A method for multiple sequential analyses of macrophage functions using a small single cell sample

Abstract

Microbial pathogens such as bacillus Calmette-Guérin (BCG) induce the activation of macrophages. Activated macrophages can be characterized by the increased production of reactive oxygen and nitrogen metabolites, generated via NADPH oxidase and inducible nitric oxide synthase, respectively, and by the increased expression of major histocompatibility complex class II molecules (MHC II).

Interferon Regulatory Factor (IRF)-1 Is a Master Regulator of the Cross Talk between Macrophages and L929 Fibrosarcoma Cells for Nitric Oxide Dependent Tumoricidal Activity

“…Importantly, resident macrophages as well as L929 cells cultured alone did not produce detectable levels of NO ( S1b Fig . ), indicating that resident macrophages are not activated, confirming previous work from our laboratory, where we compared resident and BCG activated macrophages [ 28 ].…”

“…§ TNFα rich mouse serum was obtained by infecting mice with 2.5x10 7 Mycobacterium bovis bacilli, followed by treatment with 35 μg LPS 15 days post injection. Mice were euthanized 90 min later and blood harvested for serum preparation [ 28 ].…”

[Fe(CN)5(isoniazid)]3−: An iron isoniazid complex with redox behavior implicated in tuberculosis therapy

“…Hydrogen peroxide levels of at least 20 M have been reported [54], which could ensure enough for triggering IQG607.…”

Increase of cellular recruitment, phagocytosis ability and nitric oxide production induced by hydroalcoholic extract from Chenopodium ambrosioides leaves

“…H 2 O 2 -release and NO-production was determined in a single macrophage sample using a method previously described by Nascimento et al (2003). To evaluate H 2 O 2 -release, a horseradish peroxidase-dependent phenol red oxidation micro assay was used (Pick and Mizel, 1981).…”