IS1245 restriction fragment length polymorphism typing of Mycobacterium avium from patients admitted to a reference hospital in Campinas, Brazil

Abstract

Mycobacterium avium is an important pathogen among immunodeficient patients, especially patients with AIDS. The natural history of this disease is unclear. Several environmental sources have been implicated as the origin of this infection. Polyclonal infection with this species is observed, challenging the understanding of its pathogenesis and treatment. In the present study 45 M. avium strains were recovered from 39 patients admitted to a reference hospital between 1996 and 1998. Species identification was performed using a species-specific nucleic acid hybridization test (AccuProbe ® ) from Gen-Probe ® . Strains were genotyped using IS1245 restriction fragment length polymorphism typing. Blood was the main source of the organism. In one patient with disseminated disease, M. avium could be recovered more than once from potentially sterile sites. Strains isolated from this patient had different genotypes, indicating that the infection was polyclonal. Four patient clones were characterized in this population, the largest clone being detected in eight patients. This finding points to a common-source transmission of the organism.

Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains

“…In summary, this study shows that the rapid, easy, and inexpensive PCR typing based on two M. avium-insertion sequences is useful for epidemiological studies such as detection of polyclonal infection, an event that may have treatment implications (Arbeit et al 1993, Saad et al 2000, Panunto et al 2003). However we suggest to strict its use in small sampling from select set because of the limitation on analysis of patterns with difference in one band.…”

Molecular features ofMycobacterium aviumhuman isolates carrying a single copy of IS1245and IS1311per genome

“…1): patient 3 (strains 37B and 38B) and patient 4 (strains 43B and 44B). Detection of MA polyclonal infections has been variously reported in the literature (Slutsky et al , 1994; von Reyn et al , 1995; Matsiota‐Bernard et al , 2000; Oliveira et al , 2000; Saad et al , 2000; Dvorska et al , 2002; Panunto et al , 2003).…”

Methods for Detecting Mycobacterial Mixed Strain Infections–A Systematic Review

“…Our review showed that MSIs involving NTM have not been investigated to the same extent as compared to those caused by M. tuberculosis ( Table 2). Amongst the 121 mycobacterial studies identified, 26 (21.5%) examined NTM, of which 17 (14.0%) and eight (6.6%) found MSIs in humans and animals, respectively, whereas one report identified MSIs in both human (Arbeit et al, 1993;Slutsky et al, 1994;Von Reyn et al, 1995;Devallois and Rastogi, 1997;Picardeau et al, 1997;Wallace et al, 1998;Legrand et al, 2000a,b;Oliveira et al, 2000;Saad et al, 2000;Dvorska et al, 2002;Panunto et al, 2003;Ohkusu et al, 2004;De Sequeira et al, 2005;Fujita et al, 2014;García-Pedrazuela et al, 2015;Kimizuka et al, 2019) and animal (Dvorska et al, 2007;Shitaye et al, 2008;Furphy et al, 2012;Gerritsmann et al, 2014;Johansen et al, 2014;Gioffré et al, 2015;Podder et al, 2015;Davidson et al, 2016;Pfeiffer et al, 2017) populations simultaneously (Pate et al, 2008). Many early studies used pulsed field gel electrophoresis (PFGE) to identify MSIs in 14.3-100% of patients infected with MAC bacteria (Arbeit et al, 1993;Slutsky et al, 1994;Von Reyn et al, 1995;Wallace et al, 1998;Ohkusu et al, 2004).…”

“…In a separate study, Oliveira et al (2000) used RFLP analysis of hsp65 PCR products, IS1245 and IS1311, respectively, to detect an M. avium MSI. Though the presence of IS1245-RFLP may be supplemented using additional methods, studies have shown it alone is capable of identifying 2.6-100% of NTM MSIs in samples from human subjects, some with HIV-AIDS (Supplementary Table 2) (Picardeau et al, 1997;Saad et al, 2000;Dvorska et al, 2002;Panunto et al, 2003;Pate et al, 2008). In addition, a study examined 41 samples from 14 AIDS patients using MaDRE-PCR, which found four cases with multiple banding patterns, though re-evaluation using IS1245-RFLP only confirmed two as M. avium MSIs (De Sequeira et al, 2005).…”