Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis

Abstract

The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as anti-Leishmania total antigen antibodies. When cross-reactivity was tested, chronic Chagas' disease patients (N = 10) did not show any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.

Enzyme-linked Immunosorbent Assay for Superoxide Dismutase–Excreted Antigen in Diagnosis of Sylvatic and Andean Cutaneous Leishmaniasis of Peru

“…The absence of crossreactivity with Chagas' disease sera is important, because the endemic areas of the two diseases often overlap. 16 In summary, we developed an ELISA that can detect antibodies in human patients with SL and ACL. Leishmania SODe was shown to be a good antigen, showing an overall sensitivity and high specificity for routine use for serodiagnosis of diseases.…”

“…8 At the moment, serology is helpful for visceral forms because antibodies in cutaneous disease tend to be undetectable or present in low titer. For improvement of the specificity of the serodiagnostic tests, a search for defined Leishmania -specific antigens has been undertaken, and various candidates have been proposed; the k39 antigen from Leishmania chagasi and its use in the serodiagnosis of visceral leishmaniasis 9,10 : a membrane antigen of 32 kd (P32) present in L. donovani infantum with a high sensitivity and specificity of 94% in the serodiagnosis of Mediterranean visceral leishmaniasis, 11 two hydrophilic antigens (k9 and k26) of L. chagasi , 12 a secreted acid phosphatase, 13 an A2 antigen, 14 an excreted antigen from the culturing of the parasite in medium free of proteins and serum, 15 two thermal-shock proteins (Hsp 70 and 83), 16 a recombinant proteinase cistein, 17 three recombinant antigens (rH2A, KMP11, and Protein Q 18 ) rk26 and rK39 antigens from Leishmania infantum used in ELISAs provided very high sensitivities for the detection of symptomatic dogs, meanwhile, the rA2 protein from Leishmania donovani is more sensitive for asymptomatic dogs , 19 more recently, an antigen excreted by amastigote forms of L. amazonensis 20 and, an excreted superoxide dismutase by L. infantum useful in diagnosing canine visceral leishmaniasis. 21 The aim of this study was to identify a new antigen excreted (FeSOD) by L.…”

Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis

“…Some of these antigens, L. major Hsp60 [71] and L. braziliensis Hsp70 [72], were cloned and the products tested using cutaneous leishmaniasis and mucocutaneous samples from Colombia with promising results. L. (L.) infantum Hsp83 [73] was also tested using a limited number of cutaneous and mucocutaneous samples and showed 100% reactivity, interestingly without any cross-reactivity with Chagas' disease samples. Considering those data showing a certain species specificity of the antibody reactivity, the development of assays using combined recombinant antigens should be contemplated.…”

Epitope Mapping of the HSP83.1 Protein of Leishmania braziliensis Discloses Novel Targets for Immunodiagnosis of Tegumentary and Visceral Clinical Forms of Leishmaniasis

“…Previous studies have described that heat shock proteins, such as HSP83 and other chaperones, are among the most abundant proteins detected in Leishmania antigenic extracts and have obtained promising results that can be applied to the development of diagnostic kits (38)(39)(40)(41)(42)(43)(44). Here, the antigenicity of recombinant HSP83.1 (rHSP83.1) and three epitopes predicted in this protein were tested with sera from the TL, VL, and CVL groups.…”