Resistência antimicrobiana de Pseudomonas aeruginosa isolados de pescado e de cortes e de miúdos de frango

Abstract

Presente no meio ambiente, Pseudomonas aeruginosa é ubiquitário da água e solo, podendo formar biofilmes em algumas superfícies ou substratos. É reconhecido como pertencente à microbiota normal da superfície de plantas, pele do homem e animais, porém sua relevância está em seu papel como patógeno oportunista, ocasionando infecções quando da redução dos mecanismos de defesa do hospedeiro.Isto é exacerbado quando estes microrganismos têm acesso a sítios corporais normalmente estéreis, nos quais P. aeruginosa pode determinar infecções, como no trato urinário, sistema respiratório, sítios cirúrgicos, queimaduras graves, dermatites, estando algumas vezes associado com casos de meningite, endocardite, episódios de diarréia e septicemia; quadros clínicos também observados em diferentes espécies animais, especial- AbstractPseudomonas aeruginosa strains isolated from fish and chicken products were analyzed using the Antimicrobial Susceptibility Test with 14 drugs to evaluate the susceptibility patterns of this microorganism. Strains isolated from two different sources were evaluated. The fish strains belonged to the collection of Bacterioses Laboratory/IV/UFRRJ, and those from chicken specimens were isolated from the liver and chicken carcasses traded in commercial establishments in the city of Rio de Janeiro. The isolation methodology included enrichment in Peptone Water and then spread on EMB Agar and GSP Agar. The fish strains were inoculated into Peptone Water and then spread on EMB Agar. The suggestive colonies were streaked onto TSI and LIA Agar for metabolic evaluation. The activity of the green pigments was evaluated by Muller-Hinton Agar and the production of oxydase enzyme by Nutrient Agar. The antimicrobial susceptibility test performed for the 63 strains revealed the resistance to NAL and NIT (96.8%), TCY (93.6%), AMC (92.1%), CHL (90.5%), and SXT (85.7%) highlighting multi-resistance of the isolate. All strains isolated from chicken were susceptible to ATM and CAZ, and the strains isolated from fish were susceptible to ATM, CAZ, IPM, and AMK. Keywords: food; animal source; antimicrobial resistance. ResumoPseudomonas aeruginosa isolados de peixes de água doce e de frangos foram submetidos ao teste de suscetibilidade aos antimicrobianos utilizando quatorze drogas, com o objetivo de determinar e confrontar os padrões de suscetibilidade deste microrganismo. As cepas oriundas de peixes pertenciam à coleção do Laboratório de Bacterioses/IV/UFRRJ. Para o isolamento das cepas, foram selecionados miúdos (fígado) e cortes (coxa e sobrecoxa) de frangos adquiridos em estabelecimentos comerciais no município do Rio de Janeiro. A metodologia de isolamento incluiu o enriquecimento em água peptonada, seguido de semeadura em Agar EMB e Agar GSP. Para as cepas oriundas de peixes, procedeu-se à reativação em água peptonada, seguida de reisolamento em Agar EMB. Colônias sugestivas foram transferidas para Agar TSI e LIA para avaliação das características metabólicas. A capacidade de produção de pigmento verde-azulado foi avaliad...

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