Phylogenetic analyses of Cladophora vagabunda (L.) C. Hoek (Cladophorales, Chlorophyta) from Brazil based on SSU rDNA sequences

Abstract

-(Phylogenetic analyses of Cladophora vagabunda (L.) C. Hoek (Cladophorales, Chlorophyta) from Brazil based on SSU rDNA sequences). The complete SSU rDNA was sequenced for 10 individuals of Cladophora vagabunda collected along the coast of Brazil. For C. rupestris (L.) Kütz. a partial SSU rDNA sequence (1634 bp) was obtained. Phylogenetic trees indicate that Cladophora is paraphyletic, but the section Glomeratae sensu lato including C. vagabunda from Brazil, Japan and France, C. albida (Nees) Kütz., C. sericea (Hudson) Kütz., and C. glomerata (L.) Kütz. is monophyletic. Within this group C. vagabunda is paraphyletic. The sequence identity for the SSU rDNA varied from 98.9% to 100% for the Brazilian C. vagabunda, and from 98.3% to 99.7% comparing the Brazilian individuals to the ones from France and Japan. Sequence identity of the Brazilian C. vagabunda to C. albida and C. sericea vary from 98.0% to 98.6%. The SSU rDNA phylogeny support partially the morphological characteristics presented by Brazilian populations of C. vagabunda. On the other hand, C. rupestris from Brazil does not group with C. rupestris from France, both sequences presenting only 96.9% of identity. The inclusion of sequences of individuals from Brazil reinforces the need of taxonomical revision for the genus Cladophora and for the complex C. vagabunda.Key words -Brazil, Cladophora vagabunda, Cladophorales, molecular phylogeny, SSU rDNA RESUMO -(Análises fi logenéticas de Cladophora vagabunda (L.) C. Hoek (Cladophorales, Chlorophyta) do Brasil, baseada nas seqüências SSU rDNA). A seqüência completa do SSU rDNA foi obtida para 10 indivíduos de Cladophora vagabunda coletadas ao longo da costa do Brasil. Uma seqüência parcial do SSU rDNA (1634 bp) foi obtida para C. rupestris (L.) Kütz. As árvores fi logenéticas indicam que Cladophora é parafi lético, mas a seção Glomeratae sensu lato compreendendo C. vagabunda do Brasil, Japão e França, C. albida (Nees) Kütz., C. sericea (Hudson) Kütz. e C. glomerata (L.) Kütz., é monofi lética. Dentro deste grupo, C. vagabunda é parafi lética. A identidade da seqüência para o SSU rDNA variou de 98,9% à 100% para C. vagabunda brasileira e de 98,3% a 99,7% quando comparados os indivíduos brasileiros aos da França e do Japão. A identidade da seqüência entre C. vagabunda brasileira e as duas outras espécies (C. albida e C. sericea) variou entre 98,0% e 98,6%. A fi logenia do SSU rDNA suporta parcialmente as caracteristícas morfológicas apresentadas pelas populações brasileiras de C. vagabunda. Por outro lado, C. rupestris do Brasil não se agrupou a C. rupestris da França, as duas seqüências apresentaram somente 96,9% de identidade. A inclusão de seqüências de indivíduos do Brasil reforçam a necessidade de uma revisão taxonômica para o gênero Cladophora e para o complexo C. vagabunda.

Molecular phylogeny of the Cladophoraceae (Cladophorales, Ulvophyceae), with the resurrection of Acrocladus Nägeli and Willeella Børgesen, and the description of Lurbica gen. nov. and Pseudorhizoclonium gen. nov.

“…The essentially marine species C. vagabunda penetrates into brackish waters with salinities as low as 15 psu. It had been shown in earlier studies that C. vagabunda contains several cryptic species (e.g., Bakker et al 1995, van den Hoek and Chihara 2000, Gestinari et al 2009, Hayakawa et al 2012. In our phylogenetic reconstructions, four lineages were grouped together with the freshwater clade (Fig.…”

The Genus Cladophora Kützing (Ulvophyceae) as a Globally Distributed Ecological Engineer

“…, Gestinari et al. ). Chromosome analyses reveal that Rhizoclonium , Chaetomorpha , and related Cladophora species share a basic chromosome number (six), chromosome morphology (three with median and three with submedian centromeres), and tendency to polyploidy (chromosome numbers of 12, 18, 24, etc.).…”

“…Recent molecular systematic data cluster some Cladophora species, such as the related and morphologically similar C. vagabunda (L.) van den Hoek, C. albida (Nees) K€ utz., C. sericea (Hudson) K€ utz., and C. glomerata, within the order Cladophorales sensu lato, along with several widespread genera with primarily unbranched filamentous morphology: Chaetomorpha (marine), Rhizoclonium (freshwater and marine), Pithophora (freshwater), and Basicladia (freshwater). Genetic sequence criteria cluster some other Cladophora species within Siphonocladales, a clade that includes diverse morphological types (Leliaert et al 2007, Gestinari et al 2009). Chromosome analyses reveal that Rhizoclonium, Chaetomorpha, and related Cladophora species share a basic chromosome number (six), chromosome morphology (three with median and three with submedian centromeres), and tendency to polyploidy (chromosome numbers of 12, 18, 24, etc.).…”

Ефективність Різних Методів Екстракції Днк, Придатної Для Плр Із Гербарних Зразків Водоростей Роду Cladophora Kütz.

“…Нині відомо про ряд філогенетичних досліджень, що базуються на аналізі послідовностей рибосомальних генів для видів Cladophora vagabunda (L. Hoek) та Cladophora albida (Nees) Kütz. (Cladophorales), а також видів із порядку Siphonocladales [3,5,11]. Подібних досліджень видів роду Cladophora, поширених в Україні, ще не проводили.…”