Dynamics of immunosuppression in hamsters with experimental visceral leishmaniasis

Fazzani, C.; Guedes, P.A.; Senna, Ana J.S.; Souza, Errol B. De; Goto, Hiro; Lindoso, José Ângelo Lauletta

doi:10.1590/s0100-879x2011007500062

Cited by 4 publications

(2 citation statements)

References 15 publications

(13 reference statements)

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“…In VL, it is widely recognized that mononuclear cells have low, or even absent, proliferative potential to parasite antigens, indicating the immunosuppressive status of the cellular immune response (Fazzani et al., 2011). Therefore, the ability of mononuclear cells to proliferate under specific antigens suggests that the animals in the present study developed a beneficial immune response following treatment.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Jesus

Fragoso

Yamamoto

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

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Leishmaniasis is an important neglected tropical disease, affecting more than 12 million people worldwide. The available treatments are not well tolerated and present diverse side effects in patients, justifying the search for new therapeutic compounds. In the present study, the therapeutic potential and toxicity of ursolic acid (UA), isolated from the leaves of Baccharis uncinella C. DC. (Asteraceae), were evaluated in experimental visceral leishmaniasis. To evaluate the therapeutic potential of UA, hamsters infected with L. (L.) infantum were treated daily during 15 days with 1.0 or 2.0 mg UA/kg body weight, or with 5.0 mg amphotericin B/kg body weight by intraperitoneal route. Fifteen days after the last dose, the parasitism of the spleen and liver was stimated and the main histopathological alterations were recorded. The proliferation of splenic mononuclear cells was evaluated and IFN-γ, IL-4, and IL-10 gene expressions were analyzed in spleen fragments. The toxicity of UA and amphotericin B were evaluated in healthy golden hamsters by histological analysis and biochemical parameters. Animals treated with UA had less parasites in the spleen and liver when compared with the infected control group, and they also showed preservation of white and red pulps, which correlate with a high rate of proliferation of splenic mononuclear cells, IFN-γ mRNA and iNOS production. Moreover, animals treated with UA did not present alterations in the levels of AST, ALT, creatinine and urea. Taken together, these findings indicate that UA is an interesting natural compound that should be considered for the development of prototype drugs against visceral leishmaniasis.

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Section: Discussionmentioning

confidence: 99%

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Jesus

Fragoso

Yamamoto

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

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“…The latter dysfunction has been attributed to the inability of the infected antigen-presenting cells (APCs) to stimulate specific T cells, the production of TGF-β which triggers the apoptotic death of lymphocytes and the downregulation of protein kinase C activity 9,72,95 . Interestingly, the antigen-dependent immunosuppression observed in L. chagasi -infected hamsters with active visceral disease is not related to the cytokine profile 41 .…”

Section: Hamster Modelmentioning

confidence: 86%

Animal Models for the Study of Leishmaniasis Immunology

Loría-Cervera

Andrade-Narvaez

2014

Rev. Inst. Med. trop. S. Paulo

131

101

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Leishmaniasis remains a major public health problem worldwide and is classified as Category I by the TDR/WHO, mainly due to the absence of control. Many experimental models like rodents, dogs and monkeys have been developed, each with specific features, in order to characterize the immune response to Leishmania species, but none reproduces the pathology observed in human disease. Conflicting data may arise in part because different parasite strains or species are being examined, different tissue targets (mice footpad, ear, or base of tail) are being infected, and different numbers (“low” 1×102 and “high” 1×106) of metacyclic promastigotes have been inoculated. Recently, new approaches have been proposed to provide more meaningful data regarding the host response and pathogenesis that parallels human disease. The use of sand fly saliva and low numbers of parasites in experimental infections has led to mimic natural transmission and find new molecules and immune mechanisms which should be considered when designing vaccines and control strategies. Moreover, the use of wild rodents as experimental models has been proposed as a good alternative for studying the host-pathogen relationships and for testing candidate vaccines. To date, using natural reservoirs to study Leishmania infection has been challenging because immunologic reagents for use in wild rodents are lacking. This review discusses the principal immunological findings against Leishmania infection in different animal models highlighting the importance of using experimental conditions similar to natural transmission and reservoir species as experimental models to study the immunopathology of the disease.

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Immunological determinants, host immune responses, and animal models of visceral leishmaniasis

Kumar

2021

Pathogenesis, Treatment and Prevention of Leishmaniasis

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Dynamics of immunosuppression in hamsters with experimental visceral leishmaniasis

Cited by 4 publications

References 15 publications

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Animal Models for the Study of Leishmaniasis Immunology

Immunological determinants, host immune responses, and animal models of visceral leishmaniasis

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