“…However, this anxiolytic-like response is lost after the injection of higher AEA doses, characterizing an inverted U-shaped curve [20,29]. This finding agrees with other observations indicating that the emotional effects of cannabinoids compounds occur in a biphasic dose-dependent way, inducing anxiolytic-like effects at lower doses and no response, or even an anxiogenic one, at higher doses [5,12,15,17,19,33]. This profile could be explained by the capacity of cannabinoids to bind to different classes of receptors, such as CB 1 and TRPV 1 , and influencing the release of neurotransmitters such as GABA, glutamate and NO, which may have distinct roles in the modulation emotional responses [14,16,33,34,35].…”