Rearing the larvae of the blowfly Chrysomya chloropyga (Wiedemann) (Diptera, Calliphoridae) on oligidic diets

Leal, Tania Teresinha de Souza; Prado, Angelo Pires do; Antunes, Aloisio Jose

doi:10.1590/s0101-81751982000100006

Cited by 49 publications

(39 citation statements)

References 4 publications

(2 reference statements)

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“…Eggs of the three species were collected from these cultures using ground raw bovine meat. Before hatching, the eggs were transferred from the meat to vials containing artificial culture medium (Leal et al 1982) growth. The vials were kept under the same conditions of the F1 cultures.…”

Section: Methodsmentioning

confidence: 99%

Distinction among the puparia of three blowfly species (Diptera: Calliphoridae) frequently found on unburied corpses

Amorim

Ribeiro

2001

Mem. Inst. Oswaldo Cruz

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Key words: Calliphoridae -carrion insects -decomposition -forensic entomology -pupariaThe family Calliphoridae consists of carrion flies which may also feed on living tissues. These species are potentially dangerous to man and other animals since the larvae may cause myiasis and adults may transmit pathogens. The diseases transmitted by these flies cause substantial losses to the cattle industry (Norris 1959, Zumpt 1965, Greenberg 1971, 1973, Richard & Gerrish 1983. The genus Chrysomya was introduced in Brazil from Africa (Zumpt 1965) with the first records being from the states of Paraná (Imbiriba et al. 1977) and São Paulo (Guimarães et al. 1978). Chrysomya species are currently found from the southern United States to southern Brazil (Jiron 1979, Gagné 1981, Baumgartner & Greenberg 1984.Insects are important in carcass decomposition, and calliphorids, which are among the most abundant and best studied carrion insects have been extensively used as indicators of the post mortem interval (death time) and of corpses translocation. These flies are therefore a valuable tool for forensic medicine (Megnin 1894, Smith 1986, Catts & Haskell 1990.In this study, we show that it is possible to distinguish among three species (C. megacephala, C. putoria and Cochliomyia macellaria) based on characteristics of their pupae and puparia. MATERIALS AND METHODSCultures (F1) of each species were maintained in cages (25 cmx25 cmx30 cm) covered with mosquito nets, at 25 ± 1 o C, 60% relative humidity and a 12 h photoperiod. The flies were fed raw bovine liver for four days immediately after emergence and with water and sugar ad libitum thereafter. Eggs of the three species were collected from these cultures using ground raw bovine meat. Before hatching, the eggs were transferred from the meat to vials containing artificial culture medium (Leal et al. 1982) growth. The vials were kept under the same conditions of the F1 cultures. When the larvae reached the third instar, they were transferred to vials filled with sawdust for pupating.After the F2 adults of each species had emerged, specimens of their puparia (without any cleaning) were examined with a binocular microscope to assess interspecific differences in the posterior spiracle region. In 30 puparia from each species, the distance between the two peritrema was measured. These distances were compared by analysis of variance followed by the Student-Newman-Keuls test (Zar 1996), with the critical value set at p = 0.05. RESULTSC. putoria puparia had six very conspicuous tubercles in the perispiracular region whereas in

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Section: Methodsmentioning

confidence: 99%

Distinction among the puparia of three blowfly species (Diptera: Calliphoridae) frequently found on unburied corpses

Amorim

Ribeiro

2001

Mem. Inst. Oswaldo Cruz

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“…Twenty different larval densities ranging from 100 to 2,000 larvae with intervals of 100 were established using 50,000 mg of rearing medium per vial (7.2 cm diameter x 13.8 cm height), prepared according to Leal et al (1982). These larval densities are equivalent from 2 to 40 individuals per g. The rearing medium was chosen in order to control the amount of protein content and to avoid extraneous variation that might be introduced with other media such as liver or carcass.…”

Section: Methodsmentioning

confidence: 99%

Theoretical estimates of consumable food and probability of acquiring food in larvae of Chrysomya putoria (Diptera: Calliphoridae)

Godoy

Zuben

Reis

et al. 1996

Mem. Inst. Oswaldo Cruz

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“…In the simulations we used the values obtained from Table 2, which were statistically estimated from data acquired in laboratory experiments (Table 1). , (Table 1) were established using newly hatched larvae in 50 g of rearing medium prepared according to Leal et al (1982), with two replicates. Fecundity was estimated by randomly removing 30 females per replicate and counting the number of eggs per female.…”

Section: Mathematical Modelsmentioning

confidence: 99%

Allee effect in exotic and introduced blowflies

Serra¹,

Costa²,

Godoy³

2011

Neotrop. entomol.

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We combined two models to investigate the theoretical dynamics of five exotic and native blowfly species in response to the Allee effect by using demographic parameters estimated from experimental populations. Most of the results suggest stabilization of dynamic behavior in response to the Allee effect. However, the results depended on the magnitude of the demographic parameters of each species, and also indicated chaotic fluctuations and limit cycles. The results are discussed in the context of larval aggregation, an important biological process for blowflies, which naturally incorporates the Allee effect.

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Rearing the larvae of the blowfly Chrysomya chloropyga (Wiedemann) (Diptera, Calliphoridae) on oligidic diets

Cited by 49 publications

References 4 publications

Distinction among the puparia of three blowfly species (Diptera: Calliphoridae) frequently found on unburied corpses

Distinction among the puparia of three blowfly species (Diptera: Calliphoridae) frequently found on unburied corpses

Theoretical estimates of consumable food and probability of acquiring food in larvae of Chrysomya putoria (Diptera: Calliphoridae)

Allee effect in exotic and introduced blowflies

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