Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells

Abstract

Prostaglandins are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells is well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A 1 (PGA 1 ; 0.25, 1.25 and 12.5 µg/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA 1 stimulates the synthesis of several polypeptides with molecular masses of 87, 80, 70, 57, 29, 27 and 23 kDa in Aedes albopictus cells. When the proteins induced by PGA 1 and those induced by heat treatment were compared by polyacrylamide gel electrophoresis, PGA 1 was found to induce the stress proteins. The HSP70 family and the low-molecular weight polypeptides (29 and 27 kDa, respectively) were induced by PGA 1 in the lag phase. We also observed that PGA 1 is able to induce a 23-kDa polypeptide independently of the growth phase of the cell.

Prostaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL-HzAM1 cells)

“…folding were influenced to a greater or lesser extent by PG treatments, including proteins 1, 16, 25, 26 and 28. This is consistent with the biomedical background, in which PGs are thought to play major signaling roles in stress responses, including induction of HSP genes (Negishi and Katoh, 2002 (Barbosa and Rebello, 1998). Our experimental PG treatments influenced expression of genes encoding proteins involved in the signal transduction category, #32, 33 and 36.…”

Prostaglandina A1 inibe a replicação do vírus Sindbis em células de rim de macaco e em células de mosquito

“…PGA 1 induces the synthesis stress proteins in Vero cells (FIGURE 2) and in A. albopictus cells (BARBOSA; REBELLO, 1998). We also found that in SINV infected cells, heat treatment (39 ºC for Vero cells and 30 ºC for A. albopictus) inhibited virus replication by 98% in both cell lines.…”

Cell Line Platforms Support Research into Arthropod Immunity

“…PGs are synthesized in vertebrates by cyclooxygenases, although, in insects, the enzymes have been recently identified as peroxidases [97,98]. Barbosa and Rebello [51] reported that prostaglandin A1 (PGA 1 ) mediates the synthesis of stress proteins during cells' lag phase (specifically, HSPs 27, 29, 70, 80, and 87 kDa) in the C6/36 Ae. albopictus cell line.…”