PPM1B and P-IKKβ expression levels correlated inversely with rat gastrocnemius atrophy after denervation

Wei, Jian Jun; Liang, Bing-sheng

doi:10.1590/s0100-879x2012007500080

Cited by 8 publications

(12 citation statements)

References 27 publications

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“…Wei and Liang [41] identified a negative correlation between PPM1B and muscle atrophy, that is, PPM1B expression gradually decreased when muscle atrophy increased.…”

Section: Discussionmentioning

confidence: 99%

Comparative muscle transcriptome associated with carcass traits of Nellore cattle

et al. 2017

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BackgroundCommercial cuts yield is an important trait for beef production, which affects the final value of the products, but its direct determination is a challenging procedure to be implemented in practice. The measurement of ribeye area (REA) and backfat thickness (BFT) can be used as indirect measures of meat yield. REA and BFT are important traits studied in beef cattle due to their strong implication in technological (carcass yield) and nutritional characteristics of meat products, like the degree of muscularity and total body fat. Thus, the aim of this work was to study the Longissimus dorsi muscle transcriptome of Nellore cattle, associated with REA and BFT, to find differentially expressed (DE) genes, metabolic pathways, and biological processes that may regulate these traits.ResultsBy comparing the gene expression level between groups with extreme genomic estimated breeding values (GEBV), 101 DE genes for REA and 18 for BFT (false discovery rate, FDR 10%) were identified. Functional enrichment analysis for REA identified two KEGG pathways, MAPK (Mitogen-Activated Protein Kinase) signaling pathway and endocytosis pathway, and three biological processes, response to endoplasmic reticulum stress, cellular protein modification process, and macromolecule modification. The MAPK pathway is responsible for fundamental cellular processes, such as growth, differentiation, and hypertrophy. For BFT, 18 biological processes were found to be altered and grouped into 8 clusters of semantically similar terms. The DE genes identified in the biological processes for BFT were ACHE, SRD5A1, RSAD2 and RSPO3. RSAD2 has been previously shown to be associated with lipid droplet content and lipid biosynthesis.ConclusionIn this study, we identified genes, metabolic pathways, and biological processes, involved in differentiation, proliferation, protein turnover, hypertrophy, as well as adipogenesis and lipid biosynthesis related to REA and BFT. These results enlighten some of the molecular processes involved in muscle and fat deposition, which are economically important carcass traits for beef production.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3897-x) contains supplementary material, which is available to authorized users.

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“…Wei and Liang [41] identified a negative correlation between PPM1B and muscle atrophy, that is, PPM1B expression gradually decreased when muscle atrophy increased.…”

Section: Discussionmentioning

confidence: 99%

Comparative muscle transcriptome associated with carcass traits of Nellore cattle

et al. 2017

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“…The present study shows that p-IKKb and NF-jB p65 activity are higher in the PVN of salt-induced hypertensive rats. It is consisted of studies from others and our previous work [7,30,31]. The maturation of IL-1b is dependent on NLRP3 inflammasome assembling and the following activation [9,32,33].…”

Section: Discussionmentioning

confidence: 99%

NF-κB Blockade in Hypothalamic Paraventricular Nucleus Inhibits High-Salt-Induced Hypertension Through NLRP3 and Caspase-1

Shi

et al. 2015

Cardiovasc Toxicol

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High-salt-induced inflammation and oxidative stress in the hypothalamic paraventricular nucleus (PVN) contribute to the pathogenesis of salt-sensitive hypertension. In this study, we hypothesized that chronic inhibition of nuclear factor-κB (NF-κB) activity in the PVN delays the progression of hypertension by upregulating anti-inflammatory cytokines, reducing NLRP3 (NOD-like receptor family pyrin domain containing 3) and IL-1β and attenuating p-IKKβ, NF-κB p65 activity and NAD(P)H oxidase in the PVN of salt-sensitive hypertensive rats. Dahl salt-sensitive rats received a high-salt diet (HS, 8 % NaCl) or a normal-salt diet (NS, 0.3 % NaCl) for 6 weeks and were treated with bilateral PVN infusion with either vehicle or pyrrolidine dithiocarbamate (PDTC, 5 μg/h), a NF-κB inhibitor via osmotic minipump. The mean arterial pressure and plasma levels of norepinephrine (NE) and epinephrine (EPI) were significantly increased in high-salt-fed rats. In addition, rats with high-salt diet had higher levels of p-IKKβ, NF-κB p65 activity, Fra-like (Fra-LI) activity (an indicator of chronic neuronal activation), NOX-4 (subunits of NAD(P)H oxidase), NLRP3 and IL-1β, and lower levels of IL-10 in the PVN than normal diet rats. Bilateral PVN infusions of PDTC attenuated these high-salt-induced changes. These findings suggest that high-salt-induced NF-κB activation in the PVN caused hypertension via sympathoexcitation, which are associated with the increases of NLRP3, IL-1β and oxidative stress in the PVN; PVN inhibition of NF-κB activity attenuates NLRP3, IL-1β and oxidative stress in the PVN and thereby attenuates hypertension.

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“…Protein phosphatase 1B (PPM1B) is a member of PPM superfamily that are Mg 2+ /Mn 2+ ‐dependent monomeric phosphatases 9 . It has been demonstrated to be involved in several signalling pathways depending on the cellular context, such as MAPK signalling, 10 AMPK signalling, 11 TNF signalling, 12,13 gene transcription 14 and cell cycle 15 . However, its physiological functions and the biological relevance in the human diseases are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Identification of HN252 as a potent inhibitor of protein phosphatase PPM1B

Peng

Zhou

et al. 2020

J Cellular Molecular Medi

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Protein phosphatase 1B (PPM1B), a member of metal‐dependent protein serine/threonine phosphatase family, is involved in the regulation of several signalling pathways. However, our understanding of its substrate interaction and physiological functions is still largely limited. There is no reported PPM1B inhibitor to date. In this study, we identified HN252, a p‐terphenyl derivative, as a potent PPM1B inhibitor (Ki = 0.52 ± 0.06 µM). HN252 binding to PPM1B displayed remarkable and specific inhibition of PPM1B in both in vitro and ex vivo. With the aid of this small molecular inhibitor, we identified 30 proteins’ serine/threonine phosphorylation as potential substrates of PPM1B, 5 of which were demonstrated by immunoprecipitation, including one known (CDK2) and 4 novel ones (AKT1, HSP90B, β‐catenin and BRCA1). Furthermore, GO and KEGG analysis of dramatically phosphorylated proteins by PPM1B inhibition indicated that PPM1B plays roles in the regulation of multiple cellular processes and signalling pathways, such as gene transcription, inflammatory regulation, ageing and tumorigenesis. Our work provides novel insights into further investigation of molecular mechanisms of PPM1B.

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PPM1B and P-IKKβ expression levels correlated inversely with rat gastrocnemius atrophy after denervation

Cited by 8 publications

References 27 publications

Comparative muscle transcriptome associated with carcass traits of Nellore cattle

Comparative muscle transcriptome associated with carcass traits of Nellore cattle

NF-κB Blockade in Hypothalamic Paraventricular Nucleus Inhibits High-Salt-Induced Hypertension Through NLRP3 and Caspase-1

Identification of HN252 as a potent inhibitor of protein phosphatase PPM1B

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