Effects of 60Co gamma radiation on crotamine

Boni-Mitake, Malvina; Costa, Hilton Nobre da; Spencer, Patrick Jack; Vassilieff, V.S.; Rogero, J.R.

doi:10.1590/s0100-879x2001001200004

Cited by 35 publications

(23 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This agrees with the findings of other authors (7,12,21,(31)(32)(33)(34)(35)(36)(37)(38)42), which assured that ionizing radiation has the property to attenuate venoms toxicity without altering immunological properties.…”

Section: Discussionsupporting

confidence: 92%

“…The principal physical agents are: x-ray radiation (19), ultraviolet radiation (46), heat (15), and gamma radiation (25). The latter, according to previous works (1,7,9,12,14,17,21,(31)(32)(33)(34)(35)(36)(37)(38)(41)(42)(43), has proved to be an excellent tool of detoxification, since it is able to reduce toxicity without altering immunogenicity. In addition, this radiation does not add any new substance to the venom.…”

Section: Introductionmentioning

confidence: 91%

See 1 more Smart Citation

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira¹,

Nascimento²,

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated ( 60 Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD 50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60 th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60 Coirradiation. The sera produced with native venom showed neutralizing potency and capacity similar to those of the sera produced with irradiated venom. All antibodies were able to neutralize five LD 50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom, however, necrosis and death occurred in animals inoculated with native venom.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 91%

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira¹,

Nascimento²,

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…Indeed, the lethality of unfolded crotamine was shown to be reduced by approximately 50%. 12 Interestingly, crotamine appears to be active against only a narrow spectrum of bacteria, in contrast with most vertebrate b-defensins that are broadly active. This is perhaps not surprising, given a great degree of diversity in the primary amino-acid sequence between crotamine and b-defensins (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

“…The crotalic venom from the snake Crotalus durissus terrrificus was purchased from CEVAP, Botucatu, São Paulo, Brazil, and used to purify crotamine as described, 12 with slight modifications. Briefly, crotamine was purified from crude venom by size exclusion on a Sephacryl S200 column (GE Healthcare, Uppsala, Sweden), followed by cation exchange chromatography on a 1-ml Resource S on FPLC system (Akta Purifier System, GE Healthcare).…”

Section: Purification and Reduction Of Crotaminementioning

confidence: 99%

In vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus

et al. 2011

View full text Add to dashboard Cite

Crotamine, a myotoxin from the venom of South American rattlesnake, is structurally related to b-defensins, antimicrobial peptides (AMPs) found in vertebrate animals. Here, we tested the antibacterial properties of crotamine and found that it killed several strains of Escherichia coli, with the MICs ranging from 25 to 100 lg ml À1 . Time-kill and bacterial membrane permeabilization assays revealed that killing of bacteria by crotamine occurred within 1 h and reached the maximum by 2 h. Additionally, the anti-E. coli activity of crotamine was completely abolished with 12.5 mM NaCl. Furthermore, the three intramolecular disulfide bonds of crotamine appeared dispensable for its antibacterial activity. The reduced form of crotamine was active against E. coli as well. However, crotamine showed no or weak activity up to 200 lg ml À1 against other species of Gram-negative and Gram-positive bacteria. Crotamine showed no appreciable hemolytic activity to erythrocytes. Our studies revealed that crotamine is also an AMP that kills bacteria through membrane permeabilization. However, crotamine appears to have a narrow antibacterial spectrum, distinct from many classical b-defensins, reinforcing the notion that crotamine originated from the b-defensin gene lineage, but has undergone significant functional diversification.

show abstract

“…The activation of snake venoms occurs under the influence of some physical factors, for example, under the influence of ultraviolet irradiation and X-rays [12]. So, in 7 days after a venom irradiation of Filipina cobras by γ radiation ( 60 Со) in doses 0.25, 0.5, 1 mrad, toxicity of snake venom made 83, 66 and 43 %, accordingly from not irradiated venom [16,17,18]. On literary data snake venom in the dried up kind keeps pharmacological and toxic properties till 22 years and even more.…”

Section: Imentioning

confidence: 99%