Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

Alzate-Marin, Ana Lília; Baía, Gilson S.; Filho, Sebastião Martins; Júnior, Trazilbo José de Paula; Sediyama, Carlos Sigueyuki; Barros, Everaldo Gonçalves de; Moreira, Maurílio Alves

doi:10.1590/s0100-84551996000400016

Cited by 14 publications

(11 citation statements)

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“…To ensure that the F 1 seeds were true hybrids, we analyzed the color, shape, size and brightness of the corresponding F 2 seeds. In the case of the cross between 'Ouro Negro' and 'Cornell 49-242', which are phenotypically very similar, the F 1 seeds were submitted to a molecular analysis with marker SF10 1050a (Corrêa et al, 2000) according to a procedure described by Alzate-Marin et al (1996). The F 2 segregating populations were used in artificial inoculations.…”

Section: Inheritance Studies and Allelism Testsmentioning

confidence: 99%

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Sanglard¹,

Ribeiro²,

Balbi³

et al. 2013

JAS

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Angular leaf spot (ALS), incited by the fungus Pseudocercospora griseola, is a major disease affecting common bean. Important resistance sources have been identified and characterized: AND 277, BAT 332, Cornell 49-242, MAR-2, and Mexico 54. P. griseola presents high physiological variability. The control of this kind of pathogen requires continuous evaluation, including monitoring and characterization of pathogenic variability, and eventual germplasm introgression of genes in adapted cultivars. Cultivar Ouro Negro is currently being used as an ALS resistance source in Brazil. This work aimed to characterize the ALS resistance gene present in cultivar Ouro Negro in relation to the other five resistance sources. These cultivars were crossed with 'Ouro Negro' (allelism tests) and with susceptible cv. Rudá (inheritance studies) and their segregation patterns were evaluated. The inheritance studies and the allelism tests indicate that 'Ouro Negro' harbors at least one resistance locus which is distinct from those present in the other resistance sources.

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Section: Inheritance Studies and Allelism Testsmentioning

confidence: 99%

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Sanglard¹,

Ribeiro²,

Balbi³

et al. 2013

JAS

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“…For those crosses where it was not possible to use these phenotypic markers, DNA markers were used to distinguish the hybrid from the selfing. The DNA extraction was made according to the methodology proposed by Doyle and Doyle (1990), and the RAPD technique was used for the identification of hybrid plants ( Alzate-Marin et al, 1996). The F 1 hybrids resulting from the cross Uberaba x Bossier were identified using the OPB-03 or OPAG-10 RAPD primers, and the F 1 hybrids from Kent x Lincoln with the AB-4 RAPD primer or microssatellite AN1.…”

Section: Cultivars and Crossesmentioning

confidence: 99%

Multivariate analysis of combining ability for soybean resistance to Cercospora sojina Hara

et al. 2004

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Seven soybean cultivars (Bossier, Cristalina, Davis, Kent, Lincoln, Paraná and Uberaba), with different levels of resistance to Cercospora sojina, race 04, were crossed according to a diallel design, with no reciprocals, to determine the general and the specific combining abilities for the resistance. The evaluations of the reaction to the disease were performed 20 days after the inoculation of the fungus on the most infected leaflet of the plant, in the parents and in the F1 hybrids. To quantify the resistance, the following characteristics were evaluated: infection degree (ID); number of lesions per leaflet (NLL); lesion mean diameter (LMD); lesioned leaf area (LLA); percentage of lesioned leaf area (PLLA); number of lesions per square centimeter (NLC) and disease index (DI). The relative importance of each characteristic was evaluated by the canonical variables analysis and the LLA and NLL characteristics were eliminated from the multivariate function. With the remaining five characteristics, a multivariate index was created using the first canonical vector, which was submitted to the diallel analysis, according to Griffings fixed model, method 2. The most important characters to discriminate resistant from susceptible soybean plants to C. sojina were: ID, LMD, NLC, DI and PLLA. Cristalina, Davis and Uberaba cultivars are the best ones among those tested that can be recommended as parents in soybean breeding programs seeking resistance to Cercospora sojina. The additive, dominant and epistatic genetic effects were important for the expression of the resistance, although the additive genetic effect was the most important component.

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“…Thus, the use of molecular tools that can enhance or monitor this process is particularly important. Alzate-Marin et al (1996) have already demonstrated the efficiency of RAPD (Random Amplified Polymorphic DNA) markers for the confirmation of biparental crosses between genetically close parents of common bean and soybean without phenotypic contrast in readily assessable morphological traits. Another important stage of plant breeding programs is the confirmation of the identity and genetic purity of the genotypes during the seed production process, which can also be monitored or improved with molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

Morais

Vieira

Almeida

et al. 2016

Crop Breed. Appl. Biotechnol.

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Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

Cited by 14 publications

References 1 publication

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Multivariate analysis of combining ability for soybean resistance to Cercospora sojina Hara

Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

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