Occurrence of Campylobacter in the genitals of teaser bulls maintained at an embryo transfer center

Modolo, José Rafael; Lopes, C. A.; Genari, T.

doi:10.1590/s0102-09352000000200002

Cited by 6 publications

(5 citation statements)

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“…For example, the discovery of Campylobacter fetus subsp. venerealis in the metagenomics study investigating preputial samples from healthy bulls reinforced the previous findings regarding the role of bulls in spreading venereal diseases while remaining asymptomatic [ 17 , 18 , 20 , 60 ]. Meanwhile, metagenomics studies also revealed that the lower reproductive tract of cows has a different microbial composition compared to primates and humans, which both have Lactobacillus spp.…”

Section: Discussionsupporting

confidence: 80%

“…However, in some cases, bacteria can persistently colonize the reproductive tract and lead to inflammation [ 1 , 16 ]. Aside from bacterial contamination during calving, other pathogens can gain access to the reproductive organs of the cow during mating and artificial insemination via contaminated sources [ 17 – 20 ]. Transmission of the causative agents from close contact with the contaminated environmental source or infected hosts potentially leads to bovine reproductive diseases, such as leptospirosis, neosporosis, brucellosis and bovine viral diarrhoea [ 21 – 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

et al. 2021

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Undesirable microbial infiltration into the female bovine reproductive tracts, for example during calving or mating, is likely to disturb the commensal microflora. Persistent establishment and overgrowth of certain pathogens induce reproductive diseases, render the female bovine reproductive tract unfavourable for pregnancy or can result in transmission to the foetus, leading to death and abortion or birth abnormalities. This review of culture-independent metagenomics studies revealed that normal microflora in the female bovine reproductive tract is reasonably consistently dominated by bacteria from the phyla Bacteroidetes, Firmicutes, Proteobacteria, following by Actinobacteria, Fusobacteria and Tenericutes. Reproductive disease development in the female bovine reproductive tract was demonstrated across multiple studies to be associated with high relative abundances of bacteria from the phyla Bacteroidetes and Fusobacteria. Reduced bacterial diversity in the reproductive tract microbiome in some studies of cows diagnosed with reproductive diseases also indicated an association between dysbiosis and bovine reproductive health. Nonetheless, the bovine genital tract microbiome remains underexplored, and this is especially true for the male genital tract. Future research should focus on the functional aspects of the bovine reproductive tract microbiomes, for example their contributions to cattle fertility and susceptibility towards reproductive diseases.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

et al. 2021

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“…Campylobacteriosis is reported as a venereal disease with a worldwide distribution [ 47 ]. Reporting this pathogen in this metagenomic study of the samples collected from healthy bulls reanalyzes previous knowledge about the role of bulls in spreading venereal diseases while remaining asymptomatic [ 48 , 49 , 50 ]. The 16S rDNA metagenomic approach was applied in a case of human sperm microbiota where three main interaction bacterial modules were found, with Campylobacter being a part of one module together with other strictly anaerobic genera such as Prevotella, Finegoldia, Actinomyces, Fusobacterium, Dialister and Peptoniphilus [ 51 ], and it is reported to be a bacterial species associated with clinical criteria in a significant manner [ 52 ].…”

Section: Resultsmentioning

confidence: 88%

Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen

Medo

Žiarovská

Ďuračka

et al. 2021

Animals

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Bacterial contamination of semen is an important factor connected to the health status of bulls that may significantly affect semen quality for artificial insemination. Moreover, some important bovine diseases may be transmitted through semen. Up to now, only a very limited number of complex studies describing the semen microbiome of bulls have been published, as many bacteria are hard to cultivate using traditional techniques. The 16S rRNA high-throughput sequencing strategy allows for the reliable identification of bacterial profiles of bovine semen together with the detection of noncultivable bacterial species. Fresh samples from Holstein Friesian breeding bulls (n = 55) were examined for the natural variability in the present bacteria. Semen doses were selected randomly from Slovak Biological Services in Nitra, Slovak Republic. The most predominant phyla within the whole dataset were Firmicutes (31%), Proteobacteria (22%), Fusobacteria (18%), Actinobacteria (13%) and Bacteroidetes (12%). Samples of semen were divided into two separate clusters according to their microbiome compositions using a cording partition around a medoids analysis. Microbiomes of the first cluster (CL1) of samples (n = 20) were based on Actinobacteria (CL1 average = 25%; CL = 28%) and Firmicutes (CL1 = 38%; CL2 = 27%), while the second cluster (CL2; n = 35) contained samples characterized by a high prevalence of Fusobacteria (CL1 = 4%; CL2 = 26%). Some important indicator microbial groups were differentially distributed between the clusters.

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“…Bovine genital campylobacteriosis is transmitted primarily through natural mating in cattle breeding program; however, infection is also spread via infected bull semen used in artificial insemination (AI) and/contaminated equipment (Modolo et al., 2000 ). Transmitted between female cattle is less likely since the disease usually spreads through natural mating/coitus (Clark, 1971 ; Hoffer, 1981 ); however, transmission between bulls may occur during mounting when distance between breeding bulls is inadequate, and several bulls are kept in the same house (Taylor, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Campylobacter fetus in breeding bulls of private farms in Bangladesh

Hoque

Islam

Saddam

et al. 2022

Veterinary Medicine & Sci

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Background Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter fetus that has a negative impact on animal reproduction. The bull is considered to be a symptomless carrier that spreads the disease agent to breeding cows, causing infertility and sporadic abortion. Aim The study aims to estimate the prevalence, identify risk factors of Campylobacter fetus ( C. fetus ) infection and antimicrobial resistance pattern of the C. fetus isolates. Method A cross‐sectional survey was conducted in Mymensingh district of Bangladesh. Bull smegma samples (single sample from each bull) were collected from 300 bulls from four farms and tested via culture, biochemical identification and finally 16S rRNA and cdtA gene‐based molecular assays (PCR) for herd and animal‐level prevalence estimation. Herd‐ and animal‐level data on risk factors were collected from the farmers using a pretested questionnaire and analysed by univariable and multivariable logistic regression models with a p value of <0.05 was taken statistically significant for both analyses. Results Among the surveyed farms, 75% (95% CI: 19.4%–99.4%) were confirmed to have bulls infected with Campylobacter fetus at herd level. However, animal‐level occurrence of C. fetus was estimated to be 8.7% (26/300) (95% CI: 5.7%–12.4%). Natural service increases the odds of campylobacteriosis 38.18 times (95% CI: 13.89–104.94) in comparison to artificial insemination for C . fetus infection in bulls. Significantly, half of the isolates (50%, n = 13) were identified to be multidrug resistant (MDR) for three to five antimicrobial agents. Conclusion This study highlights the need to develop official guidelines for C. fetus control and prevention in Bangladesh including mandatory artificial insemination in reproductive cows and heifers, routine screening of breeding bulls for C. fetus free status.

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Occurrence of Campylobacter in the genitals of teaser bulls maintained at an embryo transfer center

Cited by 6 publications

References 1 publication

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen

Investigation of Campylobacter fetus in breeding bulls of private farms in Bangladesh

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