Viability of mesenchymal stem cells during electrospinning

Zanatta, Geancarlo; Steffens, Daniela; Braghirolli, Daikelly Iglesias; Fernandes, Raquel Arrieche; Netto, Carlos Alexandre; Pranke, Patrícia

doi:10.1590/s0100-879x2011007500163

Cited by 19 publications

(13 citation statements)

References 31 publications

(39 reference statements)

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“…The scaffold mimics the ECM in providing support and physical attachments for the spheroids. The nano- and micro-topographic and mechanotransductive cues of electrospun polymeric scaffolds [29]–[35] have been reported to stimulate migration, differentiation and gene expression of cancer cells [36]–[38]. The electrospinning conditions can be adjusted to produce fibrous scaffolds tailored for specific cell culture needs [35], [39].…”

Section: Introductionmentioning

confidence: 99%

A 3D Fibrous Scaffold Inducing Tumoroids: A Platform for Anticancer Drug Development

et al. 2013

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The development of a suitable three dimensional (3D) culture system for anticancer drug development remains an unmet need. Despite progress, a simple, rapid, scalable and inexpensive 3D-tumor model that recapitulates in vivo tumorigenesis is lacking. Herein, we report on the development and characterization of a 3D nanofibrous scaffold produced by electrospinning a mixture of poly(lactic-co-glycolic acid) (PLGA) and a block copolymer of polylactic acid (PLA) and mono-methoxypolyethylene glycol (mPEG) designated as 3P. Cancer cells cultured on the 3P scaffold formed tight irregular aggregates similar to in vivo tumors, referred to as tumoroids that depended on the topography and net charge of the scaffold. 3P scaffolds induced tumor cells to undergo the epithelial-to-mesenchymal transition (EMT) as demonstrated by up-regulation of vimentin and loss of E-cadherin expression. 3P tumoroids showed higher resistance to anticancer drugs than the same tumor cells grown as monolayers. Inhibition of ERK and PI3K signal pathways prevented EMT and reduced tumoroid formation, diameter and number. Fine needle aspirates, collected from tumor cells implanted in mice when cultured on 3P scaffolds formed tumoroids, but showed decreased sensitivity to anticancer drugs, compared to tumoroids formed by direct seeding. These results show that 3P scaffolds provide an excellent platform for producing tumoroids from tumor cell lines and from biopsies and that the platform can be used to culture patient biopsies, test for anticancer compounds and tailor a personalized cancer treatment.

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Section: Introductionmentioning

confidence: 99%

A 3D Fibrous Scaffold Inducing Tumoroids: A Platform for Anticancer Drug Development

et al. 2013

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“…16 Recent studies have shown that microfiber scaffolds can be good substrates for cell growth and favor the adhesion and spreading of cells on their surface. [17][18][19] Furthermore, the pore size is directly related to the size of the fiber diameter in scaffolds produced by electrospinning. 20 Thus, the SCCs probably exhibited favorable conditions for the development of cells within their structures, offering space for cellular growth and facilitating the transportation of nutrition and exchange of metabolic substances.…”

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confidence: 99%

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Braghirolli¹,

Zamboni²,

Acasigua³

et al. 2015

IJN

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In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide- co -glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO 2 . The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

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“…Similar results for proliferation but without evaluating differentiation after incorporation in a PLGA scaffold were found by Braghirolli et al 53 . A different approach evaluated by Zanatta et al 50 was a monoaxial spinning and spraying process using a water-soluble polymer (PVA -polyvinyl alcohol) and MSCs or Mononuclear cells (MNCs). This approach resulted in unsatisfactory cellular survival of 19,6% using MSCs and 8,38% using MNCs, thus indicating that the separation of polymer and cells in a coaxial process is the most promising approach.…”

Section: Discussionmentioning

confidence: 99%

Direct incorporation of mesenchymal stem cells into a Nanofiber scaffold – in vitro and in vivo analysis

Schüttler

Bauhofer

Ketter

et al. 2020

Sci Rep

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Bony defects are a common problem in musculoskeletal surgery. Replacement with autologous bone grafts is limited by availability of transplant material. Sterilized cancellous bone, while being osteoconductive, has limited osteoinductivity. Nanofiber scaffolds are currently used for several purposes due to their capability of imitating the extracellular matrix. Furthermore, they allow modification to provide functional properties. Previously we showed that electrospun nanofiber scaffolds can be used for bone tissue regeneration. While aiming to use the osteoinductive capacities of collagen type-I nanofibers we saw reduced scaffold pore sizes that limited cellular migration and thus colonization of the scaffolds. Aim of the present study was the incorporation of mesenchymal stem cells into the electrospinning process of a nanofiber scaffold to produce cell-seeded nanofiber scaffolds for bone replacement. After construction of a suitable spinning apparatus for simultaneous electrospinning and spraying with independently controllable spinning and spraying devices and extensive optimization of the spinning process, in vitro and in vivo evaluation of the resulting scaffolds was conducted. Stem cells isolated from rat femora were incorporated into PLLA (poly-l-lactide acid) and PLLA-collagen type-I nanofiber scaffolds (PLLA Col I Blend) via simultaneous electrospinning and-spraying. Metabolic activity, proliferation and osteoblastic differentiation were assessed in vitro. for in vivo evaluation scaffolds were implanted into critical size defects of the rat scull. After 4 weeks, animals were sacrificed and bone healing was analyzed using CT-scans, histological, immunhistochemical and fluorescence evaluation. Successful integration of mesenchymal stem cells into the scaffolds was achieved by iteration of spinning and spraying conditions regarding polymer solvent, spinning distance, the use of a liquid counter-electrode, electrode voltage and spinning duration. In vivo formation of bone tissue was achieved. Using a PLLA scaffold, comparable results for the cell-free and cell-seeded scaffolds were found, while the cell-seeded PLLA-collagen scaffolds showed significantly better bone formation when compared to the cell-free PLLA-collagen scaffolds. These results provide support for the future use of cell-seeded nanofiber scaffolds for large bony defects. Bony defects are a common problem in musculoskeletal surgery e.g. after tumor resection, infection, revision arthroplasty or trauma. In general, studies have shown that the physiological process of fracture healing is disturbed in 5-30% which results in delayed or incomplete recovery, depending on the treatment 1. To treat such defects, adequate bone replacement is needed. As this affects approximately 500,000 surgeries in Europe and approximately 2.2 million surgeries per year worldwide, bone is the second most transplanted tissue 2,3. Although replacement with autologous bone is the "golden standard", this procedure is limited by availability of transplant material and ster...

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Viability of mesenchymal stem cells during electrospinning

Cited by 19 publications

References 31 publications

A 3D Fibrous Scaffold Inducing Tumoroids: A Platform for Anticancer Drug Development

A 3D Fibrous Scaffold Inducing Tumoroids: A Platform for Anticancer Drug Development

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Direct incorporation of mesenchymal stem cells into a Nanofiber scaffold – in vitro and in vivo analysis

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