Distribution of versican and hyaluronan in the mouse uterus during decidualization

Martín, Sebastián San; Soto-Suazo, Maurício; Zorn, Telma Maria Tenório

doi:10.1590/s0100-879x2003000800013

Cited by 29 publications

(30 citation statements)

References 19 publications

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“…However, and opposite to all expectations, infusion with 4MU resulted in strong embryo attachment in all treated ewes (4/4). These results are in line with previous reports that showed disappearance of HA at the maternal-embryo interface at the time of implantation in mice on Day 5-7 of pregnancy Papaioannou 1992, 1993;San Martin et al 2003). Moreover, increased decidual hyaluronan levels due to altered HA catabolism (by hyaluronidases) at the feto-maternal interface resulted in spontaneous abortion in mice on gestation Day 7.5 (Cordo-Russo et al 2009).…”

Section: Discussionsupporting

confidence: 92%

Influence of hyaluronan on endometrial receptivity and embryo attachment in sheep

Marei

Wathes

Raheem

et al. 2017

Reprod. Fertil. Dev.

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(200 words for RFD)An increasing number of reports suggests a role of hyaluronan (HA) in female reproduction and interest in its application in assisted reproduction is rising. However, there are contrasting data about the effectiveness of adding HA to the embryo-transfer medium on improving pregnancy rates. Using sheep as an experimental model, the studies reported here analysed the impact of HA infusion into the uterus on embryo attachment to uterine luminal epithelium (LE) and expression of selected markers of uterine receptivity. On Day 14 after natural mating (preattachment), uterine horns were infused with either (n = 4 each): PBS (control), HA (1 mg mL-1), HA + hyaluronidase 2 (Hyal2; 300 IU mL-1) or 4-methyl-umbelliferone (HA-synthesis inhibitor; 4MU, 1 mM). HA immunostaining on uterine sections collected on Day 17 was negative in the 4MU group and weak in the HA+Hyal2 group. In contrast to 4MU, which resulted in 100% attachment, HA infusion blocked embryo attachment in all treated animals.This was accompanied by the disappearance of mucin 1 and increased expression of osteopontin and CD44v6 in the LE of uteri with attached embryos. In conclusion, the presence of HA at the embryo-maternal interface during embryo implantation resulted in reduced endometrial receptivity and inhibited the interaction of trophoblasts with the LE, whereas clearance of HA favoured embryo attachment.3

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Section: Discussionsupporting

confidence: 92%

Influence of hyaluronan on endometrial receptivity and embryo attachment in sheep

Marei

Wathes

Raheem

et al. 2017

Reprod. Fertil. Dev.

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“…One possibility is that the specialized extracellular matrix produced by decidual stromal cells provides an insufficient scaffold for interstitial DC migration. Along with many unusual features (35,36), this matrix contains low amounts of hyaluronan, a molecule known to be required for DC migration likely through interactions with its cell-surface receptor CD44 (37)(38)(39)(40)(41). Second, it is possible that the periphery of the decidua creates some sort of physical barrier, although the relatively homogenous distribution of decidual DCs within LPS-treated mice ( Figure 8D) would argue against this idea.…”

Section: Figurementioning

confidence: 99%

Dendritic cell entrapment within the pregnant uterus inhibits immune surveillance of the maternal/fetal interface in mice

Collins¹,

Tay²,

Erlebacher³

2009

J. Clin. Invest.

156

205

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Embryo implantation induces formation of the decidua, a stromal cell-derived structure that encases the fetus and placenta. Using the mouse as a model organism, we have found that this tissue reaction prevents DCs stationed at the maternal/fetal interface from migrating to the lymphatic vessels of the uterus and thus reaching the draining lymph nodes. Strikingly, decidual DCs remained immobile even after being stimulated with LPS and exhibiting responsiveness to CCL21, the chemokine that drives DC entry into lymphatic vessels. An analysis of maternal T cell reactivity toward a surrogate fetal/placental antigen furthermore revealed that regional T cell responses toward the fetus and placenta were driven by passive antigen transport and thus the tolerogenic mode of antigen presentation that predominates when there is negligible input from tissue-resident DCs. Indeed, the lack of involvement of tissue-resident DCs in the T cell response to the fetal allograft starkly contrasts with their prominent role in organ transplant rejection. Our results suggest that DC entrapment within the decidua minimizes immunogenic T cell exposure to fetal/placental antigens and raise the possibility that impaired development or function of the human decidua, which unlike that of the mouse contains lymphatic vessels, might lead to pathological T cell activation during pregnancy.

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“…Hyaluronic acid and versican have also been detected in the endometrial stroma during the preimplantation period. After implantation, hyaluronic acid disappears from the decidual region in the immediate vicinity of the implantation chamber, whereas versican accumulates in this region, suggesting that these proteoglycans play an important role in the proliferation and differentiation of endometrial fibroblasts into decidual cells, influencing trophoblast invasion (San Martin et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Histochemical Study of Early Embryo Implantation in Rats

Spósito

Santos

2011

Int. J. Morphol.

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SUMMARY:Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eosin, toluidine blue at pH 4.0 (TB), and Xylidine Ponceau at pH 2.5 (XP). In non-pregnant females, the uterine layers could be clearly distinguished and showed the normal histology of the organ. In pregnant females, an increase in the number of cells and a reduction in the interstitial space were observed in the endometrium close to the implantation sites. The blastocyst was partially inserted in the endometrium, with the observation of the inner cells mass around the blastocyst cavity surrounded by trophoblastic cells. TB staining revealed mild metachromatic basophilia, which was more evident in the endometrial stroma around the implantation site. Histochemical staining with XP was also more intense in the stroma close to the site of implantation. On the other hand, histochemical staining with either TB or XP was more discrete at sites distant from the conceptus. This study demonstrated changes in the endometrial stroma in areas adjacent to the site of embryo implantation, with variations in glycosaminoglycans and proteins as demonstrated by the detection of anionic and cationic radicals, respectively.

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Distribution of versican and hyaluronan in the mouse uterus during decidualization

Cited by 29 publications

References 19 publications

Influence of hyaluronan on endometrial receptivity and embryo attachment in sheep

Influence of hyaluronan on endometrial receptivity and embryo attachment in sheep

Dendritic cell entrapment within the pregnant uterus inhibits immune surveillance of the maternal/fetal interface in mice

Histochemical Study of Early Embryo Implantation in Rats

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