Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12

Abstract

DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.

Pseudogene INTS6P1 regulates its cognate gene INTS6 through competitive binding of miR-17-5p in hepatocellular carcinoma

“…Several studies have suggested that INTS6 plays tumor suppressive roles in several human cancers, such as non-small cell lung carcinoma, esophageal squamous cell carcinoma and prostate carcinoma [20-23, 31-34]. Mechanistically, INTS6 appears to induce Gap 1 (G1) arrest, explaining, in part its tumor suppressive roles in prostate cancer [20].…”

Use of Degenerate Primers and Touchdown PCR for Construction of cDNA Libraries

“…The percent of nonredundancy is shown in relation to the cumulative number of sequences obtained for library F24I (squares), F33 (triangles), and H8-1 (circles). clusters that vary in size from 10 to 324 members (2). Unfortunately, there are only 1 4 000 S. mansoni EST sequences deposited in GenBank, and it would be difficult to determine if the modifications introduced here affect the ability of low-stringency RT-PCR to amplify rare S. mansoni messages.…”

“…In fact, despite the large set of more than 3.7 million EST sequences currently available from human tissues, it is apparent that only a fraction of the human gene complement has its full sequence determined. In this respect, a new low-stringency RT-PCR EST (ORESTES) sequencing strategy was shown to be complementary to other transcript sequencing strategies and to help determine the complete set of human expressed genes (2). A single arbitrary nondegenerate oligonucleotide primer is used both in the reverse transcription step and in the subsequent lowstringency PCR.…”