The 9-O-acetyl GD3 gangliosides are expressed by migrating chains of subventricular zone neurons in vitro

Miyakoshi, L.M.; Mendez-Otero, Rosália; Hedin-Pereira, Cecília

doi:10.1590/s0100-879x2001000500016

Cited by 22 publications

(24 citation statements)

References 18 publications

(12 reference statements)

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“…In these regions, cells are found with migratory profiles. The observation that labeling with Jones mAb is restricted to regions of cell migration is consistent with previous reports that have suggested that the ganglioside 9-O-acetyl GD3 is a marker for neuronal migration in the developing Schlosshauer et al, 1988;Mendez-Otero and Ramon-Cueto, 1994;Miyakoshi et al, 2001;Santiago et al, 2001) (for review, see Mendez-Otero and Santiago, 2001) and adult (Mendez-Otero and Cavalcante, 1996) nervous system. We also show that the antibody against 9-Oacetyl GD3 arrests granule cells in the external granular cell layer and diminishes the number of BrdU-positive granule cells in the internal granular cell layer by 57%.…”

Section: Discussionsupporting

confidence: 90%

Immunoblockage of 9-O-Acetyl GD3 Ganglioside Arrests theIn VivoMigration of Cerebellar Granule Neurons

Santiago¹,

Costa²,

Mendez-Otero³

2004

J. Neurosci.

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Section: Discussionsupporting

confidence: 90%

Immunoblockage of 9-O-Acetyl GD3 Ganglioside Arrests theIn VivoMigration of Cerebellar Granule Neurons

Santiago¹,

Costa²,

Mendez-Otero³

2004

J. Neurosci.

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“…However, 9-O-acGD3 molecules were also shown to be expressed in the developing and adult SVZ/RMS labeling migratory profiles (41). Their role as possible mediators of neuroblast homophilic migration was confirmed in studies in which ganglioside 9-O-acGD3 was shown to be present in migrating chains of cells devoid of glia in postnatal SVZ explant cultures (42). Immunoblocking ganglioside 9-O-acGD3 produced a drastic decrease in the migration halo in these explants (43).…”

Section: Cell Contact-mediating Moleculesmentioning

confidence: 86%

“…Immunoblocking ganglioside 9-O-acGD3 produced a drastic decrease in the migration halo in these explants (43). One could speculate that PSA-NCAM and 9-O-acGD3 would be expressed in different subsets of the SVZ cells but in general both molecules are found in the same cells (42). Recent evidence suggests that these gangliosides might reside in focal adhesion sites together with integrins and other adhesion-mediating molecules performing a still obscure role.…”

Section: Cell Contact-mediating Moleculesmentioning

confidence: 99%

Cell migration in the postnatal subventricular zone

Menezes

Marins

Alves

et al. 2002

Braz J Med Biol Res

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New neurons are constantly added to the olfactory bulb of rodents from birth to adulthood. This accretion is not only dependent on sustained neurogenesis, but also on the migration of neuroblasts and immature neurons from the cortical and striatal subventricular zone (SVZ) to the olfactory bulb. Migration along this long tangential pathway, known as the rostral migratory stream (RMS), is in many ways opposite to the classical radial migration of immature neurons: it is faster, spans a longer distance, does not require radial glial guidance, and is not limited to postmitotic neurons. In recent years many molecules have been found to be expressed specifically in this pathway and to directly affect this migration. Soluble factors with inhibitory, attractive and inductive roles in migration have been described, as well as molecules mediating cell-to-cell and cell-substrate interactions. However, it is still unclear how the various molecules and cells interact to account for the special migratory behavior in the RMS. Here we will propose some candidate mechanisms for roles in initiating and stopping SVZ/RMS migration.

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“…Neutrophil motility is sharply reduced when GM1 gangliosides are neutralized (35). Migrat- ing neurons in the developing cerebral cortex, subventricular zone, or cerebellum are also affected after immunoblockage of 9-O-acetyl GD3 ganglioside (11,13,14). Gangliosides are known to interact laterally in the membrane, regulating the responsiveness of receptors for insulin, epidermal growth factor, and vascular endothelial growth factor (36).…”

Section: Discussionmentioning

confidence: 99%

Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells*

Ribeiro-Resende

Ribeiro-Guimarães

Lemes

et al. 2010

Journal of Biological Chemistry

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Mycobacterium leprae (ML), the etiologic agent of leprosy, mainly affects the skin and peripheral nerves, leading to demyelization and loss of axonal conductance. Schwann cells (SCs) are the main cell population infected by ML in the nerves, and infection triggers changes in the SC phenotype from a myelinated to a nonmyelinated state. In the present study, we show that expression of 9-O-acetyl GD3, a ganglioside involved in cellular anti-apoptotic signaling and nerve regeneration, increases in SCs following infection with ML. Observation by confocal microscopy together with coimmunoprecipitation suggested that this ganglioside participates in ML attachment and internalization by SC. Immunoblockage of 9-O-acetyl GD3 in vitro significantly reduced adhesion of ML to SC surfaces. Finally, we show that activation of the MAPK (ERK 1/2) pathway and SC proliferation, two known effects of ML on SCs that result in demyelization, are significantly reduced when the 9-O-acetyl GD3 ganglioside is immunoblocked. Taken together, these data suggest the involvement of 9-O-acetyl GD3 in ML infection on SCs.Leprosy, one of the oldest recorded diseases, remains an important cause of morbidity, with ϳ250,000 new cases/year. Mycobacterium leprae (ML), 2 the causative agent of leprosy, is an obligate intracellular pathogen with high tropism for Schwann cells (SCs) (1). Data collected over the past 15 years suggest that cell surface molecules are involved in ML adhesion to SCs (2). The G domains of the laminin-␣2 chain and the dystroglycan receptor have been shown to play roles in mediating ML attachment to the SC surface (3). Phenolic glycolipid-I, a major unique glycoconjugate on the ML surface, binds laminin-2, which explains the predilection of the bacterium for peripheral nerves (4). In vitro and in vivo studies in Rag-1 Ϫ/Ϫ mice, which lack B and T lymphocytes, showed that ML attachment to the SC surface is sufficient to cause demyelination in peripheral nerves (5). This effect was found to be dependent on neuregulin receptor, ErbB-2, and ERK 1/2 activation by ML, leading to MAPK signaling and proliferation (6). Additionally, conversion of SCs from the myelinated to nonmyelinated phenotype is directly related to increased proliferative capacity (7). Tapinos et al. (8) have shown that ML is able to induce SC proliferation through ERK 1/2 activation via MEK-independent and p56LcK-dependent pathways.9-O-Acetyl GD3 ganglioside is an acetylated glycolipid present in the cell membrane of many types of vertebrate cells (9). This molecule plays an important role in the development, differentiation, and regeneration of the nervous system. Among its multiple functions are: cell attachment to the extracellular matrix, neuronal migration, neurite outgrowth in vitro, and control of apoptosis (10 -17). 9-O-Acetyl GD3 ganglioside is highly expressed after sciatic nerve crush in the regenerating nerve, as well as in the dorsal root ganglia and lumbar spinal cord. During peripheral nerve regeneration, 9-O-acetyl GD3 is mainly expressed by SCs, wh...

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The 9-O-acetyl GD3 gangliosides are expressed by migrating chains of subventricular zone neurons in vitro

Cited by 22 publications

References 18 publications

Immunoblockage of 9-O-Acetyl GD3 Ganglioside Arrests theIn VivoMigration of Cerebellar Granule Neurons

Immunoblockage of 9-O-Acetyl GD3 Ganglioside Arrests theIn VivoMigration of Cerebellar Granule Neurons

Cell migration in the postnatal subventricular zone

Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells*

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