Vibrio parahaemolyticus produtores de urease isolados a partir de ostras (Crassostrea rizophorae) coletadas in natura em restaurantes e mexilhões (Perna perna) de banco natural

Pereira, Chistiane Soares; Viana, Célio Mauro; Rodrigues, Dália dos Prazeres

doi:10.1590/s0101-20612004000400019

Cited by 15 publications

(15 citation statements)

References 10 publications

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“…Os sorotipos mais comumente identificados no Brasil nas duas últimas décadas foram O2:K28, O3:K5, O10:K52, O5:K17 e O4:K12 10 . O primeiro relato de identificação do sorotipo O3:K6 em nosso meio ocorreu em 1997 16 , isolada de pescado, contudo, desde este período não há nenhum registro desse sorotipo envolvido em surto humano no Brasil.…”

Section: Resultsunclassified

Características de Vibrio parahaemolyticus isolados de mexilhões (Perna perna) comercializados em Niterói, Rio de Janeiro

Pereira

Possas

Viana

et al. 2007

Rev. Soc. Bras. Med. Trop.

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O ecossistema marinho é o habitat natural de bactérias como Vibrio parahaemolyticus, um importante patógeno causador de gastrenterite humana associada ao consumo de alimentos marinhos. Na presente investigação, foi avaliada a presença de V. parahaemolyticus a partir de 86 amostras de mexilhões in natura e pré-cozidos. Vibrio parahaemolyticus foi isolado a partir de 11,6% dos mexilhões in natura e pré-cozidos avaliados. Todas as cepas avaliadas demonstraram-se urease positivas e 28,5% Kanagawa positivas sugerindo um potencial patogênico para o homem. Houve a predominância do sorotipo O10:K52 e a identificação da cepa emergente O3:K6. Esses resultados apontam para a relevância epidemiológica de V. parahaemolyticus em casos de gastrenterite humana após consumo de mexilhões sem cozimento adequado (100ºC/15min). Além disso, é importante alertar as autoridades de Vigilância Sanitária no Brasil quanto a sua presença na cadeia alimentar e seus riscos para a Saúde Pública.

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Section: Resultsunclassified

Características de Vibrio parahaemolyticus isolados de mexilhões (Perna perna) comercializados em Niterói, Rio de Janeiro

Pereira

Possas

Viana

et al. 2007

Rev. Soc. Bras. Med. Trop.

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“…The flesh and intervalve liquid were aseptically placed in sterile bags, making up the pool of related to water temperature, with a higher concentration being present in oysters from warm water and outbreaks occurring usually during summer months (CABRERA-GARCIA; VASQUEZ-SALINAS; QUINONES-RAMIREZ, 2004;PEREIRA;VIANA;RODRIGUES, 2004;PEREIRA et al, 2007a;YOON et al, 2008). However, no close correlation has been noted between microorganisms of fecal origin and vibrios that are potentially pathogenic for humans (NORMANNO et al, 2006;OLIVER;WARNER;CLELAND, 1983).…”

Section: Introductionmentioning

confidence: 99%

Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil

et al. 2012

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Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil IntroductionIn Brazil, bivalve mollusks production takes place mainly in Santa Catarina State, south Brazil, due to the excellent geographical conditions of this area for marine organism cultures, such as the presence of a large number of bays facilitating the establishment of mollusk farms (COELHO et al., 2003;CORRÊA et al., 2007;OLIVEIRA NETO, 2005). In 2007, about 11.000 t of mollusks were commercialized in Santa Catarina State, the largest producing region of oysters (Crassostrea gigas). The major production takes place in sea farms located in the South Bay of Santa Catarina Island (OLIVEIRA NETO, 2007).Oysters are filter-feeders that efficiently concentrate microorganisms from polluted habitats, and because they are often consumed raw, they pose a health risk to consumers (CORRÊA et al., 2007;LALOO et al., 2000; PEREIRA et al., 2006;SILVA et al., 2004). The enteric bacteria, originating from the contamination of water with human residues, can readily contaminate the fauna in marine environments, especially molluscan shellfish (CORRÊA et al., 2007, PEREIRA et al., 2006. To guarantee sanitary quality, mollusk cultures should be monitored for contamination by pathogenic microorganisms (CORRÊA et al., 2007).Vibrios are very common in marine and estuarine water environments and some may cause infections in humans that were exposed to seafood or sea water. Several Vibrio species are pathogenic to humans and may be present in raw or partially cooked shellfish (LHAFI; KÜHNE, 2007; PEREIRA et al., 2007a, b , para ambos os microrganismos, sugerindo um monitoramento tanto destas espécies quanto da temperatura das águas marinhas nas regiões de cultivo. Com base nos resultados das análises microbiológicas, as amostras analisadas mostraram qualidade bacteriológica aceitável, ou seja, dentro dos parâmetros estabelecidos na legislação brasileira. Palavras-chave: qualidade microbiológica; moluscos bivalves; filtradores; Escherichia coli; Vibrio parahaemolyticus; Vibrio vulnificus. AbstractThe aim of this study was to assess the contamination of oysters (Crassostrea gigas), harvested in six different regions of the South Bay of Santa Catarina Island, with Coliforms at 45 °C, Escherichia coli, Vibrio spp., positive coagulase staphylococci, and Salmonella sp. over a period of one year. One hundred eighty oyster samples were collected directly from their culture sites and analyzed. Each sample consisted of a pool of 12 oysters. All of the samples analyzed showed absence of Salmonella, 18 (10%) samples showed presence of Escherichia coli, 15 (8.3%) samples were positive for V. alginolyticus, and Vibrio cholerae was detected in 4 samples (2.2%). The counts of positive-coagulase staphylococci varied from <10 to 1.9 × 10 2 CFU.g , for both microorganisms. This suggests the need for monitoring these vibrios contamination in oysters. Based on the results of the ...

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“…This is supported by Pereira et al 7 , according to whom prolonged storage of oysters at room temperature favors bacterial multiplication.…”

mentioning

confidence: 66%

“…The virulence factors were not expressed simultaneously in any strain. Similarly, Pereira et al 7 only observed ureolysis in Kanagawa-negative strains of V. parahaemolyticus.…”

Section: Conflict Of Interestmentioning

confidence: 93%

Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

Costa

Araújo

Vieira

2013

Rev. Soc. Bras. Med. Trop.

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Introduction:The present study aimed to survey the Vibrio microbiota of oysters (Crassostrea rhizophorae) obtained from restaurants in Fortaleza, State of Ceará, Brazil, and to identify virulence factors. Methods: The isolated vibrios were submitted to biochemical identification and were tested for hemolytic and urease activities. Results: The isolated strains belonged to 13 species, with predominance of Vibrio mimicus. Of the strain isolates only from fresh samples, 20.5% and 2.8% showed hemolytic and urease activities, respectively. Conclusions: The findings support the little-publicized claim that Vibrio species other than V. parahaemolyticus and V. vulnificus can represent a health risk to public health.Keywords: Vibrio. Crassostrea rhizophorae. Virulence factors.Vibrios are part of the native microbiota of marine environments and organisms. Up to 40% of water-column bacteria are associated with zooplankton. Among these, vibrios have a competitive advantage in the colonization of chitinous exoskeletons 1 . Due to their abundance in the water column, vibrios may be up to a hundred times more concentrated in filter-feeding mollusks than in the immediate environment 2 .Since vibrios are part of the autochthonous bacterial community colonizing bivalves, it comes as no surprise that they have been implicated in many outbreaks related to oyster consumption. The vibrio species most often associated with disease from consumption of oysters in natura are Vibrio parahaemolyticus, V. vulnificus, and V. cholerae 3 . However, virulence factors similar to those displayed by V. parahaemolyticus are detected in other vibrio species with increasing frequency. Thus, Nishibuchi et al. 4 found that V. hollisae strains from clinical samples can carry the hemolysinencoding gene tdh, a marker of virulence. Other vibrio species isolated from marine organisms have also been shown to be capable of β-hemolysis 5 .The objective of the present study was to survey the Vibrio microbiota of fresh and frozen oysters (Crassostrea rhizophorae) obtained from restaurants in Fortaleza, and to identify phenotypical virulence factors by testing strains for β-hemolysis and urease activity.The study was based on 15 samples of fresh oysters and 15 samples of frozen oysters (C. rhizophorae) obtained from two restaurants in Fortaleza City (Northeastern Brazil) between August 2009 and August 2010. Each sample consisted of 10 specimens, for a total of 300 specimens examined. Oysters with closed valves were placed in labeled polyethylene bags and transported in isothermal boxes to the Laboratory of Seafood and Environmental Microbiology (LABOMAR/UFC) for processing. Transportation did not exceed two hours.The oysters were washed in tap water and vigorously scrubbed, then opened under aseptic conditions for retrieval of the intervalvular tissues and fluid. A 50-gram portion was taken from each sample of 10 specimens and added to 450mL alkaline peptone water (pH 8.5) containing 1% NaCl. The sample was homogenized in a magnetic stirrer (Cole Parmer,...

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Vibrio parahaemolyticus produtores de urease isolados a partir de ostras (Crassostrea rizophorae) coletadas in natura em restaurantes e mexilhões (Perna perna) de banco natural

Cited by 15 publications

References 10 publications

Características de Vibrio parahaemolyticus isolados de mexilhões (Perna perna) comercializados em Niterói, Rio de Janeiro

Características de Vibrio parahaemolyticus isolados de mexilhões (Perna perna) comercializados em Niterói, Rio de Janeiro

Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil

Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

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