos¹,

Vasconcelos²,

Nunes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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“…The canine transmissible venereal tumor (CTVT) is a peculiar neoplasm resulting from the transmission of cancerous cells of the host to another canid by implantation process [34]. Transmission occurs among reproductive age dogs, especially those with unrestricted sexual activity and access to the streets, by cell implantation during coitus, licking or interaction between the infected animal and the susceptible host [7,29,31]. It usually occurs on the external genitalia of male and female dogs and cases not affecting the genital area are unusual.…”

Section: Introductionmentioning

confidence: 99%

Primary Ocular Transmissible Venereal Tumor in a Prepubescent Female Dog

Amaral

Mucha

Oliveira

et al. 2020

Acta Scientiae. Vet.

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Background: Canine transmissible venereal tumor (CTVT) is a peculiar neoplasm resulting from the transmission of host cancerous cells to another canid by implantation. Transmission occurs among reproductive age dogs, especially those with unrestricted sexual activity. It usually occurs on the external genitalia of dogs and other areas are unusual. However, implants have been described in injured mucosa, as well as metastases in lymph nodes, spleen, skin, anus and perianal space, oral mucosa, nasal mucosa, eyeball and brain. The purpose of this report is to describe the first case of exclusively extragenital ocular CTVT in a prepubescent female dog.Case: A 6-month-old mixed-breed, non-spayed and prepubescent female dog, showing a fast-growing reddish-colored mass in the right eye (RE), was examined. Blepharospasm, mild serosanguineous secretion, chemosis and a smooth surface reddish mass with 2.5 cm in length occupying the orbital area were observed during physical examination, impairing the visualization of the right eyeball. For ocular ultrasonography examination of the affected eye, acepromazine was used (0.03 mg/kg) associated with methadone (0.3 mg/kg) intramuscularly, and propofol (4 mg/kg) for induction and 0.2 mg/kg for maintenance, intravenously. Ultrasonography examination evidenced an amorphous hyperechogenic structure, medial to the RE, with homogeneous echotexture, punctiform vascularization to amplitude Doppler (Power Doppler), measuring 2.8 cm in length and 1.4 cm in diameter, causing eyeball distortion and rejection. Based on ultrasonography results and with the patient still anesthetized, an incisional biopsy of the peribulbar mass was performed with a 5 mm disposable punch. The final CTVT diagnosis was possible after histopathological analysis. No more CTVT nodules were found, especially in the vulva, which had a prepubescent appearance, consistent with age. Treatment with 4 sessions of chemotherapy with vincristine sulfate resulted in complete remission of the tumor mass. In the second session, it was already possible to visualize the eyeball with a significant remission of the tumor mass, ruling out neoplastic exceresis. At this time, threat response, normal direct and consensual photopupillary reflexes, normal eyelid reflex, corneal surface without slit lamp biomicroscopy and fluorescein staining were present in the RE. Nevertheless, there were mild diffuse corneal edema and slight ciliary injection. One percent prednisolone eye drops (8/8 h) and tobramycin eye drops (6/6 h) were prescribed for 1 week. In the fourth session of chemotherapy, no changes were observed in the RE.Discussion: In the present case, the diagnosis of CPVD in a prepubertal patient, primary orbital TVTV, and the fact that the structure of the eye bulb and visual function remain intact, even with the significant volume of orbital neoplasia, are highlighted. A single report of skin CTVT in an 11-month-old prepubescent female dog was found. It is important to emphasize that CTVT is typically of dogs of reproductive age that have genital masses. Usually, extragenital masses are related to the act of licking, scratching, biting or sniffing the primary tumor (self-implantation), or rarely, by metastasis, a fact not observed in the present report, since the ocular mass was the only manifestation. Treatment was conducted according to indications of the literature. Four injections of vincristine sulfate were necessary, observing complete remission of the tumor mass with no relapse after 30 days. In this case report, it was possible to observe that CTVT may reveal unusual behaviors regarding epidemiology, which, added to the rare form of exclusively extragenital ocular clinical manifestation, impose a diagnostic challenge, making the histopathological examination an important tool for diagnosis.

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“…A transmissão deste se dá pela cópula, podendo ser transplantado mecanicamente por meio de interações entre animais infectados e sadios, tais como lambedura, mordedura, arranhões (FILGUEIRA, 2010) ou até mesmo por transplantes experimentais (SANTOS et al, 2001). O atrito associado ao coito em cães pode resultar em lesões genitais, tornando-os susceptíveis à transplantação de células do tumor, onde a mucosa lesada (escoriações ou soluções de continuidade) se torna a porta de entrada da implantação das células tumorais, bem como o contato prolongado de mucosas íntegras (MILO & SNEAD, 2014).…”

Section: Introductionunclassified

Tumor Venéreo Transmissível Primário Em Terceira Pálpebra Com Migração Para Cavidade Nasal – Relato De Caso

Maciel¹,

Schroder²,

Cruz³

et al. 2015

Enciclopédia Biosfera

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RESUMO O tumor venéreo transmissível (TVT) é uma neoplasia contagiosa entre cães sexualmente maduros, transmitido pela cópula. É normalmente encontrado na mucosa genital, porém já foi relatado em sítios extragenitais, sendo que raramente faz metástase para outros órgãos do corpo. Relata-se o caso de uma cadela de oito anos, sem raça definida, atendida no Hospital Veterinário da Universidade Federal de Mato Grosso que apresentava nodulações com aproximadamente 0,5 cm de diâmetro na face anterior da terceira pálpebra do olho direito, de coloração avermelhada e secreção seromucosa, com migração para cavidade nasal, ocasionando sinais clínicos como secreção nasal e dificuldade respiratória. Através da citologia, observaram-se agregados de células arredondadas com núcleo hipercorado e citoplasma basofílico, sendo o diagnóstico estabelecido como TVT. O animal foi submetido ao tratamento quimioterápico a base de sulfato vincristina na dose de 0,05 mg/kg, a cada sete dias, por via intravenosa. Embora a apresentação do caso seja atípica, a resposta à quimioterapia foi excelente, levando a remissão completa da neoplasia após a sétima semana de tratamento.

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Apoptose no tumor venéreo transmissível canino: características morfológicas e evidenciação bioquímica

Cited by 6 publications

References 6 publications

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Primary Ocular Transmissible Venereal Tumor in a Prepubescent Female Dog

Tumor Venéreo Transmissível Primário Em Terceira Pálpebra Com Migração Para Cavidade Nasal – Relato De Caso

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