Efeitos do ágar no crescimento de explantes e na formação de calos em morfos pigmentares de Gracilaria domingensis (Kützing) Sonder ex Dickie (Gracilariales, Rhodophyta)

Ramlov, Fernanda; Plastino, Estela M.; Yokoya, Nair S.

doi:10.1590/s0100-84042009000300018

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…cell differentiation in the callus of Gracilaria vermiculophylla (Yokoya et al, 1999), G. chilensis (Collantes et al, 2004), G. domingensis (Ramlov, 2007), G. perplexa and G. tenuistipitata (Yokoya et al, 2004), and Gracilariopsis tenuifrons (Yokoya, 2000).…”

Section: Biotechnological Applicationsmentioning

confidence: 99%

Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

Yokoya¹,

Yoneshigue‐Valentin²

2011

Rev. bras. farmacogn.

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Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta. Micropropagation, or in vitro clonal propagation, allows the production of a large number of individuals within a short period. These micropropagated clones could be used as seedlings for seaweed cultivation, avoiding collection from natural beds. Consequently, there has been an increasing interest in micropropagation as a tool for preservation of populations of marine red algae on the Brazilian coast and for the sustainable production of raw material for commercial exploration. This paper reviews the literature on tissue culture and micropropagation of red algae published during the three last decades. Based on the literature, we can conclude that the regeneration process is complex and diversified in different species of Rhodophyta and that the success of micropropagation depends on this process. Species belonging to the orders Bangiales and Ceramiales showed low potential for regeneration, while Gigartinales species showed the highest potential for regeneration. Micropropagation of commercially important red algae is fundamental for the conservation of natural populations by providing seedlings for cultivation and for germplasm collections, both for the conservation of genetic diversity and for biotechnological applications.

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Section: Biotechnological Applicationsmentioning

confidence: 99%

Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

Yokoya¹,

Yoneshigue‐Valentin²

2011

Rev. bras. farmacogn.

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“…Thus, there is a wide range of protocols for decontamination (McCracken, 1989), using chemical treatments based on antibiotics or the potentially toxic germanium dioxide (Lewin, 1966;Markham & Haegmeier, 1982, Evans & Trewavas, 1991AguirreLipperheide et al, 1995;Kawai et al, 2005), together with disinfectant solutions such as detergents and sodium hypochlorite (Bravin et al, 2006;Ramlov et al, 2009). Protocols can include physical treatments such as ultrasound or brushing (Fries, 1963;Brown, 1982;McCracken, 1989).…”

Section: Introductionmentioning

confidence: 99%

Protocol for seaweed decontamination to isolate unialgal cultures

Fernandes¹,

Yokoya²,

Yoneshigue‐Valentin³

2011

Rev. bras. farmacogn.

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Decontamination protocols in seaweeds are essential tools for ecophysiological studies in laboratory cultures. These protocols consist of a set of procedures and physical and chemical treatments that must be adjusted for each species. Thus, the effects of explant size and of combinations of physical treatments (brushing and cutting) and chemical treatments (sodium hypochlorite, detergent, seawater, distilled water, germanium dioxide) on the process of obtaining unialgal culture of two pigmentar morphos of Hypnea musciformis were investigated. It was found that thallus segments 50 mm in length, when transported from the field to the laboratory, remained healthier and were less susceptible to epiphytes than those 7 mm in length. The collected material had surfaces contaminated by diatoms, which were weakly attached, as well as surface contamination caused by strongly attached Sahlingia subintegra. The most efficient combination of physical and chemical treatments was explant brushing, cutting and washing with detergent. This combination eliminated the contamination by S. subintegra, but not all of the diatom contamination. The population of the latter was reduced by using physical treatment and by washing with detergent and distilled water and then exterminated by using germanium dioxide (0.003 mg/L). Employing this protocol, unialgal cultures of H. musciformis could be established in approximately eight to ten weeks.

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The Gracilariaceae Germplasm Bank of the University of São Paulo, Brazil—a DNA barcoding approach

et al. 2012

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Efeitos do ágar no crescimento de explantes e na formação de calos em morfos pigmentares de Gracilaria domingensis (Kützing) Sonder ex Dickie (Gracilariales, Rhodophyta)

Cited by 6 publications

References 33 publications

Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

Protocol for seaweed decontamination to isolate unialgal cultures

The Gracilariaceae Germplasm Bank of the University of São Paulo, Brazil—a DNA barcoding approach

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