5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae)

Almeida, Jorge Rady de; Sauaia, H.; Viana, Jaciara

doi:10.1590/s0100-879x2010007500126

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…However, increases in cell proliferation rates as a result of BrdU labeling (as suggested by Maldonado 2015) have not been reported in the literature nor supported by any data we are aware of. In contrast, although BrdU can be toxic in high doses (at least three orders of magnitude higher than applied in the cell proliferation studies described above), its toxicity has been found to be related to delayed cell division by lengthening the cell cycle and the inability of cells to divide (e.g., Taupin 2007;De Almeida et al 2010;Duque and Rakic 2011). Furthermore, several additional studies have shown choanocyte proliferation using different labeling techniques, i.e., titrated thymidine (Shore 1971;Efremova and Efremov 1979) and 5-ethynyl-2′-deoxyuridine (EdU) (Kahn and Leys 2016).…”

Section: Sponge Loop Controversies: Sponge Growth Cell Turnover and Detritus Productionmentioning

confidence: 99%

Nutrient Fluxes and Ecological Functions of Coral Reef Sponges in a Changing Ocean

Goeij

Lesser

Pawlik

2017

Climate Change, Ocean Acidification and Sponges

132

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Coral reefs are iconic examples of biological hotspots, highly appreciated because of their ecosystem services. Yet, they are threatened by human impact and climate change, highlighting the need to develop tools and strategies to curtail changes in these ecosystems. Remarkably, ever since Darwin's descriptions of coral reefs, it has been a mystery how one of Earth's most productive and diverse ecosystems thrives in oligotrophic seas, as an oasis in a marine desert. Sponges are now increasingly recognized as key ecosystem engineers, efficiently retaining and transferring energy and nutrients on the reef. As a result, current reef food web models, lacking sponge-driven resource cycling, are incomplete and need to be redeveloped. However, mechanisms that determine the capacity of sponge "engines," how they are fueled, and drive communities are unknown. Here we will discuss how sponges integrate within the novel reef food web framework. To this end, sponges will be evaluated on functional traits (morphology, associated microbes, pumping rate) in the processing of dissolved and particulate food. At the community level, we discuss to what extent these different traits are a driving force in structuring shallow-to deep-sea reef ecosystems, from fuel input (primary producers) to engine output (driving and modulating the consumer food web). Finally, as climate change causes the onset of alterations in the community structure and food web of reef ecosystems, there is evidence accumulating that certain biological pathways are triggered, such as the sponge loop and the microbial loop, that may shift reef ecosystems faster than their original stressors (e.g., warming oceans and ocean acidification). Unfortunately,

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Section: Sponge Loop Controversies: Sponge Growth Cell Turnover and Detritus Productionmentioning

confidence: 99%

Nutrient Fluxes and Ecological Functions of Coral Reef Sponges in a Changing Ocean

Goeij

Lesser

Pawlik

2017

Climate Change, Ocean Acidification and Sponges

132

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“…hygida. By using a combination of cytological analysis and larval injection of drugs that either inhibit DNA synthesis or chemically modify DNA and radiolabeled precursors, his group confirmed that sciarid DNA puffs are sites of developmentally regulated gene amplification and abundant RNA synthesis [18,20,[30][31][32]. Subsequent studies showed that the regulated synthesis of groups of salivary gland proteins was related to the expansion and regression of specific DNA puffs [33,34].…”

Section: Introductionmentioning

confidence: 99%

Pseudolycoriella hygida (Sauaia and Alves)—An Overview of a Model Organism in Genetics, with New Aspects in Morphology and Systematics

Menzel,

Kramp,

Amorim

et al. 2024

Insects

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Pseudolycoriella hygida (Sauaia & Alves, 1968) is a sciarid that has been continuously cultured in the laboratory for nearly 60 years. Studies on this species have contributed to the understanding of DNA puffs, which are characteristic of Sciaridae, and to the knowledge of more general aspects of insect biology, including cell death, nucleolar organization, and the role of the hormone ecdysone during molting. The genome of Psl. hygida has now been sequenced, and it is the third publicly available sciarid genome. The aim of this work is to expand the current knowledge on Psl. hygida. The morphology of the adults is revisited. The morphology of larvae and pupae is described, together with the behavior of immature stages under laboratory conditions. Cytogenetic maps of the salivary gland polytene chromosomes are presented, together with a comparative analysis of the mitotic chromosomes of six different sciarid species. Pseudolycoriella hygida was originally described as a species of Bradysia and recently moved to Pseudolycoriella. We examine here the systematic position of Psl. hygida in the latter genus. Our results extend the characterization of an unconventional model organism and constitute an important resource for those working on the cytogenetics, ecology, taxonomy, and phylogenetic systematics of sciarids.

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“…In addition, BrdU triggers cell death and the formation of teratomas, alters DNA stability, lengthens cell cycle, and has mitogenic, transcriptional, and translational effects on the cells that incorporate it (reviewed in [ 17 ]). The delaying effect of BrdU on DNA synthesis and cell division is probably caused by structural changes in the DNA helixes, which occur when a large amount of BrdU is incorporated into both strands of the DNA [ 18 ]. Furthermore, BrdU has been found to cause abnormal proliferation in vivo [ 19 ], although others found no cytotoxic effects on adult neurogenesis [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

The Cell Birth Marker BrdU Does Not Affect Recruitment of Subsequent Cell Divisions in the Adult Avian Brain

Cattan

Ayali

Barnea

2015

BioMed Research International

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BrdU is commonly used to quantify neurogenesis but also causes mutation and has mitogenic, transcriptional, and translational effects. In mammalian studies, attention had been given to its dosage, but in birds such examination was not conducted. Our previous study suggested that BrdU might affect subsequent cell divisions and neuronal recruitment in the brain. Furthermore, this effect seemed to increase with time from treatment. Accordingly, we examined whether BrdU might alter neurogenesis in the adult avian brain. We compared recruitment of [3H]-thymidine+ neurons in brains of zebra finches (Taeniopygia guttata) when no BrdU was involved and when BrdU was given 1 or 3 months prior to [3H]-thymidine. In nidopallium caudale, HVC, and hippocampus, no differences were found between groups in densities and percentages of [3H]-thymidine+ neurons. The number of silver grains per [3H]-thymidine+ neuronal nucleus and their distribution were similar across groups. Additionally, time did not affect the results. The results indicate that the commonly used dosage of BrdU in birds has no long-term effects on subsequent cell divisions and neuronal recruitment. This conclusion is also important in neuronal replacement experiments, where BrdU and another cell birth marker are given, with relatively long intervals between them.

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5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae)

Cited by 3 publications

References 36 publications

Nutrient Fluxes and Ecological Functions of Coral Reef Sponges in a Changing Ocean

Nutrient Fluxes and Ecological Functions of Coral Reef Sponges in a Changing Ocean

Pseudolycoriella hygida (Sauaia and Alves)—An Overview of a Model Organism in Genetics, with New Aspects in Morphology and Systematics

The Cell Birth Marker BrdU Does Not Affect Recruitment of Subsequent Cell Divisions in the Adult Avian Brain

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