A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins

Abstract

Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10 5 ) and CHO-745 (2 x 10 5 and 5 x 10 5 ) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications.

A Xylogalactofucan from the Brown SeaweedSpatoglossum schröederiStimulates the Synthesis of an Antithrombotic Heparan Sulfate from Endothelial Cells

“…Endothelial cells proliferation and [ 3 H]-thymidine incorporation in the presence of fucan C (100 mg/mL) were measured as previously described [5], [11].…”

“…Fucans have been demonstrated to exhibit anticoagulant [2], [3], imunomodulatory, antitumoral [4], and antiadhesive [5] activities. The structural characteristics of sulfated fucans with these biological activities have not been determined yet; consequently, the structure-activity relationships have still to be elucidated [1], [3].…”

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

“…Algal fucans have anticoagulant/antithrombotic (5,8,9), anticomplement (10), antiproliferative (11), antiviral (12), and antiadhesive activities (13). However, the relationship between structure and biological activity of fucans has not been fully elucidated.…”

Fucan Inhibits Chinese Hamster Ovary Cell (CHO) Adhesion to Fibronectin by Binding to the Extracellular Matrix

“…Adhesion assay Cells (2 10 5 or 5 10 5 final concentration/well) were added to FN coated wells and allowed to attach for 1 h at 37 8C under a 2.5 % CO 2 atmosphere in the absence (control) or presence of fucan B (final concentration from 0.01 mg to 1 mg/mL) for 1 h and the adhesion assay was performed as previously described [6]. Each experiment was executed in triplicate.…”

“…The results suggest that the fucan shows anti-adhesive activity by binding directly to FN, and blocking FN sites that are recognized by cell surface ligands, possibly the integrin family. mL) inhibited both CHO wild-type and mutant cell adhesion to several ECM proteins, mainly fibronectin (FN) [6].…”