Mapping of serotonin-immunoreactive neurons of Anastrepha obliqua Macquart larvae

Boleli, Isabel Cristina; Paulino-Simoes, Zilil Luz

doi:10.1590/s0101-81751999000400019

Cited by 3 publications

(3 citation statements)

References 19 publications

(18 reference statements)

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“…, , , ). Three posterolateral neurons were detected in all thoracic ganglia of the flour beetle Tenebrio molitor (Breidbach, ) and exclusively in the prothoracic ganglion of numerous pterygote insects (Taghert and Goodman, ; Nässel and Cantera, ; Longley and Longley, ; Cantera and Nässel, ; Vallés and White, ; Radwan et al, ; Sivasubramanian, ; Boleli and Paulino‐Simões, ). More than two pairs of posterolateral neurons have never been described for Crustacea.…”

Section: Discussionmentioning

confidence: 99%

Serotonin‐containing neurons in basal insects: In search of ground patterns among tetraconata

Stemme¹,

Stern²,

Bicker³

2016

J of Comparative Neurology

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The ventral nerve cord of Tetraconata contains a comparably low number of serotonin-immunoreactive neurons, facilitating individual identification of cells and their characteristic neurite morphology. This offers the rather unique possibility of establishing homologies at the single cell level. Because phylogenetic relationships within Tetraconata are still discussed controversially, comparisons of individually identifiable neurons can help to unravel these issues. Serotonin immunoreactivity has been investigated in numerous tetraconate taxa, leading to reconstructions of hypothetical ground patterns for major lineages. However, detailed descriptions of basal insects are still missing, but are crucial for meaningful evolutionary considerations. We investigated the morphology of individually identifiable serotonin-immunoreactive neurons in the ventral nerve cord of Zygentoma (Thermobia domestica, Lepisma saccharina, Atelura formicaria) and Archaeognatha (Machilis germanica, Dilta hibernica). To improve immunocytochemical resolution, we also performed preincubation experiments with 5-hydroxy-L-tryptophan and serotonin. Additionally, we checked for immunolabeling of tryptophan hydroxylase, an enzyme associated with the synthesis of serotonin. Besides the generally identified groups of anterolateral, medial, and posterolateral neurons within each ganglion of the ventral nerve cord, we identified several other immunoreactive cells, which seem to have no correspondence in other tetraconates. Furthermore, we show that not all immunoreactive neurons produce serotonin, but have the capability for serotonin uptake. Comparisons with the patterns of serotonin-containing neurons in major tetraconate taxa suggest a close phylogenetic relationship of Remipedia, Cephalocarida, and Hexapoda, supporting the Miracrustacea hypothesis. J. Comp. Neurol., 2016. © 2016 Wiley Periodicals, Inc. J. Comp. Neurol. 525:79-115, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Serotonin‐containing neurons in basal insects: In search of ground patterns among tetraconata

Stemme¹,

Stern²,

Bicker³

2016

J of Comparative Neurology

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“…In insects, as in vertebrates and other invertebrates, serotonin is an important monoamine neurotransmitter that functions as a neural circuit modulator (Nässel, 1988; Qi et al, 2014; Vleugels et al, 2015). Antiserum against serotonin has been available for three decades, and the immunohistochemical experiments to investigate the distribution of serotonin in the nervous system have been performed in a large number of insect species, including locusts, mantes, cockroaches, bugs, beetles, ants, wasps, bees, flies, mosquitoes, and moths (Bishop and O’Shea, 1983; Tyrer et al, 1984; Lange et al, 1988; Nässel, 1988; Homberg and Hildebrand, 1989a, b; Breidbach, 1990; Boleli and Paulino-Simões, 1999; Leitinger et al, 1999; Settembrini and Villar, 2004; Dacks et al, 2006; Liu et al, 2011; Huser et al, 2012; van der Woude and Smid, 2017). Serotonin is distributed throughout the nervous system, including both peripheral and central regions.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Serotonin-Immunoreactive Neurons in the Brain and Gnathal Ganglion of Caterpillar Helicoverpa armigera

et al. 2019

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Serotonin (5-hydroxytryptamine, 5-HT) is an important biogenic amine that acts as a neural circuit modulator. It is widespread in the central nervous system of insects. However, little is known about the distribution of serotonin in the nervous system of the cotton bollworm Helicoverpa armigera . In the present study, we performed immunohistochemical experiments with anti-serotonin serum to examine the distribution of serotonin in the central nervous system of H. armigera larvae. We found about 40 serotonin-immunoreactive neurons in the brain and about 20 in the gnathal ganglion. Most of these neurons are wide-field neurons giving rise to processes throughout the neuropils of the brain and the gnathal ganglion. In the central brain, serotonin-immunoreactive processes are present bilaterally in the tritocerebrum, the deutocerebrum, and major regions of the protocerebrum, including the central body (CB), lateral accessory lobes (LALs), clamps, crepine, superior protocerebrum, and lateral protocerebrum. The CB, anterior ventrolateral protocerebrum (AVLP), and posterior optic tubercle (POTU) contain extensive serotonin-immunoreactive process terminals. However, the regions of mushroom bodies, the lateral horn, and protocerebral bridges (PBs) are devoid of serotonin-immunoreactivity. In the gnathal ganglion, the serotonin-immunoreactive processes are also widespread throughout the neuropil, and some process projections extend to the tritocerebrum. Our results provide the first comprehensive description of the serotonergic neuronal network in H. armigera larvae, and they reveal the neural architecture and the distribution of neural substances, allowing us to explore the neural mechanisms of behaviors by using electrophysiological and pharmacological approaches on the target regions.

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“…Serotonin [5-hydroxytryptamine (5-HT)] is a biogenic amine, acting as neurotransimitter, neuromodulator, or neurohormone in a wide range of organisms, including insects ( Nässel, 1988 ; Vleugels et al, 2015 ). The immunohistochemical experiments with serotonin antiserum showed that serotonergic neurons of insect species are limited in number, but the project processes were widely distributed in both the peripheral and the central nervous system ( Klemm et al, 1984 ; Lange et al, 1988 ; Nässel, 1988 ; Homberg and Hildebrand, 1989a , b ; Breidbach, 1990 ; Boleli and Paulino-Simões, 1999 ; Leitinger et al, 1999 ; Settembrini and Villar, 2004 ; Dacks et al, 2006 ; Liu et al, 2011 ; Huser et al, 2012 ; van der Woude and Smid, 2017 ; Tang et al, 2019 ; Tierney, 2020 ). The widespread serotonergic neurons are involved in multiple effects in a variety of behaviors and physiological activities, including vision, olfaction, audition, feeding, flight, aggregation, aggression, sleep, learning and memory, circadian rhythms, immunity, stress, metabolism, growth, and reproduction ( Kloppenburg and Mercer, 2008 ; Anstey et al, 2009 ; Qi et al, 2014 , 2016 ; Tang et al, 2019 ; Tierney, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Serotonergic Neurons in the Brain and Gnathal Ganglion of Larval Spodoptera frugiperda

et al. 2022

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The fall armyworm Spodoptera frugiperda (S. frugiperda) (Lepidoptera: Noctuidae) is a worldwide, disruptive, agricultural pest species. The larvae of S. frugiperda feed on seedling, leave, and kernel of crops with chewing mouthparts, resulting in reduced crop yields. Serotonin is an important biogenic amine acting as a neural circuit modulator known to mediate lots of behaviors including feeding in insects. In order to explore the serotonergic neural network in the nervous system of larval S. frugiperda, we performed immunohistochemical experiments to examine the neuropil structure of the brain and the gnathal ganglion with antisynapsin and to examine their serotonergic neurons with antiserotonin serum. Our data show that the brain of larval S. frugiperda contains three neuromeres: the tritocerebrum, the deutocerebrum, and the protocerebrum. The gnathal ganglion also contains three neuromeres: the mandibular neuromere, the maxillary neuromere, and the labial neuromere. There are about 40 serotonergic neurons in the brain and about 24 serotonergic neurons in the gnathal ganglion. Most of these neurons are wide-field neurons giving off processes in several neuropils of the brain and the gnathal ganglion. Serotonergic neuron processes are mainly present in the protocerebrum. A pair of serotonergic neurons associated with the deutocerebrum has arborizations in the contralateral antennal lobe and bilateral superior lateral protocerebra. In the gnathal ganglion, the serotonergic neuron processes are also widespread throughout the neuropil and some process projections extend to the tritocerebrum. These findings on the serotonergic neuron network in larval S. frugiperda allow us to explore the important roles of serotonin in feeding and find a potential approach to modulate the feeding behavior of the gluttonous pest and reduce its damage.

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Mapping of serotonin-immunoreactive neurons of Anastrepha obliqua Macquart larvae

Cited by 3 publications

References 19 publications

Serotonin‐containing neurons in basal insects: In search of ground patterns among tetraconata

Serotonin‐containing neurons in basal insects: In search of ground patterns among tetraconata

Distribution of Serotonin-Immunoreactive Neurons in the Brain and Gnathal Ganglion of Caterpillar Helicoverpa armigera

Serotonergic Neurons in the Brain and Gnathal Ganglion of Larval Spodoptera frugiperda

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