Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

Abstract

Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp + /epf + /sly + genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed.

Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016

“…In Brazil, serotype 2 is described as the most prevalent, followed by serotypes 1 2 , 14, 7, and 9 [16][17][18][19]. The results of molecular serotyping in the present study corroborate the literature with a predominance of serotype 2/ 1 2 , which was identified in 86.0% (185/215) of strains from all sites and years of isolation.…”

Güney Çin’de Hastalıklı ve Sağlıklı Domuzlarda Streptococcus suis’in Antimikrobiyal Direnci, Serotipleri ve Genetik Çeşitliliği

“…These virulence genes are directly or indirectly involved in adhesion to host cells, survival in vivo, and immune escape [7] . By analyzing the main virulence genes of S. suis strains, it would be easier to understand the molecular characteristics and pathogenicity of the strains [8][9][10] .…”

“…İzolatlar, test edilen 42 antibiyotikten 22'sine %40'ın üzerinde ve 11'ine %80'in üzerinde değişen direnç gösterdi. Bu izolatlar 6 serotipe (2,3,7,9,19 ve 28) ait olup %9.9'u tiplendirilemeyen olarak karakterize edildi ve 8'i yeni (ST812-ST815, ST821-823 ve ST832) olmak üzere 15 sekans tipine (ST) sınıflandırıldı. Ayrıca, 81 suşta 6 adet virulans ile ilişkili gen şeması (VAGP) ve 10 adet rastgele amplifiye polimorfik DNA (RAPD) şeması bulundu.…”

>b<Análise comparativa de genomas de estirpes de >i<Streptococcus suis>/i< isoladas de suínos no Brasil>/b<

“…No Brasil os primeiros relatos de isolamento de animais doentes foram descritos nos anos de 1980 com diferentes quadros sistêmicos (REIS et al, 1980;FARINHA et al, 1981;BARCELLOS et al, 1984). Atualmente já foram identificados no Brasil diversos sorotipos, como o 1, 2,3,5,6,7,8,9,10,11,14, 18 e 22, causando diferentes quadros clínicos (MARTINEZ et al, 2003;COSTA et al, 2005;ROCHA et al, 2012;CALDERARO et al, 2016).…”

“…Com respeito a distribuição dos sorotipos no mundo, Wisselink et al (2000) reportaram dados de alguns países da Europa nos quais o sorotipo 2 é o mais prevalente seguido do 9 e 1, com frequência de 32%, 20% e 12%, respectivamente. Os dados atuais mostram que o sorotipo 2 é o mais isolado em todo o mundo chegando a representar frequências ainda maiores (HIGGINS;GOTTSCHALK, 2000;CALDERARO et al, 2016;KERDSIN et al, 2018). Entretanto, Estrada et al (2019) relataram a detecção de estirpes patogênicas dos sorotipos ½ e 7, nos Estados Unidos, com maior frequência do que o sorotipo 2.…”