Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats

Gavrilović, Ljubica; Spasojević, Nataša; Živković, Maja; Dronjak, Sladjana

doi:10.1590/s0100-879x2009005000040

Cited by 7 publications

(5 citation statements)

References 26 publications

(22 reference statements)

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“…Real-time PCR was performed using SYBR green PCR master mix (Roche). We decided to use cyclophilin A as the only reference gene, because it has been demonstrated that the expression of cyclophilin A does not change in the brain or other tissues using protocols similar to ours or using other type of stimuli (Gavrilovic et al 2009;Langnaese et al 2008;Nankova et al 1998;Thal et al 2008). The following specific primers were used at 0.5 lM CRF (forward: 5 0 -ccagggcagagcagttagct-3 0 ; Neurotox Res (2011) 20:289-300 291 reverse: 5 0 -gcaacatttcatttcccgataatc-3 0 ), Nur77 (forward: 5 0 -ggcatggtgaaggaagttgt-3 0 ; reverse: 5 0 -gattggtaggggaggcat ct-3 0 ), and cyclophilin A (forward: 5 0 -cgtgctctgagcactggg gagaaa-3 0 reverse: 5 0 -catgccttctttcaccttcccaaagac-3 0 ).…”

Section: Rna Extraction and Semi-quantitative Real-time Rt-pcrmentioning

confidence: 99%

Repeated Immobilization Stress Increases Nur77 Expression in the Bed Nucleus of the Stria Terminalis

et al. 2011

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The transcription factor Nur77 has been identified as a neuronal activation marker of stressful stimuli in the central nervous system. Nur77 plays a key role at all levels of the hypothalamus-pituitary-adrenal axis during the stress response. However, the participation of Nur77 in extra-hypothalamic responses to stress is unknown. In this study, we studied the impact of acute and repeated immobilization stress on Nur77 expression in the bed nucleus of stria terminalis (BNST), a region involved in autonomic, neuroendocrine, and behavioral responses to stress. After a single session of immobilization stress we observed a significant increase of Nur77-like immunoreactivity in the BNST. This effect is not lost with repeated exposure to stress, since Nur77-like immunoreactivity and Nur77 mRNA in BNST were increased after the fifteenth stress session. The administration of desipramine, a specific inhibitor of noradrenaline reuptake, prevented the increase in Nur77-like immunoreactivity and mRNA induced by stress in rats subjected to repeated exposure to immobilization stress. Our results show that acute and repeated stress modulates Nur77 expression in BNST and suggest that Nur77 participates in extra-hypothalamic responses to stress.

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Section: Rna Extraction and Semi-quantitative Real-time Rt-pcrmentioning

confidence: 99%

Repeated Immobilization Stress Increases Nur77 Expression in the Bed Nucleus of the Stria Terminalis

et al. 2011

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“…Previous in vivo studies have focused on the localization and regulation of PNMT in the hearts of rats and mice under basal and stress conditions (Gavrilovic et al, 2009;Gavrilovic et al, 2010;Krizanova et al, 2007;Krizanová et al, 2001;Kvetnansky et al, 2004). Kvetnansky and coauthors (2004) have shown in Sprague-Dawley rats that the LA has the highest basal expression of PNMT mRNA and protein, followed by the RA, with the ventricles expressing much lower basal amounts of PNMT mRNA and protein.…”

Section: Discussionmentioning

confidence: 99%

Cardiac phenylethanolamine N-methyltransferase: localization and regulation of gene expression in the spontaneously hypertensive rat

Peltsch

Khurana

Byrne

et al. 2016

Can. J. Physiol. Pharmacol.

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https://mc06.manuscriptcentral.com/cjpp-pubs ABSTRACTPhenylethanolamine N-methyltransferase (PNMT) is the terminal enzyme in the catecholamine biosynthetic pathway responsible for adrenaline biosynthesis. Adrenaline is involved in the sympathetic control of blood pressure; it augments cardiac function by increasing stroke volume and cardiac output. Genetic mapping studies have linked the PNMT gene to hypertension. This study examined the expression of cardiac PNMT and changes in its transcriptional regulators in the spontaneously hypertensive (SHR) and wild type Wistar-Kyoto (WKY) rats. SHR exhibit elevated levels of corticosterone, and lower levels of the cytokine IL-1 β, revealing systemic differences between SHR and WKY. PNMT mRNA was significantly increased in all chambers of the heart in the SHR, with the greatest increase in the right atrium. Transcriptional regulators of the PNMT promoter show elevated expression of Egr-1, Sp1, AP-2 and GR mRNA in all chambers of the SHR heart, while protein levels of Sp1, Egr-1 and GR were elevated only in the RA. Interestingly, only AP-2 protein-DNA binding was increased, suggesting it may be a key regulator of cardiac PNMT in SHR. This study provides the first insights into the molecular mechanisms involved in the dysregulation of cardiac PNMT in a genetic model of hypertension.

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“…The effective biological processes of the LRP11 gene include response to heat, cold, hunger, mechanical stimuli and stress. In general, the LRP11 gene is an integral part of the cell membrane (22)(23). The molecular functions of the FUBP1 gene include binding to nucleic acid, DNA, RNA, and pro- Figures 4, 5, and 6 show the different QMEAN z-scores, including QMEAN, Cβ interactions, all-atom interactions, solvation, and torsion for the LRP11, FUBP1, and TET1 genes.…”

Section: Ontology Of Lrp11 Fubp1 and Tet1 Genesmentioning

confidence: 99%

The expression and clinical value of LRP11, FUBP1 and TET1 in cervical cancer

Xiaojie Zhao,

Suhua Li,

Jiexin Wen

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats

Cited by 7 publications

References 26 publications

Repeated Immobilization Stress Increases Nur77 Expression in the Bed Nucleus of the Stria Terminalis

Repeated Immobilization Stress Increases Nur77 Expression in the Bed Nucleus of the Stria Terminalis

Cardiac phenylethanolamine N-methyltransferase: localization and regulation of gene expression in the spontaneously hypertensive rat

The expression and clinical value of LRP11, FUBP1 and TET1 in cervical cancer

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