Efficacy of a Kalanchoe gastonis-bonnieri extract to control bacterial biofilms and dental calculus in dogs

Abstract

RESUMO.-[Eficácia do extrato de Kalanchoe gastonis--bonnieri no controle do biofilme bacteriano e cálculo dentário em cães.] Um extrato aquoso de folhas da espé-cie medicinal Kalanchoe gastonis-bonnieri (aqui denominado como KGB) foi efetivo como um agente antimicrobiano contra as bactérias da cavidade oral de cães em testes in vitro. Neste estudo, investigou-se o efeito da administração oral tópica de KGB sobre o desenvolvimento do biofilme dental em cães da raça Beagle. Os experimentos foram realizados com um grupo experimental (0,2% de extrato de KGB), um grupo controle negativo (0,9% de solução salina) e um grupo controle positivo (0,12% de gluconato de clorexidina). Cada tratamento foi aplicado no interior da ca- An aqueous leaf extract of the medicinal species Kalanchoe gastonis-bonnieri (here denominated KGB) has been found to be effective as an antimicrobial agent against canine oral cavity bacteria in in vitro assays. In this study, we investigated the effect of topic oral administration of KGB on the development of dental biofilm in Beagle dogs. The experiments were performed with an experimental group (0.2% of KGB extract), a negative control group (0.9% of saline solution) and a positive control group (0.12% chlorhexidine). Each treatment was sprayed into the oral cavity daily for 28 days. Thirty Beagle dogs with similar characteristics and kept under the same management and diet were used. The measurement of dental plaque and calculus was performed using a computerized analytical method. The phenolic profile of KGB extract was analyzed by HPLC-DAD. KGB extract at 0.2% showed efficacy in controlling the formation of plaque compared to the negative control group, and dental calculus in relation to the negative and positive control groups. A significant difference was observed among these three groups. Peaks attributed to flavonoids and phenolic acids were identified in the HPLC-DAD chromatogram of the KGB extract. The presence of these substances could be related to the activity observed. Our findings demonstrate that treatment with KGB is effective in controlling periodontal disease in dogs, providing new insights into the medicinal properties of this plant. KGB extract has a potential use as a supplemental agent in pharmaceutical products for the prevention of periodontal disease. Efficacy of aINDEX TERMS: Kalanchoe gastonis-bonnieri, plant extract, bacterial biofilm, dental calculus, dogs, periodontal disease, dental plaque, flavonoids. TERMOS DE INDEXAÇÃO:Kalanchoe gastonis-bonnieri, extrato de plantas, biofilme bacteriano, cálculo dentário, cães, doença periodontal, cálculos dentários, placa bacteriana, flavonoides.

Prospección de extractos de plantas como complementos de la higiene bucal en perros Labrador Retriever

“…Assim, a progressão da formação do biofilme dentário induz diversas alterações no equilíbrio microbiano, podendo originar a placa dentária, formada por agregados de bactérias e seus subprodutos, componentes salivares, detritos orais e células epiteliais e inflamatórias ocasionais (Riggio et al, 2011;Whyte et al, 2014). A placa dentária, por sua vez, favorece o desenvolvimento da doença periodontal (PD), identificada como a ocorrência clínica mais comum em cães domésticos (Abdalla et al, 2017;Kyllar & Witter, 2005). A formação do biofilme ocorre quase imediatamente após um procedimento profissional de limpeza dos dentes.…”

Development and Evaluation of a Novel Oral Mucoadhesive Ointment Containing Pomegranate Peel Extract as an Adjuvant for Oral Hygiene of Dogs

“…1 The use of physical methods (such as regular tooth brushing, and provision of abrasive foods and toys) together with chemical methods (such as oral antiseptic-containing dentifrices) should be considered as a critical measure for microbial burden reduction and to control proliferation and establishment of these microbial colonies. [5][6][7][8] Solutions, creams, pastes, gels, and foams are the most commonly encountered pharmaceutical dosage forms for use in toothpastes or mouthwashes for the oral cavity. 1,7,8 However, the exposure time of the oral mucosa to the drugs in these products is short, whether due to the washing and rinsing process, or the presence of a continuous salivary flow and the mechanical movements of the mouth and tongue.…”

“…Compounds such as copaiba oil, clove essential oil, aloe extract, palm beachbells extract, guava extract, green tea extract, and pomegranate peel extract have been studied. 5,[15][16][17] According to Bolzan (2018), the natural oral microbiota of Labrador dogs was sensitive to various plant extracts, including the hydroalcoholic extracts of pomegranate peels that produced inhibition halos at all tested concentrations (5.0 to 250.0 mg/mL). 18 Ensuring proper dental homecare is one of the fundamental measures that owners can take for the maintenance of oral health in dogs.…”

“…1 The use of physical methods (such as regular tooth brushing, and provision of abrasive foods and toys) together with chemical methods (such as oral antiseptic-containing dentifrices) should be considered as a critical measure for microbial burden reduction and to control proliferation and establishment of these microbial colonies. 5 -8…”

Potential Therapeutic Effects of Underground Parts ofKalanchoe gastonis-bonnierion Benign Prostatic Hyperplasia

“…In a previous study we reported the isolation of the new flavonoid quercetin 3- O - α -rhamnopyranoside-7- O - β -D-glucopyranosyl-(1→3)- α -L-rhamnopyranoside, as well as vicenin-2, a C -glycosyl flavone, from the leaf extract of KGB [26]. We also demonstrated that aqueous extracts from K. gastonis-bonnieri (KGB) are effective in controling dental bacterial plaque and calculus in dogs [27].…”