Adhesion molecule profiles of B-cell non-Hodgkin's lymphomas in the leukemic phase

Abstract

We evaluated the expression of 10 adhesion molecules on peripheral blood tumor cells of 17 patients with chronic lymphocytic leukemia, 17 with mantle-cell lymphoma, and 13 with nodal or splenic marginal B-cell lymphoma, all in the leukemic phase and before the beginning of any therapy. The diagnosis of B-cell non-Hodgkin's lymphomas was based on cytological, histological, immunophenotypic, and molecular biology methods. The mean fluorescence intensity of the adhesion molecules in tumor cells was measured by flow cytometry of CD19-positive cells and differed amongst the types of lymphomas. Comparison of chronic lymphocytic leukemia and mantle-cell lymphoma showed that the former presented a higher expression of CD11c and CD49c, and a lower expression of CD11b and CD49d adhesion molecules. Comparison of chronic lymphocytic leukemia and marginal B-cell lymphoma showed that the former presented a higher expression of CD49c and a lower expression of CD11a, CD11b, CD18, CD49d, CD29, and CD54. Finally, comparison of mantle-cell lymphoma and marginal B-cell lymphoma showed that marginal B-cell lymphoma had a higher expression of CD11a, CD11c, CD18, CD29, and CD54. Thus, the CD49c/CD49d pair consistently demonstrated a distinct pattern of expression in chronic lymphocytic leukemia compared with mantle-cell lymphoma and marginal B-cell lymphoma, which could be helpful for the differential diagnosis. Moreover, the distinct profiles of adhesion molecules in these diseases may be responsible for their different capacities to invade the blood stream.

α7 Helix Region of αI Domain Is Crucial for Integrin Binding to Endoplasmic Reticulum Chaperone gp96

“…2C). Of particular interest, gp96 plays a key role in the folding and cell surface expression of multiple integrin subunits, including ␣1, ␣2, ␣4, ␣D, ␣L, ␣M, ␣X, ␣V, ␣E, ␤2, ␤5, ␤6, ␤7, and ␤8 (32,34,35,42), many of which are critically required for tumor growth and metastasis (2)(3)(4)(5)(6)(7)(8)(9). In this study, we found that competitive blocking of the gp96-integrin interaction by TAT-␣7 helix peptide decreased surface expression and maturation of not only integrin ␣L but also of other integrins (i.e.…”

“…In addition, increased expression of the integrin ␣6␤4 stimulates the survival of breast cancer cells (4,5), and elevated expression of integrin ␣5␤1 correlates with decreased survival in patients with lymph node-negative non-small-cell lung carcinoma (6). Moreover, integrin ␣L is up-regulated in CD44 stimulationinduced adhesion of colon cancer cells (7), and integrin ␣L, ␣X, ␤1, ␤2, and ICAM are highly expressed in marginal zone B-cell lymphoma (8,9). Furthermore, integrins on cancer stem cells have also been reported to play essential roles for cancer initiation and progression (10).…”

The Role of CD11c Expression in the Diagnosis of Mantle Cell Lymphoma

“…11,14,20,22,[24][25][26][27][28][29] The presence of cyclin D1 immunoreactivity and/or detection of t(11;14) by karyotyping, FISH, PCR, or Southern blot was confirmed in 43 of these MCL cases. However, results of such confirmatory studies were not reported for the 7 CD11c+ cases.…”

Diffuse large B-cell lymphoma presenting in the leukemic phase

“…The available data on DLBC do not support the hypothesis that a different profile of adhesion molecules plays a role in the pathogenesis of bone marrow involvement. 5 , 6 …”