Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil

Pisa, Giovani; Magnani, Galileo; Weber, Heroldo; Souza, Emanuel M.; Faoro, Helisson; Monteiro, Rose A.; Daros, Edelclaiton; Baura, Valter A.; Bespalhok, João; Pedrosa, Fábio O.; Cruz, Leonardo M.

doi:10.1590/s0100-879x2011007500148

Cited by 56 publications

(44 citation statements)

References 29 publications

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“…This crop has great importance for Brazilian economy as a commodity for exportation, but also as a major inducer of technological development (Pisa et al, 2011). Nowadays, Brazil is the leader in sugarcane production with assets of 10 million hectares and an average yield around 74 Mg/ha (IBGE, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…There is a high diversity of PGPB associated with the rhizosphere and shoot of sugarcane, and the knowledge of the diversity of PGPB associated with sugarcane is an important step to prospect bacteria responsible for promoting BNF and plant growth, and consequently, improving the crop productivity (Pisa et al, 2011). On the hand, the number of species associated with the rhizosphere of sugarcane and identified as PGPB has increased considerably in recent years due to the development of molecular tools (Chauhan et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of plant growth-promoting bacteria associated with sugarcane

Antunes¹,

Lyra²,

Ollero³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potential inoculant to the crop. We evaluated the genetic diversity of PGPB in the plant tissue of sugarcane varieties (RB 867515, RB 1011, and RB 92579). The primer BOX-A1R was used to differentiate the similar isolated and further sequencing 16S rRNA ribosomal gene. The 16S rRNA gene showed the presence of seven different genera distributed into four groups, the genus Bacillus, followed by Paenibacillus (20%), Burkholderia (14%), Herbaspirillum (6%), Pseudomonas (6%), Methylobacterium (6%), and Brevibacillus (3%). The molecular characterization of endophytic isolates from sugarcane revealed a diversity of bacteria colonizing this plant, with a possible biotechnological potential to be used as inoculant and biofertilizers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of plant growth-promoting bacteria associated with sugarcane

Antunes¹,

Lyra²,

Ollero³

et al. 2017

Genet. Mol. Res.

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“…To assess the diversity and taxonomy of crop plant-associated bacteria, many cultivation-dependent and -independent methods are currently in use (14)(15)(16). However, most of these methods are not quantitative and are based on the evaluation of the 16S rRNA gene coding sequences.…”

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confidence: 99%

Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

Stets

Alquéres

Souza

et al. 2015

Appl Environ Microbiol

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bAzospirillum is a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide. Azospirillum brasilense strains exhibit a growth-promoting effect by means of phytohormone production and possibly by N 2 fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification of A. brasilense FP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences of A. brasilense FP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for the A. brasilense FP2 strain. These primer pairs were used to monitor quantitatively the population of A. brasilense in wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed that A. brasilense FP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (ϳ10 7 CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.A zospirillum is one of the most important genera of plant growth-promoting rhizobacteria found worldwide under a variety of environmental and soil conditions (1). The diazotroph Azospirillum brasilense is the best-studied species of the genus, is found in close association with many agriculturally important crops, and exerts beneficial effects on plant growth and productivity (2-4). Nitrogen fixation (5, 6) and the production of the auxin 3-indoleacetic acid (IAA) by many representatives of the genus Azospirillum are related to the growth promotion effects observed in inoculated plants, such as increases in root length and the numbers of root hairs and lateral roots (3).The biotechnological use of A. brasilense inoculants in Latin American and in Brazil, in particular, has increased in recent years (7). Strain FP2 is a spontaneous mutant of A. brasilense Sp7 (8). Strain Sp7 has been shown to be capable of stimulating the growth of several members of the family Poaceae and increasing the productivities of wheat and maize crops (2). Strain FP2 can also promote the growth of wheat (9) and enhance maize and wheat productivity under field conditions (unpublished data). Most of the A. brasilense inoculants in B...

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“…msu.edu/html/). Pisa et al (2011) used culture independent approach for the evaluation of sugarcane soil microflora under different nitrogen fertilization regimes. Based on the sequencing results, these authors reported dominance of different phylum in the ascending order as Proteobacteria (29.6%), Acidobacteria (23.4%), Actinobacteria (5.6%), Bacteroidetes (12.1%), and Firmicutes (10.2%).…”

Section: Substrate Induced Respiration Technique (Sir)mentioning

confidence: 99%