Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D

Diniz, Mariana O.; Ferreira, Luís Carlos de Souza

doi:10.1590/s0100-879x2011007500039

Cited by 13 publications

(8 citation statements)

References 36 publications

(17 reference statements)

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“…Cartridges were prepared prior to the day of the experiments, followed by general methods [31]. First, the amount of DNA and gold required for each transformation was calculated.…”

Section: Methodsmentioning

confidence: 99%

Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase ofSchistosoma japonicum

Cao

Zhao

Han

et al. 2013

BioMed Research International

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Schistosomiasis, caused by infection with Schistosoma species, remains an important parasitic zoonosis. Thioredoxin glutathione reductase of Schistosoma japonicum (SjTGR) plays an important role in the development of the parasite and for its survival. Here we present a recombinant plasmid DNA vaccine, pVAX1/SjTGR, to estimate its protection against S. japonicum in BALB/c mice. The DNA vaccine administrated by particle bombardment induced higher protection than by intramuscular injection. All animals vaccinated with pVAX1/SjTGR developed significant specific anti-SjTGR antibodies than control groups. Moreover, animals immunized by gene gun exhibited a splenocyte proliferative response, with an increase in IFN-γ and IL-4. The recombinant plasmid administrated by gene gun achieved a medium protective efficacy of 27.83–38.83% (P < 0.01) of worm reduction and 40.38–44.51% (P < 0.01) of liver egg count reduction. It suggests that different modes of administering a DNA vaccine can influence the protective efficacy induced by the vaccine. Interestingly, from the enzymatic activity results, we found that worms obtained from pVAX1/SjTGR-vaccinated animals expressed lower enzymatic activity than the control group and the antibodies weakened the enzymatic activity of SjTGR in vitro, too. It implies that the high-level antibodies may contribute to the protective effects.

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“…Cartridges were prepared prior to the day of the experiments, followed by general methods [31]. First, the amount of DNA and gold required for each transformation was calculated.…”

Section: Methodsmentioning

confidence: 99%

Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase ofSchistosoma japonicum

Cao

Zhao

Han

et al. 2013

BioMed Research International

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“…Significantly stronger therapeutic antitumor effects were observed after i.d. immunization with a single dose of pgD-E7E6E5 co-administration of DNA vectors encoding a co-stimulatory signal, such as granulocyte-macrophage colony-stimulating factor (GM-CSF) or IL-12 [50].…”

Section: Recently Applied Therapeutic Vaccine Strategiesmentioning

confidence: 99%

New Developments in Therapeutic HPV Vaccines

Lin

Albers

et al. 2012

Curr Obstet Gynecol Rep

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Human papillomavirus (HPV) infection is the central causal factor in cervical cancer. For years efforts were undertaken to control HPV-associated disease by the development of HPV vaccines. While prophylactic HPV vaccination represents a major public health breakthrough, it is limited to new infection by certain HPV types and does not have therapeutic effects or prevent progression of preexisting HPV infections to malignancy. Thus, there is an urgent need for therapeutic HPV vaccines. Therapeutic vaccines aim to induce cytotoxic T cells, facilitating clearance of HPV high-grade lesions and cancers. HPV E6 and E7 oncogenes represent ideal antigens for therapeutic HPV vaccination because of their constitutive expression in HPV-containing carcinomas and their critical role in the induction and maintenance of HPV-associated disease. This review discusses the most recent advances in HPV therapeutic vaccines and summarizes effects of new basic immunology findings and vaccine delivery technologies.

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“…Os desafios incluem o estado dos pacientes imunocomprometidos, a dificuldade em estimular o sistema imunológico, os mecanismos de escape imunológico utilizado por tumores e células infectadas pelo vírus, e as preocupações de segurança. Apesar disso, há muitos resultados encorajadores sugerindo que as respostas de células T às proteínas E6 e E7 podem ser geradas em modelos animais como em seres humanos (Mahdavi, Monk, 2005 (Diniz, Ferreira, 2011;Frazer, 2004;Govan, 2005;Jochmus et al, 1999;Lasaro et al, 2005;Mahdavi, Monk, 2005;Padilla-Paz, 2005). …”

Section: Vacina Terapêutica Contra Lesões Intraepiteliais E Câncer Ceunclassified

“…Algumas dessas estratégias consistem de vacina de DNA expressando os antígenos E6 e E7 fusionados a uma proteína capaz de aumentar a resposta citotóxica contra o tumor, os exemplos incluem proteína do choque térmico (HSPs) (Chen et al, 2000;Huang et al, 2007), calreticulina (Kim et al, 2008), fragmento C da toxina tetânica (Oosterhuis et al, 2012), glicoproteína gD do vírus da herpes (Diniz, Ferreira, 2011) e a proteína Ubiquitina.…”

Section: Vários Estudos Demonstraram Que O Proteassomo Principalmentunclassified

Avaliação imunológica da vacina proteica E6E7 fusionada a Ubiquitina contra cancer cervical induzido por HPV16.

Oliveira¹

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Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D

Cited by 13 publications

References 36 publications

Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase ofSchistosoma japonicum

Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase ofSchistosoma japonicum

New Developments in Therapeutic HPV Vaccines

Avaliação imunológica da vacina proteica E6E7 fusionada a Ubiquitina contra cancer cervical induzido por HPV16.

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