Imagens digitalizadas na interpretação do teste de tetrazólio em sementes de Brachiaria brizantha

Custódio, Ceci Castilho; Damasceno, Rogério Lopes; Neto, Nelson Barbosa Machado

doi:10.1590/s0101-31222012000200020

Cited by 12 publications

(7 citation statements)

References 19 publications

(5 reference statements)

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“…The percentages of viable seeds detected in the 15 lots of U. decumbens seeds were similar to those found in other seedlings of this genus (CUSTÓDIO et al, 2012;QUADROS et al 2012;CARDOSO et al, 2014;PEREIRA et al, 2014). Quadros et al (2012) considered that the average of 68.4% of seed viability was satisfactory for U. brizantha seeds of Marandú and Xaráes cultivars; thus, verifying the high viability of evaluated lots.…”

Section: Resultssupporting

confidence: 75%

Production regions and physical quality of Urochloa decumbens CV. Basilisk seeds

Silva¹,

Martins²,

Cruz³

et al. 2019

Biosci. J.

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Brazil is the largest world producer, consumer and exporter of forage grass seeds. Urochloa decumbens Stapf. species is the second in importance in the Brazilian market of seeds of tropical forage grasses. Seeds are harvested by the soil sweeping method and, in addition to other impurities, harvested lots can present weed seeds, which makes seeds unfeasible for commercialization. The aim of the present study was to evaluate differences in the physical quality and viability of Urochloa decumbens cv. Basilisk seeds produced in different production fields. Fifteen U. decumbens seed lots from the State of São Paulo (1 lot in the municipality of Cássia dos Coqueiros and 3 lots in the municipality of Santo Antônio da Alegria), Minas Gerais (2 lots in the municipality of Tupaciguara, 2 lots in the municipality of Unaí, 2 lots in the municipality of Chapada Gaúcha and 3 lots in the municipality of Monte Santo de Minas), Goiás (1 lot in the municipality of Jataí) and Mato Grosso (1 lot in the municipality of Primavera do Leste) were evaluated by means of the following parameters: water content, viability by tetrazolium test, purity, determination of other seeds by number and weight of one thousand seeds. A completely randomized design was used, and when significant, the means of treatments were compared by the Scott Knott test at 5% probability. The fields of the different regions produce U. decumbens seeds with high variability in physical quality. U. decumbens seeds with the highest physical quality and viability were produced in Cassia dos Coqueiros-SP.

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Section: Resultssupporting

confidence: 75%

Production regions and physical quality of Urochloa decumbens CV. Basilisk seeds

Silva¹,

Martins²,

Cruz³

et al. 2019

Biosci. J.

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“…As imagens escaneadas permitiram identificação de lotes com sementes menos vigorosas, não interferindo na caracterização de coloração cinza a branco leitoso, própria de tecidos não vivos (Figura 3). O que corrobora com os resultados obtidos por Custódio et al (2012) com interpretação de tetrazólio em sementes de Brachiaria brizantha por meio de análise de imagens digitalizadas, evidenciando a eficiência da interpretação equivalente ao emprego de estéreo microscópio.…”

Section: Lotesunclassified

“…Em sementes de Brachiaria brizantha, Custódio et al (2012), observaram que análise de imagens digitalizada para interpretação do teste de tetrazólio, por meio de scanner, equivale à análise por observação em estéreo microscópio. A técnica mostrou-se eficiente na avaliação, além de garantir maior rapidez, facilidade, possibilidade de ampliação e armazenamento de imagens.…”

Section: Introductionunclassified

Imagens digitalizadas na avaliação do teste de tetrazólio em sementes de trigo

et al. 2020

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The tetrazolium test can be an alternative to obtain fast results of the physiological potential of wheat seeds. In this context, the objective of the present study was to evaluate the efficiency of the tetrazolium test through the evaluation by analysis of digitized images in the determination of the physiological quality of wheat seeds.The experiment was carried out with 22 lots of wheat seeds, submitted to the determination of the physiological potential, including the tetrazolium test evaluated by means of digitized image analysis.For digitization, after longitudinal bisection, and staining in 0.075% tetrazolium solution, the seeds were grouped on tabletop scanner glass, stored and classified into four vigor classes. Data were submitted to analysis of variance, with means grouped by the Scott-Knott test, at 5% of significance; we also performed an analysis of the simple correlation coefficients between tetrazolium test results and other tests.The evaluation of the tetrazolium test through the analysis of digitized images grouped the lots in three levels of vigor.The method is effective in determining the vigor and viability of wheat seeds, because the possibility of expanding the images that allows precise analysis of the embryonic axis structures.

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“…A viabilidade das sementes foi avaliada através do teste de tetrazólio utilizando 4 repetições de 25 sementes seguindo a metodologia proposta por (CUSTÓDIO et al, 2012). Após a coloração dos embriões foi realizada a avaliação para a identificação e contagem das sementes com as partes vitais do embrião coloridas (viáveis) (BRASIL, 2009), e os resultados expressos em porcentagem de sementes viáveis.…”

Section: Metodologiaunclassified

AVALIAÇÃO DA DORMÊNCIA DE Urochloa humidicola CV. LLANERO SOB DIFERENTES DOSES DE ÁCIDO GIBERÉLICO E PACLOBUTRAZOL

Sorigotti¹,

Garcia²,

Custódio³

et al. 2016

COLLOQ AGRARIAE

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RESUMOO objetivo foi avaliar a germinação e dormência das sementes de U. humidicola cv. Llanero em diferentes concentrações de ácido giberélico e paclobutrazol, utilizando sementes intactas e sementes nuas (cariopses). Utilizou-se diferentes soluções para embebição do substrato. As soluções de ácido giberélico (GA 3 ) foram: 0 (água pura); álcool 5%; 0,50 mM L -1 ; 1,0 mM L -1 ; 2,0 mM L -1 e 4,0 mM L -1 e de paclobutrazol (PBZ): 0; 0,05 g L -1 ; 0,100 g L -1 e 0,200 g L -1 . Avaliou-se a germinação por protrusão de raiz primária, índice de velocidade de germinação e a porcentagem de dormência das sementes. Os dados foram analisados pelo teste F e o teste de Tukey para comparação de médias. O GA 3 favorece a germinação das sementes intactas, porém para a germinação do cariopse não há influência do ácido giberélico. O PBZ não inibe a germinação das sementes nuas. A germinação esta relacionada à outros fatores, além do balanço de giberelinas nas sementes. Palavras-chave: Germinação; Giberelina; Inibidor da giberelina; Triazol; Urochloa. EVALUATION DORMANCY Urochloa humidicola CV. LLANERO UNDER DIFFERENT ACID DOSES GIBBERELLIC AND PACLOBUTRAZOLThe objective was to evaluate the germination and dormancy of U. humidicola seeds cv. Llanero in different concentrations of gibberellic acid and paclobutrazol using intact seeds and naked seeds (caryopses). Different solutions are used for soaking the substrate. The solution of gibberellic acid (GA3) were: 0 (pure water) ; 5% alcohol; 0.50 mM L-1; 1.0 mM L-1; 2.0 mM L-1 and 4.0 mM L-1 and paclobutrazol (PBZ): 0; 0.05 g L-1 ; 0.100 g L-1 and 0.200 g L-1 . We evaluated the germination protrusion of primary root, germination speed index and the percentage of seed dormancy. Data were analyzed by F test and Tukey test to compare means. The GA3 favors the germination of intact seeds, but for the germination of caryopses there is no influence of gibberellic acid. The PBZ does not inhibit the germination of naked seeds. Germination is related to other factors and the balance of gibberellins in the seeds.

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Imagens digitalizadas na interpretação do teste de tetrazólio em sementes de Brachiaria brizantha

Cited by 12 publications

References 19 publications

Production regions and physical quality of Urochloa decumbens CV. Basilisk seeds

Production regions and physical quality of Urochloa decumbens CV. Basilisk seeds

Imagens digitalizadas na avaliação do teste de tetrazólio em sementes de trigo

AVALIAÇÃO DA DORMÊNCIA DE Urochloa humidicola CV. LLANERO SOB DIFERENTES DOSES DE ÁCIDO GIBERÉLICO E PACLOBUTRAZOL

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