Apolipoprotein E polymorphism in Brazilian dyslipidemic individuals: Ouro Preto study

Abstract

The influence of apolipoprotein E alleles and genotypes on plasma lipid levels was determined in 185 individuals of mixed ethnicity living in Ouro Preto, Brazil. DNA was obtained from blood samples and the genotypes were determined by an RFLP-PCR procedure. The *3 allele was the most frequent (72%), followed by *4 (20%) and *2 (8%); *4 frequency was higher and *2 frequency was lower in the dyslipidemic group than in the normal control group. The *2 carriers presented lower LDL and total cholesterol levels compared to the *3 and *4 carriers. All six expected genotypes were observed in the individuals genotyped: E2/2 (2.1%), E4/4 (2.7%), E2/4 (3.7%), E2/3 (8.0%), E3/3 (53.3%), E3/4 (29.9%); no difference in genotype frequencies was found between the normal and dyslipidemic groups. Compared with *2, the presence of *3 increases more than two times the risk for dyslipidemia (OR = 2.31; P = 0.025; 95% CI = 1.06-5.06) and the presence of *4 increases it three times (OR = 3.31; P = 0.006; 95% CI = 1.36-8.04). The only significant effect of genotype was an increased risk for dyslipidemia in the *4 genotype carriers (E3/4 + E4/ 4) compared with the *2 genotype carriers (E2/2 + E2/3) with OR = 3.69 (95% CI = 1.25-10.88). The present study indicates that in the Ouro Preto admixed population the presence of APOE *2 can confer a protective effect, whereas the presence of APOE *4 implies an enhanced risk for dyslipidemia.

Apolipoprotein E Gene Polymorphism And Its Effect On Plasma Lipids In Arteriosclerosis

“…Mendes-Lana A demonstrated that the presence of Apo E2 can confer a protective effect, the presence of Apo E4 implies an enhanced risk of dyslipidemia [26], and our results are in accordance with above studies for E 4 allele. The study confirms the significant association between ApoE4 allele and hyperlipidemia.…”

Gravidade da lesão angiográfica coronariana e polimorfismo da APOE nas síndromes coronarianas agudas

“…On the other hand, the frequency of the allele was lower than the one observed in northern European countries, such as Ireland (22%) 28 and lower than the one found in the town of Ouro Preto, state of Minas Gerais, Brazil (20%) 29 , where the African contribution for the formation of the population was higher.…”

Cardiovascular risk factors in children and adolescents living in an urban area of Southeast of Brazil: Ouro Preto Study

“…The occurrence of dyslipidemia was higher among adults bearing allele 4 [29]. Additionally, another study in this population showed that lipoprotein (a) serum levels were highly correlated with the occurrence of ischaemic heart disease and the Framingham risk score [6].…”