Image analysis and definition of nuclear phenotypes

Vidal, Benedicto de Campos

doi:10.1590/s0100-84551997000400031

Cited by 8 publications

(5 citation statements)

References 9 publications

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“…5). As regards Vidal's [15,16] scatter diagram relating Sc% vs AAR, part of the distribution of the points representing BP1-Tras cell nuclei differed from the distribution for nuclei of BP1 cells (Fig. 6) and for previously reported data on BP1-E1 cells [17] (Fig.…”

Section: Resultsmentioning

confidence: 56%

“…The parameters obtained were those which permitted us to plot a scatter diagram as proposed by Vidal [15], relating Sc% (nuclear relative area covered with stained chromatin presenting optical density values above a selected threshold level, in this case absorbance 0.200) vs AAR (a dimensionless parameter that expresses how many times the average absorbance of the "condensed" chromatin exceeds that of the entire nucleus). AAR = (Ac/Sc)/(A T /S T ), where Ac, "condensed" chromatin Feulgen-DNA values; A T , nuclear Feulgen-DNA values; Sc, "condensed" chromatin stained area; S T , nuclear stained area [16].…”

Section: Scanning Microspectrophotometry Image Analysismentioning

confidence: 99%

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Ha-rasOncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells

Mello

Vidal

Russo

1999

Analytical Cellular Pathology

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When transfected to benzo[a]pyrene (BP)‐transformed MCF‐10F human breast epithelial cells (BP1 cell line) the c‐Ha‐ras oncogene has proven to enhance the neoplastic changes initiated by exposure to BP, giving rise to an aggressive tumorigenic cell line, BP1‐Tras. We have previously demonstrated by image analysis that BP affects the DNA content and the chromatin supraorganization of MCF‐10F cells. Here Feulgen‐stained BP1‐Tras cells were studied by image analysis in order to evaluate possible additional changes in DNA content and chromatin texture induced by insertion of the ras oncogene. A high variability in DNA content also including polyploidy or near‐polyploidy, and an increase in the packing states of the chromatin which became still condensed in BP1 cells were found in BP1‐Tras cells. The results differed from those reported for the BP1‐E1 cell line which is also an aggressive tumorigenic cell line, but was attained through progressive passages of BP‐transformed cells. It was demonstrated that different patterns of changes in DNA content and chromatin organization may be involved in equally aggressive tumorigenic BP‐transformed cell lines originated from the same cell line by different mechanisms.

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Section: Resultsmentioning

confidence: 56%

Section: Scanning Microspectrophotometry Image Analysismentioning

confidence: 99%

Ha-rasOncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells

Mello

Vidal

Russo

1999

Analytical Cellular Pathology

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“…Video image analysis cytometry was carried out according to Vidal (1997), using a Global Lab Image software (Data Translation, Inc. System, Marlboro, MA, USA) coupled to a Zeiss photomicroscope equipped with a PolNeofluar 25/0.60 objective, 1.25 optovar, 1.4 condenser, λ = 546 nm, and a 12-v 60-w lamp connected to a stabilizer. The images to be processed were transmitted from the microscope to the computer by a JVC monochromatic CCD video camera.…”

Section: Image Analysismentioning

confidence: 99%

Image analysis of DNA fragmentation and loss in V79 cells under apoptosis

2000

Self Cite

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Nuclear image analysis of Feulgen-stained V79 fibroblasts after three days in culture was used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as the Feulgen-DNA content, degree of chromatin condensation and nuclear areas, in association with visual morphology. The fibroblasts were initially plated at a density of 10(5) cells/ml and incubated under optimal culture conditions without subculturing. Following confluency, the cells underwent contact inhibition apoptosis. Image analysis revealed three nuclear phenotypes which were defined in terms of their morphological characteristics and levels of chromatin condensation. A decrease in the amount of Feulgen-DNA was detected in apoptotic cells and in cells suspected of undergoing apoptosis. This decrease was assumed to indicate DNA loss. Image analysis procedures may therefore provide a useful tool for discriminating cells in the early stages of apoptosis.

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“…They are used to extract information contained in images by detecting, diagnosing, and describing the texture and geometry of the images. 12 The nuclei, being the vital structure in image analysis, mandate the use of certain special nuclear stains to enhance their study. Feulgen is a special stain that binds stiochiometrically with DNA molecule and, hence, becomes indispensable in the study.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Nuclear Morphometry in Oral Squamous Cell Carcinoma: A Retrospective Study

Swain¹,

Patel²,

Pathak³

et al. 2017

Journal of Contemporary Dentistry

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Aim: To evaluate and compare various nuclear morphometric parameters in different histopathological grades of oral squamous cell carcinoma (OSCC) by using computerized image analysis and also to correlate it with regional lymph node metastasis. Materials and methods:This retrospective study was conducted on paraffin blocks of 40 tissue specimens of OSCC cases treated with neck dissection, which were retrieved from the archives of the Department of Oral Pathology and Microbiology, Mahatma Gandhi Mission's Dental College and Hospital, Navi Mumbai, India. All cases were histopathologically graded as well, moderately, and poorly differentiated OSCC. Further, they were also categorized based on pathological lymph node status as with or without lymph node metastasis. Sections from tumor proper were subjected to Feulgen nuclear staining technique. Images of 10 microscopic fields at the deepest invading part of tumor were captured randomly and 100 nuclei of tumor cells with clear, complete, nonoverlapping outlines were selected in each case. Nuclear morphometric parameters, such as large diameter, small diameter, nuclear area, and nuclear perimeter were measured for each of the 100 cells.Results: An increase in mean nuclear area coefficient of variation (NACV) was observed in OSCC cases with lymph node metastasis pN(+) than in those without lymph node metastasis pN(-), (p = 0.67). A significant increase in nuclear area and perimeter was observed in pN(+) cases (p < 0.01). A significant decrease in circular rate and increase in largest to smallest nuclear diameter (L/S) ratio (p < 0.01) was observed in pN(+) cases. On comparing the nuclear morphometric parameters with different histopathological grades of OSCC, we found an increase in mean NACV values from well-differentiated OSCC to moderately differentiated and poorly differentiated OSCC significant difference (LSD) post hoc test, indicated a difference only between well-differentiated and poorly differentiated cases of OSCC (p = 0.132). Conclusion:Our observations reveal that tumor cells with greater nuclear dimension and more elliptical shape tend to show increased incidence of nodal metastasis. Also, a positive inclination was observed in nuclear size and shape with increasing histopathological grades of OSCC. However, our data warrant a large-scale study to establish nuclear morphometry as a quantitative objective parameter and also for the rational application of the same.

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Image analysis and definition of nuclear phenotypes

Cited by 8 publications

References 9 publications

Ha-rasOncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells

Ha-rasOncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells

Image analysis of DNA fragmentation and loss in V79 cells under apoptosis

Evaluation of Nuclear Morphometry in Oral Squamous Cell Carcinoma: A Retrospective Study

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