Elongated cells of Listeria monocytogenes in biofilms in the presence of sucrose and bacteriocin-producing Leuconostoc mesenteroides A11

Ratti, Regiane Priscila; Gomes, Bruna Carrer; Martinez, Raquel Chacon Ruiz; Souza, Vanessa Maciel de; Martinis, Elaine Cristina Pereira De

doi:10.1590/s0101-20612010000400027

Cited by 17 publications

(9 citation statements)

References 11 publications

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“…Such an altered morphology of L . monocytogenes has previously been reported because of the suboptimum conditions of the environment or different stresses [ 47 , 48 ]. In contrast, Wen et al [ 49 ] reported that such a change could be because of the transition from the log to the long-term survival phase.…”

Section: Discussionmentioning

confidence: 99%

Biofilm-Forming Abilities of Listeria monocytogenes Serotypes Isolated from Different Sources

et al. 2015

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A total of 98 previously characterized and serotyped L. monocytogenes strains, comprising 32 of 1/2a; 20 of 1/2b and 46 of 4b serotype, from clinical and food sources were studied for their capability to form a biofilm. The microtiter plate assay revealed 62 (63.26%) strains as weak, 27 (27.55%) strains as moderate, and 9 (9.18%) strains as strong biofilm formers. Among the strong biofilm formers, 6 strains were of serotype 1/2a and 3 strains were of serotype 1/2b. None of the strain from 4b serotype exhibited strong biofilm formation. No firm correlation (p = 0.015) was noticed between any serotype and respective biofilm formation ability. Electron microscopic studies showed that strong biofilm forming isolates could synthesize a biofilm within 24 h on surfaces important in food industries such as stainless steel, ceramic tiles, high-density polyethylene plastics, polyvinyl chloride pipes, and glass. Cell enumeration of strong, moderate, and weak biofilm was performed to determine if the number of cells correlated with the biofilm-forming capabilities of the isolates. Strong, moderate, and weak biofilm showed 570±127× 103 cells/cm2, 33±26× 103 cells/cm2, 5±3× 103 cells/cm2, respectively, indicating that the number of cells was directly proportional to the strength of the biofilm. The hydrophobicity index (HI) analysis revealed higher hydrophobicity with an increased biofilm formation. Fatty acid methyl esterase analysis revealed the amount of certain fatty acids such as iso-C15:0, anteiso-C15:0, and anteiso-C17:0 fatty acids correlated with the biofilm-forming capability of L. monocytogenes. This study showed that different strains of L. monocytogenes form biofilm of different intensities which did not completely correlate with their serotype; however, it correlated with the number of cells, hydrophobicity, and amount of certain fatty acids.

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Section: Discussionmentioning

confidence: 99%

Biofilm-Forming Abilities of Listeria monocytogenes Serotypes Isolated from Different Sources

et al. 2015

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“…Importantly, the strain tested in the present study is a strong biofilm-producer [13], and had intermediate susceptibility to PAA treatment (IZ = 8.7 ± 1.2), which is a chemical sanitizer commonly used worldwide. This fact may contribute for the ability of L. monocytogenes to survive in biofilms formed on equipments and instruments in food industries [20]. Thus the entrance of this pathogen in food processing areas does not only involve an immediate risk to food safety, but also the risk of a long-term persistence unless appropriate measurements are applied to eliminate the risk [21].…”

Section: Methodsmentioning

confidence: 99%

Effects of Oleuropein and Peracetic Acid as Sanitizing Agents for Inactivation of Biofilms

Dominciano¹,

Lee²,

Corassin³

et al. 2016

Open Conf. Proceed. J.

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The development of more efficient sanitizers for reducing the biofilm produced by pathogenic bacteria is of great importance, especially in the food industry. The aim of this study was to conduct a preliminary evaluation of the efficiency of oleuropein (OLE), extracted from olive leaves, and peracetic acid (PAA), alone or in combination, to inactivate biofilms formed by a strong biofilm-producer strain of Listeria monocytogenes (ATCC 7644). A disk diffusion method was also used to assess the susceptibility of the ATCC 7644 strain cells to OLE and/or PAA. Triplicate biofilm assays were prepared on stainless steel coupons (1x1 cm 2) during 48 h without stirring. After incubation, the stainless steel was washed (NaCl 0.85%) and immersed in solutions of OLE (5.0 mg/mL) and/or PAA (2.0%) for 1 min. After 1-min treatment, the disinfectant solutions were removed from the tube. Two mL of sodium thiosulfate 0.1 M was placed in the tube to stop reaction. The inactivation of biofilms was assessed by confocal laser scanning microscopy. L. monocytogenes cells tested in the disk diffusion method was not susceptible to OLE (inhibition zone (IZ) = 6 mm), although its susceptibility was intermediate for PAA (IZ = 8.67 mm) and higher for PAA+OLE (IZ = 14 mm). Compared with PAA, OLE alone in contact with biofilms had also lower bactericidal activity on the biofilms. However, the treatment of OLE in combination with PAA resulted in greater inactivation of L. monocytogenes cells in biofilms. Results indicate a potential application of OLE for enhancing the bactericidal effect of PAA against L. monocytogenes biofilms, although further studies are necessary to understand the mechanisms of action of OLE in combination with commercial chemical sanitizers.

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“…Studies showed that bacteriocin produced by Lactobacillus plantarum 35d, Enterococcus casseliflavus IM 416K1, Lactococcus lactis UQ2 and Lactococcus lactis possess the ability to control biofilm formed by L. monocytogenes . Similar study shows that the effectiveness of bacteriocin producing Leuconostoc mesenteriodes to delay the biofilm formation in L. monocytogenes on stainless steel surface .…”

Section: Control Of L Monocytogenes Biofilm Formationmentioning

confidence: 65%

Inhibition strategies of Listeria monocytogenes biofilms—current knowledge and future outlooks

Oloketuyi

2017

J Basic Microbiol

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There is an increasing trend in the food industry on the Listeria monocytogenes biofilm formation and inhibition. This is attributed to its easy survival on contact surfaces, resistance to disinfectants or antibiotics and growth under the stringent condition used for food processing and preservation thereby leading to food contamination products by direct or indirect exposure. Though, there is a lack of conclusive evidences about the mechanism of biofilm formation, in this review, the concept of biofilm formation and various chemical, physical, and green technology approaches to prevent or control the biofilm formed is discussed. State-of-the-art approaches ranging from the application of natural to synthetic molecules with high effectiveness and non-toxicity targeted at the different steps of biofilm formation could positively influence the biofilm inhibition in the future.

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Elongated cells of Listeria monocytogenes in biofilms in the presence of sucrose and bacteriocin-producing Leuconostoc mesenteroides A11

Cited by 17 publications

References 11 publications

Biofilm-Forming Abilities of Listeria monocytogenes Serotypes Isolated from Different Sources

Biofilm-Forming Abilities of Listeria monocytogenes Serotypes Isolated from Different Sources

Effects of Oleuropein and Peracetic Acid as Sanitizing Agents for Inactivation of Biofilms

Inhibition strategies of Listeria monocytogenes biofilms—current knowledge and future outlooks

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