Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood

Gomes, Natália E.; Brunialti, Milena Karina Coló; Mendes, Marialice Erdelyi; Freudenberg, Marina A.; Galanos, Chris; Salomão, Reinaldo

doi:10.1590/s0100-879x2010007500078

Cited by 61 publications

(54 citation statements)

References 34 publications

(24 reference statements)

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“…We have also shown that MTs are required to deliver CR3 receptors to the cell surface in classically activated macrophages (19). Here, we observe an up-regulation of TLR4 on the cell surface in activated macrophages, which is in agreement with studies on human monocytes (82). We believe that this is an MT-dependent process because of the following: (a) stathmin-GFP overexpression and concomitant MT disruption reduce surface TLR4 levels, and (b) total TLR4 levels are unaltered after macrophage activation ruling out a biosynthetic contribution to elevated TLR4 on the plasma membrane.…”

Section: Discussionsupporting

confidence: 91%

Down-regulation of Stathmin Is Required for the Phenotypic Changes and Classical Activation of Macrophages

Harrison

2015

Journal of Biological Chemistry

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Background: Macrophage activation involves pronounced microtubule (MT) stabilization. Results: Stathmin is degraded to enhance MT stabilization and allow robust activation in IFN␥-LPS-stimulated macrophages. Conclusion: MT stabilization by reduced stathmin levels is required for enhanced phagocytosis, signal transduction, and activation of macrophages. Significance: Understanding the nature and mechanisms of MT stabilization in activated macrophages is important for both immunology and cell biology fields.

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Section: Discussionsupporting

confidence: 91%

Down-regulation of Stathmin Is Required for the Phenotypic Changes and Classical Activation of Macrophages

Harrison

2015

Journal of Biological Chemistry

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“…They reported just kinetic differences in the action of rough LPS and smooth LPS: rough LPS acts more rapidly www.intechopen.com than S-LPS to cause functional alterations in neutrophils. Similar results were obtained on neutrophils in whole blood: again just kinetic difference was observed between R-and S-LPS in the expression of cell surface receptors CD11b and CD11c on neutrophils (Gomes et al, 2010). Nevetheless, the rough mutant as well as S LPS differ in some distinct physicochemical properties.…”

Section: Introductionsupporting

confidence: 77%

Neutrophil Cellular Responses to Various Salmonella typhimurium LPS Chemotypes

Zagryazhskaya¹,

Galkina²,

Grishina³

et al. 2012

Salmonella - A Diversified Superbug

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“…This could be due to a reduction of food consumption caused by higher secretion of pro-inflammatory cytokines (Bauer et al ., 2011;Parra et al ., 2013). It may also suggest that D3 results are related to a saturation of the recognition receptors that mediate cellular signaling induced by LPS (Gomes et al ., 2010).…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Zapata

Rodríguez²,

Ramírez³

et al. 2015

Rev Colomb Cienc Pecu

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El lipopolisacárido de Escherichia coli afecta la secreción de mucinas intestinales en cerdos destetados O lipopolissacárido de Escherichia coli afecta a secreção de mucina intestinal em porcos desmamadosSummary Background: to the best of our knowledge, the effects of lipopolysaccharide (LPS) from Escherichia coli on goblet cells and intestinal mucin secretion of weaned pigs has not been reported, and it is unknown whether these effects could trigger enteritis. Objective: to determine the effect of E . coli LPS on intestinal mucin secretion in weaning piglets. Methods: fifty-two piglets weaned at 21 days of age were fed a basal diet supplemented with four LPS levels (0.0, 0.3, 0.5, and 1.0 µg/mg) during 10 days. Piglets were slaughtered on days 1, 5, 7, and 10 post-weaning and samples of small and large intestine were taken for histochemical staining to determine goblet cell population and type of mucins produced (acidic, sulphated, non-sulphated, or neutral). Results: acidic mucin was reduced on day 5 post-weaning independently of the dietary LPS level supplied to piglets. Recovery of acidic mucins was observed during days 7 and 10 post-weaning. Neutral mucins increased on day 5 and decreased on days 7 and 10 post-weaning. High LPS levels decreased goblet cells population and secretion of all types of mucins. This effect was remarkably high for diet two (D2: 0.5 mg LPS/mg food). Conclusions: early weaning (21 d) and LPS addition to the diet affect mucin secretion and intestinal epithelium integrity by modifying goblet cell populations and their balance between acidic and neutral mucin secretion. These findings explain some abnormalities related with post-weaning diarrhea syndrome and help to explain its pathophysiology.

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Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood

Cited by 61 publications

References 34 publications

Down-regulation of Stathmin Is Required for the Phenotypic Changes and Classical Activation of Macrophages

Down-regulation of Stathmin Is Required for the Phenotypic Changes and Classical Activation of Macrophages

Neutrophil Cellular Responses to Various Salmonella typhimurium LPS Chemotypes

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

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