Effect of perimuscular injection of Bothrops jararacussu venom on plasma creatine kinase levels in mice: influence of dose and volume

Calil-Elias, Sabrina; Thattassery, Emil; Martínez, Ana Maria Blanco; Melo, Paulo A.

doi:10.1590/s0100-879x2002001000018

Cited by 20 publications

(14 citation statements)

References 8 publications

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“…Resultados laboratoriais Na avaliação do eritrograma verificou-se acentuada anemia normocítica normocrômica, ocasionada pelas hemorragias após a inoculação, alteração também descrita em bovinos por Oliveira et al (2004b) e Caldas et al (2008 e em ovinos por Aragão et al (2010) (Melo & Suares-Kurtz 1987, Santos et al 1992, Calil-Elias et al 2002, Sifuentes et al 2008) e em bovinos inoculados experimentalmente . França & Málaque (2009) verificaram que em humanos, os níveis de CK e DHL encontravam-se aumentados em pacientes com processo inflamatório no local da picada.…”

Section: Quadro Clínico Geralunclassified

Aspectos clínico-patológicos do envenenamento botrópico experimental em equinos

et al. 2011

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Estudou-se as alterações clínico-patológicas e laboratoriais em equinos, inoculados experimentalmente com a peçonha de Bothropoides jararaca, Bothrops jararacussu, Bothrops moojeni e Bothropoides neuwiedi, com a finalidade de fornecer subsídios para o diagnóstico do envenenamento pela picada dessas. Os venenos liofilizados foram diluídos em 1ml de solução fisiológica e administrados a seis equinos, por via subcutânea, nas doses de 0,5 e 1mg/kg (B. jararaca), 0,8 e 1,6mg/kg (B. jararacussu), 0,205mg/kg (B. moojeni) e 1mg/kg (B. neuwiedi). Todos os equinos, menos os que receberam o veneno de B. jararacussu, morreram Os sinais clínicos iniciaram-se entre 8min e 2h10min após a inoculação. O período de evolução variou, nos quatro casos de êxito letal, de 24h41min a 70h41min, e nos dois equinos que se recuperaram foi de 16 dias. O quadro clínico, independente do tipo de veneno e das doses, caracterizou-se por aumento de volume no local da inoculação, arrastar da pinça do membro inoculado no solo, inquietação, apatia, diminuição da resposta aos estímulos externos, mucosas pálidas e hemorragias. Os exames laboratoriais revelaram anemia normocítica normocrômica com progressiva diminuição no número de hemácias, da hemoglobina e do hematócrito, e leucocitose por neutrofilia. Houve aumento de alamina aminotransferase, creatinaquinase, dehidrogenase láctica, ureia e glicose, bem como aumento do tempo de ativação da protrombina e do tempo de tromboplastina parcial ativada. Os achados de necropsia foram extensas hemorragias no tecido subcutâneo, com presença de sangue não coagulado e em boa parte associadas a edema (edema hemorrágico), que se estendia desde o local da inoculação até as regiões cervical, torácica, escapular e membro. Na periferia das áreas hemorrágicas havia predominantemente edema gelatinoso. Havia ainda presença de grande quantidade de líquido sanguinolento nas cavidades torácica, pericárdica e abdominal. Não foram encontradas alterações histológicas significativas.

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Section: Quadro Clínico Geralunclassified

Aspectos clínico-patológicos do envenenamento botrópico experimental em equinos

et al. 2011

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“…Em humanos envenenados por serpentes do antigo gênero Bothrops (França & Málaque 2003) e em camundongos, ratos (Melo & Suares-Kurtz 1987, Santos et al 1992, Calil-Elias et al 2002, Sifuentes et al 2008) e bovinos inoculados experimentalmente ), os níveis de CK também estavam aumentados. Em humanos, França & Málaque (2003) verificaram que CK e DHL também encontravam-se aumentadas em pacientes com processo inflamatório no local da picada.…”

Section: Resultados Laboratoriaisunclassified

Envenenamento experimental por Bothropoides jararaca e Bothrops jararacussu em ovinos: aspectos clínico-patológicos e laboratoriais

et al. 2010

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The purpose of this study was to establish the clinic-pathological and laboratory changes in sheep inoculated with Bothropoides jararaca and Bothrops jararacussu venom to provide subsidies for the differential diagnosis of snake bites. The liofilized venoms were diluted in 1 ml saline and administrated subcutaneously to four sheep. Three of the animals died, and the one that received 0.5mg/kg (B. jararaca venom) recovered. First symptoms were observed from 7 minutes to 1 hour after inoculation, and the clinical course varied from 7 hours and 9 minutes to 21 hours and 59 minutes. The symptoms, independent of the dosage, were swelling of the inoculation site, increased bleeding time and capillary filling, tachycardia, dyspnea, pale mucous membranes and diminished reaction to external stimuli. Laboratory tests revealed pronounced normocytic and normochromic anemia, trombocytopenia, slight reduction of fibrogen and total plasmatic protein, in two animals diminished hematocrit, besides pronounced increase of creatinaquinase and lactic dehydrogenase. At necropsy, the main findings at the inoculation site and adjacent tissues were extensive hemorrhages in the sheep inoculated with jararaca venom, and predominantly edema in the two animals inoculated with jararacussu venom. In two sheep which received jararacussu venom, acute pulmonary edema was observed. Hemorrhage and edema as the main histopathological changes, besides necrosis of muscle fibers and vessels at the inoculation site and adjacent tissue was observed. The renal tubular necrosis was attributed to shock. The volume increase at the inoculation site and surroundings was mainly due to hemorrhage (B. jararaca) or edema (B. jararacussu).

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“…They were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) and then injected with crude venom of Bothrops leucurus (BlV) 1.0 mg/kg in PSS by applying 50 μ L of the solution next to the extensor digitorum longus (EDL) muscle of the right hind limb (EDL perimuscular injection, in order to prevent direct mechanical damage to the muscle), as described previously [9, 17]. Mice treated with A. cochliacarpos received administration by oral gavage.…”

Section: Methodsmentioning

confidence: 99%

Abarema cochliacarposExtract Decreases the Inflammatory Process and Skeletal Muscle Injury Induced byBothrops leucurusVenom

Saturnino-Oliveira

Santos

Guimarães

et al. 2014

BioMed Research International

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Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

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Effect of perimuscular injection of Bothrops jararacussu venom on plasma creatine kinase levels in mice: influence of dose and volume

Cited by 20 publications

References 8 publications

Aspectos clínico-patológicos do envenenamento botrópico experimental em equinos

Aspectos clínico-patológicos do envenenamento botrópico experimental em equinos

Envenenamento experimental por Bothropoides jararaca e Bothrops jararacussu em ovinos: aspectos clínico-patológicos e laboratoriais

Abarema cochliacarposExtract Decreases the Inflammatory Process and Skeletal Muscle Injury Induced byBothrops leucurusVenom

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