Apoptosis of sensory neurons and satellite cells after sciatic nerve transection in C57BL/6J mice

Oliveira, Alexandre Leite Rodrigues de

doi:10.1590/s0100-879x2001000300012

Cited by 19 publications

(10 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The facts reported above are in line with other works dealing with various isogenic strains of mice showing variations in the regenerative capacity after crushing or sectioning of the sciatic nerve (Lainetti et al, 1995;Lu et al, 1994;Oliveira and Langone, 2000;Oliveira, 2001;Xin et al, 1990). In this sense, C57BL/6J number of regenerated axons, 2 weeks after axotomy, was one-fifth of that in A/J.…”

Section: Introductionsupporting

confidence: 90%

MHC I expression and synaptic plasticity in different mice strains after axotomy

Sabha

Emirandetti

Cullheim

et al. 2007

Synapse

View full text Add to dashboard Cite

The success of axonal regeneration has been attributed to a co-operation between the severed neurons and the surrounding environment, including non-neuronal cells and the extracellular matrix. Important differences regarding the regeneration potential after injury have been described among inbred mice strains. To date, there is only limited knowledge of how such variation can be linked with the genetic background. It has recently been demonstrated that MHC class I molecules have an influence on the spinal cord synaptic plasticity elicited by a peripheral lesion, and the regenerative capacity following such a lesion. Therefore, in the present work we compared the MHC I expression after axotomy in three isogenic mice strains, namely C57BL/6J, Balb/cJ, and A/J, and investigated the fine ultrastructure of the synaptic elimination process that follows such lesion. The results show that C57BL/6J mice, that have a comparatively poor regenerative potential, display a lower upregulation of MHC I in the spinal cord, coupled with a slower synaptic stripping. On the other hand, A/J mice, which have been shown to have a stronger axonal regrowth potential, showed a clear upregulation of MHC I and a sharp acute loss of afferents, at 1 week after lesion. Our results suggest that a more prominent expression of MHC I in the first week after lesion may positively influence the regenerative outcome associated with a more effective axonal regrowth.

show abstract

Section: Introductionsupporting

confidence: 90%

MHC I expression and synaptic plasticity in different mice strains after axotomy

Sabha

Emirandetti

Cullheim

et al. 2007

Synapse

View full text Add to dashboard Cite

show abstract

“…By cross-comparing our gene list with up-regulated genes in a model of pre-conditioned DRG32 as well as known RAGs (Supplementary Table S6), we uncover additional novel RAGs in our injury model via scRNA-seq. These novel RAG genes are connected by the known RAGs genes such as Atf3, c-Jun, Sox11 and Klf6 in regeneration gene network analysis as well4546474849.…”

Section: Discussionmentioning

confidence: 99%

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Huang

et al. 2016

Sci Rep

122

111

View full text Add to dashboard Cite

Peripheral nerve injury leads to various injury-induced responses in sensory neurons including physiological pain, neuronal cell death, and nerve regeneration. In this study, we performed single-cell RNA-sequencing (scRNA-seq) analysis of mouse nonpeptidergic nociceptors (NP), peptidergic nociceptors (PEP), and large myelinated sensory neurons (LM) under both control and injury conditions at 3 days after sciatic nerve transection (SNT). After performing principle component and weighted gene co-expression network analysis, we categorized dorsal root ganglion (DRG) neurons into different subtypes and discovered co-regulated injury-response genes including novel regeneration associated genes (RAGs) in association with neuronal development, protein translation and cytoplasm transportation. In addition, we found significant up-regulation of the genes associated with cell death such as Pdcd2 in a subset of NP neurons after axotomy, implicating their actions in neuronal cell death upon nerve injury. Our study revealed the distinctive and sustained heterogeneity of transcriptomic responses to injury at single neuron level, implicating the involvement of different gene regulatory networks in nerve regeneration, neuronal cell death and neuropathy in different population of DRG neurons.

show abstract

“…Studies have demonstrated that this condition causes structural, electrophysiological, molecular and metabolic changes in mammalian dorsal root ganglia (DRG) cells and spinal cord. These changes include progressive loss of cells (Oliveira, 2001;Jiang and Jakobsen, 2010), proliferation and activation of satellite glial cells (Hanani, 2005) and reorganisation of the metabolic priorities of neural cells (Enes et al, 2010). There is evidence that the transection of facial and hypoglossal nerves produces a significant increase in glucose utilisation in motor nuclei of these nerves in rats (Kreutzberg and Emmert, 1980).…”

Section: Introductionmentioning

confidence: 99%

Effects of sciatic nerve transection on glucose uptake in the presence and absence of lactate in the frog dorsal root ganglia and spinal cord

et al. 2014

View full text Add to dashboard Cite

Frogs have been used as an alternative model to study pain mechanisms because the simplicity of their nervous tissue and the phylogenetic aspect of this question. One of these models is the sciatic nerve transection (SNT), which mimics the clinical symptoms of "phantom limb", a condition that arises in humans after amputation or transverse spinal lesions. In mammals, the SNT increases glucose metabolism in the central nervous system, and the lactate generated appears to serve as an energy source for nerve cells. An answerable question is whether there is elevated glucose uptake in the dorsal root ganglia (DRG) after peripheral axotomy. As glucose is the major energy substrate for frog nervous tissue, and these animals accumulate lactic acid under some conditions, bullfrogs Lithobates catesbeianus were used to demonstrate the effect of SNT on DRG and spinal cord 1-[ C-2-DG uptake was lower when lactate was added to the incubation medium. No change was found in glucose and lactate oxidation after SNT, but lactate and glucose levels in the blood were reduced. Thus, our results showed that SNT increased the glucose metabolism in the frog DRG and spinal cord. The effect of lactate on this uptake suggests that glucose is used in glycolytic pathways after SNT. Efeito da secção do nervo isquiático sobre a captação de glicose na presença e ausência de lactato em gânglio da raiz dorsal e medula espinhal de rãs ResumoAs rãs são usadas como modelos experimentais alternativos no estudo da nocicepção, tanto pela simplicidade do seu tecido nervoso como por permitirem uma abordagem filogenética sobre o tema. Um desses modelos é a secção do nervo isquiático (SNI), o qual simula os sintomas clínicos do "membro fantasma", uma condição que ocorre nos humanos após amputação ou secção completa da medula espinal. Em mamíferos, a SNI aumenta o metabolismo da glicose no sistema nervoso central, e o lactato é uma fonte energética para as células nervosas. Porém é desconhecido se essa é a situação em gânglio da raiz dorsal (GRD). Como a glicose é o principal substrato energético para o tecido nervoso de rãs, e a concentração plasmática de lactato está aumentada nesses animais em distintas situações, a rã-touro Lithobates catesbeianus foi usada para demonstrar os efeitos da SNI sobre a captação de 1-[ 14 C] 2-deoxi-D-glicose ( 14 C-2-DG), na presença e ausência de lactato, em GRD e medula espinal. Foram demonstrados ainda os efeitos dessa condição experimental sobre a formação de 14 CO 2 a partir de 14 C-glicose e 14 C-L-lactato, e a concentração plasmática de glicose e lactato. A captação de 3-O-[14 C] metil-D-glicose ( 14 C-3-OMG) foi usada para demonstrar a relação tecido/meio estável da glicose nessas condições. A captação de 14 C-2-DG aumentou três dias após a SNI, sem qualquer alteração na captação de 14 C-3-OMG. O aumento foi reduzido quando o lactato foi acrescentado ao meio de incubação. A taxa de oxidação da glicose e do lactato não modificou após SNI, mas houve redução na concentração plasmática de glicose e lactato. Ass...

show abstract

Apoptosis of sensory neurons and satellite cells after sciatic nerve transection in C57BL/6J mice

Cited by 19 publications

References 16 publications

MHC I expression and synaptic plasticity in different mice strains after axotomy

MHC I expression and synaptic plasticity in different mice strains after axotomy

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Effects of sciatic nerve transection on glucose uptake in the presence and absence of lactate in the frog dorsal root ganglia and spinal cord

Contact Info

Product

Resources

About