&lt;a name="home"&gt;&lt;/a&gt;Activation of neural cholecystokinin-1 receptors induces relaxation of the isolated rat duodenum which is reduced by nitric oxide synthase inhibitors

Martins, S R; Oliveira, Ricardo Brandt de; Ballejo, Gustavo

doi:10.1590/s0100-879x2006000200014

Cited by 8 publications

(5 citation statements)

References 15 publications

(22 reference statements)

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“…The result of the present study are consistent with the research of Martins et al, which indicated that endogenous CCK inhibited the spontaneous contraction of duodenal longitudinal muscle via V 1 receptors on myenteric neurons [5]. The CCK 1 receptors were located on vagal afferent neurons [22], myenteric neurons, and interstitial cell of Cajal [11].…”

Section: Discussionsupporting

confidence: 93%

CCK mediated the inhibitory effect of oxytocin on the contraction of longitudinal muscle strips of duodenum in male rats

Feng

Che

et al. 2010

Pflugers Arch - Eur J Physiol

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The aim of the present study was to investigate the effect of oxytocin (OT) on duodenum motility in rats and the possibility that cholecystokinin (CCK) was involved in this process. The isometric contraction of longitudinal muscle strips of duodenum was monitored by polygraph. ELISA was used to measure the concentration of CCK and OT in duodenum. CCK mRNA was assayed by RT-PCR. Oxytocin receptor (OTR) and CCK in duodenum were located by immunohistochemistry and immunofluorescence staining. OT (10⁻⁵ and 10⁻⁶ M) inhibited the spontaneous contraction of the muscle strips. On the contrary, atosiban (OT receptor antagonist), lorglumide (CCK₁ receptor antagonist), and tetrodotoxin (TTX, blocker of voltage-dependent Na(+) channel on nerve fiber) excited the contraction. The inhibitory effect of OT on duodenal motility was reversed by pretreatment of atosiban, lorglumide, or TTX. Exogenous OT did not influence the expression of OT mRNA in duodenum but increased the concentration of CCK in the culture medium of the cells isolated from longitudinal muscle myenteric plexus. The OTR and CCK were co-expressed in the neurons of the myenteric plexus in duodenum. We concluded that OT inhibited the contraction of the LD spontaneous contraction of rats in vitro. This effect was mediated by the CCK released from the neurons of the myenteric plexus in duodenum.

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Section: Discussionsupporting

confidence: 93%

CCK mediated the inhibitory effect of oxytocin on the contraction of longitudinal muscle strips of duodenum in male rats

Feng

Che

et al. 2010

Pflugers Arch - Eur J Physiol

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“…There is rather no doubt that CCK stimulates phasic contractions in the small intestine (Bueno and Praddaude 1979;Giuliani et al 1990;Mukhopadhyay et al 1977;Romaƒski 2002) although there are some reports showing that duodenum did not respond significantly to CCK administration especially when the studies were performed in fasted animals (Wingate et al 1978). Its action can be also inhibitory as studied on isolated rat duodenum (Martins et al 2006). The effects of CCK on tonic contractions within the gastrointestinal tract have not been extensively examined and the effect of CCK on the tonic component in the small bowel in sheep is unknown.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cholecystokinin-Octapeptide and Cerulein on Phasic and Tonic Components in Ovine Duodenum with Special Reference to the 'Minute Rhythm'

Romanski¹

2007

Acta Vet. Brno

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Cholecystokinin (CCK) can affect phasic contractions and the minute rhythm (MR) in ovine duodenum but its effect on the tonic component remains unclear. Thus, the aim of this study was to assess whether the hormone exerts significant changes on phasic and tonic components of the duodenal motor activity and on phasic and tonic components of the duodenal MR. Mechanical and electrical activities of the duodenum were recorded in four sheep before and after slow intravenous cholecystokinin octapeptide (CCK-OP, doses 20, 200 and 2000 ng/kg b.w.) and cerulein (doses 1, 10 and 100 ng/kg b.w.) administration in the course of phase 2b of the migrating motor complex. During 5-20-minute periods the area under contraction curve for phasic, tonic and total motor activity was measured for the whole curve and separately for the MR-related activity. It was found that both CCK peptides stimulate phasic and tonic components of the duodenal motor activity as well as both these components of the duodenal MR. The effect of CCK peptides on the tonic component was stronger than on the phasic component. These effects were similar in non-fasted and fasted animals. CCK-OP evoked slightly greater effect than cerulein. The effects of these CCK peptides on phasic and tonic components of the MR were similar. It is concluded that CCK-OP and cerulein stimulate both phasic and tonic components of the duodenal motor activity and phasic and tonic components related to the MR in sheep. , small bowel, myoelectrical and motor activity, cholecystokinin Ruminants

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“…Similar effects were reported for cerulein (Bertaccini et al, 1968;Ormas et al, 1984;Niederau and Karaus, 1991;Romański, 2004). Other reports indicate that the The explanations for Table 1 values expresseding/s/min; 0-10 -one-minute consecutive periods; 0 -control period, 1-10 -period safter hormone administration; dose of the hormone expressed in ng/kg; duration of hormone administration expressed in seconds (s); the values of three previous control periods, insignificantly different from period 0, are not shown; statistical significances: a P < 0.05; b P < 0.01; c P < 0.001 vs. relevant value of period 0; other explanations see the text The explanation see Table 1 effect of CCK action on duodenal motility can be inhibitory or no effect can be observed while some authors observed a simultaneous stimulatory effect in the jejunum (Fleckenstein and Öigaard, 1977;Bueno and Praddaude, 1979;Giralt and Vergara, 2000;Martins et al, 2006). This confirms the view that the character of CCK action can be different in the various small intestinal segments.…”

Section: Discussionmentioning

confidence: 94%

“…Pretreatment with CCK receptor antagonist reverses this action (Niederau and Karaus, 1991;Xu et al, 1998). However, the opposite and dual effects of CCK-OP and its amphibian analogue, cerulein, have been reported (Giuliani et al, 1990;Martins et al, 2006). In sheep, CCK peptides also inhibit the arrival of the MMC in the upper small intestine, but their effect on intestinal motility has not been fully elucidated so far in spite of several studies performed (Bueno and Praddaude, 1979;Cottrell and Iggo, 1984;Ormas et al, 1984;Onaga et al, 1997;Romański, 2004Romański, , 2007b.…”

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confidence: 99%

Effects of cholecystokinin-octapeptide and cerulein on small-intestinal motility in sheep

Romanski¹

2010

CZECH J ANIM SCI

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Cholecystokinin (CCK) affects the intestinal motility but in ruminants the question has not been entirely explored. The aim of this study was to examine the precise effects of CCK-octapeptide (CCK-OP) and its amphibian analogue, cerulein, on duodenal motor activity in unfasted rams in the course of chronic experiments. Five rams underwent the implantation of a strain gauge force transducer to the duodenal wall, and -additionally -the bipolar platinum electrodes to the duodenal bulb, distal duodenum, near the strain gauge force transducer, and proximal jejunum. During continuous motor recordings, 0.15M NaCl or CCK peptides were administrated intravenously. Injections of CCK-OP at doses of 20 (over 30 s), 200 (over 30 or 60 s), and 2 000 (over 30, 60, or 120 s) ng/kg of body weight and injections of cerulein at doses of 1, 10, or 100 ng/kg (given over the same periods) were each administered in the course of duodenal phase 1, 2a, or 2b of the migrating motor complex (MMC), i.e. 5 min after the onset of each phase. Injections of the smallest doses of CCK peptides exerted a slight and mostly insignificant effect on the duodenal areas under contraction (AUC). In the duodenum, the moderate doses of the hormones evoked short stimulatory effects followed by longer inhibitory biphasic effects on AUC. These effects were inversely related to the duration of the hormone injection. It is concluded that CCK evokes stimulatory and inhibitory (biphasic) physiological effects on duodenal motility in sheep.

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<a name="home"></a>Activation of neural cholecystokinin-1 receptors induces relaxation of the isolated rat duodenum which is reduced by nitric oxide synthase inhibitors

Cited by 8 publications

References 15 publications

CCK mediated the inhibitory effect of oxytocin on the contraction of longitudinal muscle strips of duodenum in male rats

CCK mediated the inhibitory effect of oxytocin on the contraction of longitudinal muscle strips of duodenum in male rats

Effect of Cholecystokinin-Octapeptide and Cerulein on Phasic and Tonic Components in Ovine Duodenum with Special Reference to the 'Minute Rhythm'

Effects of cholecystokinin-octapeptide and cerulein on small-intestinal motility in sheep

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