Evaluation of the PetrifilmTM and TEMPO® systems and the conventional method for counting microorganisms in pasteurized milk

Cirolini, Andréia; Baseggio, Andressa Mara; Miotto, Marília; Ramos, Roberta Juliano; Cattani, Cristhiane Stecanella de Oliveira; Vieira, Cleide Rosana Werneck

doi:10.1590/s0101-20612013000400026

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Despite delivering high-throughput analysis (100 enumerations/hour), sensitivity may not be suitable for all kinds of microbiological analysis (detection range: 10 2 -10 8 CFU/g) (BioMérieux 2018). Such an instrument ensures considerable time and labor saving, performing well in food analysis in comparison with standard methods (Owen, Willis, and Lamph 2010;Paulsen et al 2008), but poor accuracy compared to plate count reference method was reported for some analyses in certain food matrices (Cirolini et al 2013).…”

Section: Conventional Methods For the Assessment Of Microbiological F...mentioning

confidence: 99%

How to Warrant Microbiological Food Safety in Food Companies: Problems and a Possible Solution

Antonini¹,

Stalio²,

Arienzo³

et al. 2018

Food Studies: An Interdisciplinary Journal

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The Food Studies Research Network recognizes the contribution of many in the evolution of the Research Network. The principal role of the Advisory Board has been, and is, to drive the overall intellectual direction of the Research Network. A full list of members can be found at http://food-studies.com/about/advisory-board. PEER REVIEWArticles published in Food Studies: An Interdisciplinary Journal are peer reviewed using a two-way anonymous peer review model.

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Section: Conventional Methods For the Assessment Of Microbiological F...mentioning

confidence: 99%

How to Warrant Microbiological Food Safety in Food Companies: Problems and a Possible Solution

Antonini¹,

Stalio²,

Arienzo³

et al. 2018

Food Studies: An Interdisciplinary Journal

View full text Add to dashboard Cite

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Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices

Papkovsky

Kerry

2023

Sensors

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The current status of microbiological testing methods for the determination of viable bacteria in complex sample matrices, such as food samples, is the focus of this review. Established methods for the enumeration of microorganisms, particularly, the ‘gold standard’ agar plating method for the determination of total aerobic viable counts (TVC), bioluminescent detection of total ATP, selective molecular methods (immunoassays, DNA/RNA amplification, sequencing) and instrumental methods (flow cytometry, Raman spectroscopy, mass spectrometry, calorimetry), are analyzed and compared with emerging oxygen sensor-based respirometry techniques. The basic principles of optical O2 sensing and respirometry and the primary materials, detection modes and assay formats employed are described. The existing platforms for bacterial cell respirometry are then described, and examples of particular assays are provided, including the use of rapid TVC tests of food samples and swabs, the toxicological screening and profiling of cells and antimicrobial sterility testing. Overall, O2 sensor-based respirometry and TVC assays have high application potential in the food industry and related areas. They detect viable bacteria via their growth and respiration; the assay is fast (time to result is 2–8 h and dependent on TVC load), operates with complex samples (crude homogenates of food samples) in a simple mix-and-measure format, has low set-up and instrumentation costs and is inexpensive and portable.

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Matrix-Specific Method Validation of an Automated Most-Probable-Number System for Use in Measuring Bacteriological Quality of Grade “A” Milk Products

Lindemann

Kmet

Reddy

et al. 2016

Journal of Food Protection

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The U.S. Food and Drug Administration (FDA) oversees a long-standing cooperative federal and state milk sanitation program that uses the grade "A" Pasteurized Milk Ordinance standards to maintain the safety of grade "A" milk sold in the United States. The Pasteurized Milk Ordinance requires that grade "A" milk samples be tested using validated total aerobic bacterial and coliform count methods. The objective of this project was to conduct an interlaboratory method validation study to compare performance of a film plate method with an automated most-probable-number method for total aerobic bacterial and coliform counts, using statistical approaches from international data standards. The matrix-specific validation study was administered concurrently with the FDA's annual milk proficiency test to compare method performance in five milk types. Eighteen analysts from nine laboratories analyzed test portions from 12 samples in triplicate. Statistics, including mean bias and matrix standard deviation, were calculated. Sample-specific bias of the alternative method for total aerobic count suggests that there are no large deviations within the population of samples considered. Based on analysis of 648 data points, mean bias of the alternative method across milk samples for total aerobic count was 0.013 log CFU/ml and the confidence interval for mean deviation was -0.066 to 0.009 log CFU/ml. These results indicate that the mean difference between the selected methods is small and not statistically significant. Matrix standard deviation was 0.077 log CFU/ml, showing that there is a low risk for large sample-specific bias based on milk matrix. Mean bias of the alternative method was -0.160 log CFU/ml for coliform count data. The 95% confidence interval was -0.210 to -0.100 log CFU/ml, indicating that mean deviation is significantly different from zero. The standard deviation of the sample-specific bias for coliform data was 0.033 log CFU/ml, indicating no significant effect of milk type.

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Evaluation of the PetrifilmTM and TEMPO® systems and the conventional method for counting microorganisms in pasteurized milk

Cited by 3 publications

References 24 publications

How to Warrant Microbiological Food Safety in Food Companies: Problems and a Possible Solution

How to Warrant Microbiological Food Safety in Food Companies: Problems and a Possible Solution

Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices

Matrix-Specific Method Validation of an Automated Most-Probable-Number System for Use in Measuring Bacteriological Quality of Grade “A” Milk Products

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