Aclimatização de Brassavola tuberculata com a utilização de ANA em diferentes substratos

Sousa, Gisele Garcia de; Rosa, Yara Brito Chaim Jardim; Macedo, Marichel Canazza de; Soares, Jackeline Schultz

doi:10.1590/s0102-053620150000200012

Cited by 13 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ouro Negro is also commonly used by commercial orchid growers in Tocantins. Sousa et al (2015) reported that the greatest survival percentage (74%) of Brassavola tuberculata plants during acclimatization was achieved when using sphagnum as a substrate when compared to coconut fiber and a mix of sphagnum and coconut fiber (1:1 v/v). They argued that due to its physical properties, sphagnum provided better water retention as well as adequate porosity.…”

Section: Acclimatizationmentioning

confidence: 99%

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

et al. 2018

View full text Add to dashboard Cite

Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival.

show abstract

Section: Acclimatizationmentioning

confidence: 99%

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

et al. 2018

View full text Add to dashboard Cite

show abstract

“…According to Miyata et al (2014), an efficient root growth process is related to high nitrogen, manganese and zinc concentrations in the composition of medium MS. The greater root formation presented by heterotrophic plants grown in vitro consisted in a desirable characteristic to improve acclimatization process efficacy, despite the low efficiency of the root system (Sousa et al, 2015).…”

Section: Values Depicted Inmentioning

confidence: 99%

Culture media for the multiplication of wild Manihot species

et al. 2018

View full text Add to dashboard Cite

The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 μmol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot.

show abstract

“…Significant differences were observed between acclimatization media, the SPAD values were higher using coir (24.81) compared with sphagnum (20.35) at 150 DAT, the LL was greater in sphagnum (6.63 cm) media comparing LL with coir (5.22 cm) at 30 DAT, and the EC was greater in coir (469.67 ms) compared with sphagnum (375.00 ms) at 150 DAT (Table 2). Sousa et al (2015) found no significant differences in the number of leaves and roots of Brassavola tuberculata for the substrates sphagnum and coconut fiber. The same was observed in this experiment, in which there was no significant difference between treatments for the number of leaves in T. undulatum.…”

Section: Resultsmentioning

confidence: 64%

Conservation Horticulture: In Vitro Micropropagation and Acclimatization of Selected Florida Native Orchids

Mullin

Costa

Downing

et al. 2022

horts

View full text Add to dashboard Cite

Florida is home to 106 native orchid species, of which 77 are listed as endangered or threatened by the State of Florida. The Institute for Regional Conservation (IRC) has classified 62 of these species as either critically imperiled, imperiled, or rare in South Florida. Because of lack of endosperm, orchid germination rates are very low in nature, as they depend on an obligate relationship with mycorrhizal fungi for nutrients. Most orchid seeds can be germinated in vitro without the need for specific mycorrhizal fungi. This study aims are to establish a fast and efficient protocol for in vitro seed germination using different nutrient media and plant growth regulator (PGR) combinations, and to optimize seedling acclimatization protocols using different greenhouse media. To determine germination preferences, three different in vitro seed germination media were tested supplemented with PGRs, including 1) Murashige & Skoog (MS) (control), 2) MS supplemented with 1.5 mg/L 6-benzylaminopurine (BAP), and 3) MS supplemented with 1 mg/L BAP and 0.5 mg/L 1-Naphthaleneacetic acid (NAA) on Cyrtopodium punctatum, a state-listed endangered, IRC critically imperiled epiphytic orchid. There was no significant difference amongst the treatments after 2 and 6 weeks of seed sowing culture. To understand post-culture survivorship, two epiphytic and one terrestrial native species (Trichocentrum undulatum, Encyclia tampensis, and Oncidium ensatum) were chosen for the acclimatization study with two commercially available potting substrates (coir, sphagnum). To measure seedling growth rates, phenotypic measurements [leaf number, leaf length, root length, plantlet height, light intensity, pH, and electrical conductivity (EC)] and Soil Plant Analysis Development (SPAD) and Normalized Difference Vegetation Index (NDVI) values were recorded monthly for five months. All media/PGR combinations resulted in an initial high percentage of stage I growth but inhibited Protocorm-like bodies (PLBs) development, suggesting more research is necessary to determine later improvements or detriments to MS basal media with BAP and NAA. Comparing acclimatization media with the three species of orchid chosen for this experiment, neither O. ensatum nor E. tampensis showed a marked preference for sphagnum moss or coir. However, T. undulatum did perform better with coir compared with sphagnum. This research will help botanical gardens and commercial plant tissue culture laboratories to have a better understanding on selection of PGR combinations for in vitro cell culture and acclimatization media on increasing the viability and plant health and decreasing the mortality of endangered plants.

show abstract

Aclimatização de Brassavola tuberculata com a utilização de ANA em diferentes substratos

Cited by 13 publications

References 10 publications

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

Culture media for the multiplication of wild Manihot species

Conservation Horticulture: In Vitro Micropropagation and Acclimatization of Selected Florida Native Orchids

Contact Info

Product

Resources

About