Composition and physicochemical properties of two protein fractions of bovine blood serum

Prata, Ana Silvia; Sgarbieri, Valdemiro Carlos

doi:10.1590/s0101-20612008000400032

Cited by 13 publications

(7 citation statements)

References 29 publications

(22 reference statements)

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“…Other studies evaluated the amino acids profile of RBPC showing some variations, probably, due to the protein extraction procedure employed (TANG et al, 2003;HAN et al, 2015). Comparing the RBPC amino acids profile with BSA, lower amino acids content was observed for RBPC, an expected condition, since the BSA is a protein of high biological value (PRATA & SGARBIERI, 2008).…”

Section: Rbpc Composition and Colormentioning

confidence: 99%

Feasibility of ultrasound-assisted optimized process of high purity rice bran protein extraction

et al. 2020

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Rice bran is a by-product of the rice milling process, found worldwide in abundance and highlighted due its protein content. This study optimized the conditions for ultrasonic-assisted extraction of protein from defatted rice bran (DRB) and characterized the rice bran protein concentrate (RBPC). A sequential strategy of experimental design was employed; the effect of pH, temperature, ultrasound exposure time, and amplitude were evaluated regarding the percentage of protein extraction by a Full factorial design (FFD) with a fixed frequencies (FFD-A: 37 kHz; FFD-B: 80 kHz). Subsequently, the percentage of protein extracted was optimized employing a Central composite rotatable design (variables: pH and ultrasound exposure time) and RBPC obtained was characterized regarding chemical and functional properties. The pH and ultrasound exposure time had positive effect (P ≤ 0.05) on percentage of protein extraction; moreover, 37 kHz frequency was more effective in protein extraction. The optimized condition (frequency: 37 kHz; temperature: 30 °C; pH: 10; ultrasound exposure time: 30 min; and amplitude: 100%) allowed 15.07% of protein recovery and the RBPC presented 84.76 g 100 g-1 of protein. Magnesium and copper were the main mineral in RBPC (34.4 and 25.5 µg g-1, respectively), while leucine was the limiting amino acid (0.42) and threonine presented the highest chemical score (1.0). The RBPC solubility was minimal at pH 4 and higher at pHs 6-10; the water and oil absorption capacity were higher than bovine serum albumin (BSA) and the emulsifying capacity was comparable to BSA, with a suitable stability. It was possible to obtain a higher purity RBPC than described in the literature, due to the optimization in the extraction process steps, with functional properties suitable for application in food products, especially emulsified ones.

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Section: Rbpc Composition and Colormentioning

confidence: 99%

Feasibility of ultrasound-assisted optimized process of high purity rice bran protein extraction

et al. 2020

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“…As a result, one could speculate that charge transfer from the ligand (BSA) to the metal (Au) core [ligand to metal charge transfer (LMCT)] through the Au−S bonds and direct donation of delocalized electrons of electron rich atoms or groups of the ligand to the metal core could be a plausible reason for the luminescent properties of the GNC. As cysteine is one of the amino acids present in BSA 36 that contains a SH group, upon synthesis of the GNC this SH group forms the well-known gold−sulfur interface and LMCT takes place through the Au−S bond. The quantum yield (QY) of 6%, the fluorescence lifetime of 5.4 ns (Figure 2d), and the potential low toxicity 30 of the GNC allow for its use as a fluorescent probe for SRM imaging of lysosomes in HeLa cells.…”

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confidence: 99%

Bovine Serum Albumin-Conjugated Red Emissive Gold Nanocluster as a Fluorescent Nanoprobe for Super-resolution Microscopy

Yadav

Verma

Rao

et al. 2020

J. Phys. Chem. Lett.

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The gold nanocluster (GNC), because of its interesting photoluminescence properties and easy renal clearance from the body, has tremendous biomedical applications. Unfortunately, it has never been explored for super-resolution microscopy (SRM). Here, we present a protein-conjugated red emissive GNC for super-resolution radial fluctuation (SRRF) of the lysosome in HeLa cells. The diameter of the lysosome obtained in SRRF is ∼59 nm, which is very close to the original diameter of the smallest lysosome in HeLa cells. Conjugation of protein to GNC aided in the specific labeling of the lysosome. We hope that GNC not only will replace some of the common dyes used in SRM but due to its electron beam contrast could also be used as a multimodal probe for several other correlative bioimaging techniques.

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“…When pH increases from 9 to 11, the −NH þ 3 on the side chains of lysine convert to −NH 2 , further increasing the labeling efficiency. According to Table 1, at pH 11, the increase in labeling efficiency compared with pH 9 is greater than the content of lysine in BSA, which is about 12% as reported [42]. This excessive increment may be attributed to two possibilities.…”

Section: Verification Of Improved Labeling Efficiency By Bsamentioning

confidence: 75%

Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

et al. 2021

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Abstract1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is widely used as a crosslinker for fluorescence labeling of protein in the fields of biochemistry and food analysis. Many natural polysaccharides often contain some proteins or peptides that are very low in content but play a vital role in their biological function as well as technical applications. Determination of these low-content proteinaceous matters requires a highly sensitive and selective method. In this study, a methodological approach for investigations of the presence of proteinaceous material over the molar mass distribution (MD) of polysaccharides was developed using gum acacia (GA) as a model polysaccharide. EDC fluorescence-labeling method was modified by changing the pH (7, 9, and 11) of the solution for the analysis of low-content protein in food materials. Fluorescence spectroscopy and asymmetrical flow field-flow fractionation (AF4) were employed for characterizing the labeling efficiency and physiochemical properties of unlabeled and fluorescence-labeled GA. AF4 provided molar mass (M) and the radius of gyration (rG) of arabinogalactan (AG) and arabinogalactan protein complex (AGP) and determined the presence of proteinaceous matter over the MD. The labeling efficiencies of GA at pH 7, 9, and 11 determined by fluorescence spectroscopy were 56.5, 68.4, and 72.0%, respectively, with an increment of 15.5% when pH was increased from 7 to 11. The modified EDC fluorescence-labeling method allows highly sensitive and selective analysis of low-content proteinaceous matters and their distribution in natural polysaccharides. Graphical abstract

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Composition and physicochemical properties of two protein fractions of bovine blood serum

Cited by 13 publications

References 29 publications

Feasibility of ultrasound-assisted optimized process of high purity rice bran protein extraction

Feasibility of ultrasound-assisted optimized process of high purity rice bran protein extraction

Bovine Serum Albumin-Conjugated Red Emissive Gold Nanocluster as a Fluorescent Nanoprobe for Super-resolution Microscopy

Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

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