Anatomia foliar de Lolium multiflorum sensível e resistente ao glyphosate

Galvani, J.; Rizzardi,; Carneiro, Cerci Maria; Bianchi, Mário Antônio

doi:10.1590/s0100-83582012000200021

Cited by 12 publications

(9 citation statements)

References 10 publications

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“…Resistance Confirmation insularis to the herbicide also occurs due to differences in the translocation (Galvani et al, 2012).Also is possible that the thickness of leaves in this casehad no effect on the glyphosate differential sensitivity between biotypes, being the glyphosate tolerance major due by differential penetration or others translocation patterns (Monquero et al, 2004).…”

Section: Resultsmentioning

confidence: 85%

“…Most often, the increased presence of trichomes also reduces the amount of herbicide reaching the epidermis due to retaining droplets, but these factors are still not well understood (Procópio et al, 2003;Galvani et al, 2012;Marques et al, 2012).In terms of the presence of trichomes in both biotypes, it was observed that the development of D. insulariselevates the presence of these structures,which can be observed for both biotypes in both leaf surfaces, as was detected by Marques et al, (2012) and Sanyal et al (2006) in Brachiaria decumbens and in Echinochloa crus-galliand Setaria viridis (Table 2, Figure 4). In more advanced stages of leaf maturity, it was observed that the abaxial surface of the susceptible biotype presented more trichomes compared to the resistant biotypeas was reported in E.crus-galli( Table 2) (Sanyal et al, 2006).The abundance of trichomes in susceptible plants in the abaxial leaf surface may be related to the differential absorption (less than 16%) of the resistant plants in the first twelve hours compared to susceptible (Carvalho et al, 2012).…”

Section: Trichomesmentioning

confidence: 99%

“…Inryegrass (Lolium multiflorum) plants withleaf differences may contribute to glyphosate sensitivity (Galvani et al, 2012).Similarly, foliar absorption is one of the mechanisms to glyphosate resistance in Sorghum halepense, both by the leaf retention as slower absorption rate (Vila-Aiub et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Does Sourgrass leaf anatomy influence glyphosate resistance?

Barroso¹,

Galeano²,

Albrecht³

et al. 2015

Com. Sci.

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Sourgrass(Digitaria insularis, L. Mez ex Ekman)is a weed that requires high rates of glyphosate ((N-[phosphonomethyl]-glycine) forcontrol, verylittle of the herbicide applied isabsorbed by theleaves. Morphological and histological differences in leaves of glyphosate-resistant and glyphosate-susceptible plantsshould explain the contrast of variance between herbicide susceptibility. Leaves of different growth andphenological stages were collected and submitted to histological andelectron microscopy scanning analysis. Those plants were also submitted to a glyphosate dose-response curve analysis. The results suggest thatbiotypes exhibits differences infoliar structures that can influence the uptake and translocation of glyphosate. A thinparenchyma, lesser distance amongvascular bundles, and higher phloem sizewere found in resistant biotypes (differences close to 10%). Minor stomata number and higher epicuticular waxy deposition in stomata and leaf surface were found in resistant plants when leaves came from regrowth, leading to a possiblelesser herbicide absorption. Resistant plants showed absence of trichomes in late stages of development (64% less trichomes than susceptible plants). The higher concentration of trichomes in susceptible plants can be an absorption pathfor glyphosate getthrough the cuticle.Keywords: Absorption, herbicide resistance, parenchyma,trichomes A anatomia foliar de capim-amargoso influencia na resistência ao glyphosate? ResumoO capim-amargoso (Digitaria insularis, L. Mez ex Ekman) é uma plantadaninha que requer altas dosesde glifosato ((N-[fosfonometilo] glicina) para seu controle, sendo pouco do herbicida aplicado absorvido pelas folhas. Diferenças morfológicas e histológicas em folhas de biótipos resistentes e suscetíveis ao glifosato podem explicar a susceptibilidade diferencial ao herbicida. Folhas de diferentes estádios de crescimento de plantas foram coletadas e submetidas à análise histológica e de microscopia eletrônica de varredura. Essas plantas foram também submetidas a uma curva de dose-resposta ao glifosato. Os resultados sugerem diferenças foliares que podem influenciar a absorção e translocação de glyphosate. Um parênquima menos espesso, menores distâncias entre feixes vasculares, e maior tamanho de floema no biótipo resistente em relação ao biótipo suscetível foram observados (diferenças próximo a 10%). Menor número de estômatos e maior deposição de cera epicuticular em estômatos e superfície foliar nos biótipos resistentes foram encontradas quando a folha veio do rebrote, levando a uma possível menor absorção do herbicida. Em biótipos resistentes encontrou-se aausência de tricomas nos últimos estágios do desenvolvimento (64% a menos do que os tricomas plantas suscetíveis). A maior concentração de tricomas em plantas sensíveis, podem ser uma via de absorção para o glyphosate por entre a cutícula. Palavras-chave:Resistência a herbicidas, absorção, tricomas, parênquima

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Section: Resultsmentioning

confidence: 85%

Section: Trichomesmentioning

confidence: 99%

See 1 more Smart Citation

Does Sourgrass leaf anatomy influence glyphosate resistance?

Barroso¹,

Galeano²,

Albrecht³

et al. 2015

Com. Sci.

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“…The biotype of Lolium multiflorum resistant to glyphosate has shown less compacted mesophyll cells as well as a minor ratio of phloem in relation to xylem when compared to the susceptible one (Galvan et al, 2012).…”

Section: Leaf Anatomymentioning

confidence: 99%

Evaluation of the Mechanism Responsible for the Low-Level Resistance to Glyphosate in Goosegrass

et al. 2017

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-Goosegrass (Eleusine indica) infests various crops, being reported as low-level resistant (LLR) to glyphosate. The objectives of this study were to evaluate the mechanisms of resistance to glyphosate in goosegrass by the cyt-P450 complex to identify changes in leaf anatomy of these plants and quantify the accumulation of shikimate in biotypes susceptible and with LLR. The metabolic rate was assessed by applying the cyt-P450 malathion inhibitor 30 minutes before the herbicide application. The analysis of the leaf anatomy consisted of a characterization of anatomic cross-sections and evaluation of the effect of glyphosate on the leaf epidermis and the conducting vessels. Plant tissue was collected 24 hours after treatment (HAT) of the dose-response curve to evaluate the accumulation of shikimate. The LLR biotype has not shown glyphosate metabolism by the early application of malathion due to the control of about 65% with or without applying the inhibitor. There is no anatomical evidence to determine the differential absorption of glyphosate by LLR biotypes. However, there is evidence of lower absorption and translocation of glyphosate in this biotype by lower stomatal density and a smaller diameter of the phloem. However, the LLR biotype presents diameter of phloem of 0.027 mm and stomatal density of 91.23 mm -2 , values which are below the observed ones on the susceptible biotype. The susceptible biotype presents shikimate accumulation 17% higher compared to the biotype with LLR at a dose of 1,080 g e.a. ha -1 , allowing to infer that the resistance mechanism is related to the enzyme insensitivity to the herbicide, which enables the use of appropriate management practices to control this weed.Keywords: cyt-P450 monooxygenase, shikimate, absorption and translocation, EPSPs, Eleusine indica. RESUMO -O capim-pé-de-galinha (Eleusine indica) é infestante de diversos cultivos, sendo relatado como resistente de nível baixo (RNB) ao herbicida glyphosate. Os objetivos deste trabalho foram avaliar mecanismos de resistência de capim-pé-de

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“…(Italian ryegrass) and Sorghum halepense (L.) Pers. plants, leaf characteristics were responsible for differences in sensitivity to glyphosate, with less sensitive plants having lower glyphosate absorption (Galvani et al ., ). Another L. multiflorum population also showed tolerance to glyphosate due to lower translocation (Ghanizadeh et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Absorption and translocation of glyphosate in Spermacoce verticillata and alternative herbicide control

Fadin¹,

Tornisielo²,

Barroso

et al. 2018

Weed Research

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Glyphosate has been associated with control failures for Spermacoce verticillata in some Brazilian States. The objective of this work was to evaluate and determine the possible causes of this and propose alternative herbicides to use. Glyphosate was applied at three plant stages of development (2-4 leaves, 4-6 leaves and full bloom) where foliar absorption and translocation of glyphosate to various plants parts and time were analysed using the 14 C technique. Data were submitted to nonlinear regressions and analysis of variance, where means were compared by a Tukey test at 5% probability. Plant control by the application of different herbicides (19) in the same three stages of development of weed was evaluated. Twenty-one days after herbicide application, control was visually evaluated and data analysed and means were compared.Due to absorption and/or translocation problems, S. verticillata plants were not controlled by glyphosate. Plants with 4-6 leaves showed lower absorption and translocation of the herbicide to the leaf/root regions compared with plants at the beginning of their development. Plants at full bloom showed lower translocation of the herbicide to the root. In addition to the application of glyphosate at early stages of development, the application of paraquat, flumioxazin and mixtures of glyphosate with flumioxazin or cloransulam is recommended. Late applications could result in poor control, principally if glyphosate is used. Therefore, early applications are strongly recommended for control of this species.

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Anatomia foliar de Lolium multiflorum sensível e resistente ao glyphosate

Cited by 12 publications

References 10 publications

Does Sourgrass leaf anatomy influence glyphosate resistance?

Does Sourgrass leaf anatomy influence glyphosate resistance?

Evaluation of the Mechanism Responsible for the Low-Level Resistance to Glyphosate in Goosegrass

Absorption and translocation of glyphosate in Spermacoce verticillata and alternative herbicide control

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