“…The experiment was carried out in two phases: depletion, to induce anemia, and hemoglobin repletion, to recover it. In the depletion phase (Association of Official Analytical Chemists, 1997; Enes et al, 2014), the animals fed a diet with free-iron mineral mix to induce irondeficiency anemia, based on the AIN-93G diet (Reeves et al, 1993). Deionized water was offered ad libitum.…”