Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens

Abstract

This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. A total of 864 samples were collected, among whom 248 originated from growing, 392 from rearing and 224 from production phase. Among the 864 samples, 2,8% where positives in bacteriologic technique and 15.3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41.7%), Salmonella Livingstone (33.3%), Salmonella Cerro (16.7%), Salmonella Senftenberg (4.2%) and Salmonella Schwarzengrund (4.2%). During growing phase Salmonella Livingstone was identified. These findings suggest vertical contamination in the group. During rearing and production phases, isolated materials belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in rearing phase is higher than in growing and production phases.

Identificação de Salmonella sp. em amostras de carcaças e ovos de frangos caipiras comercializados em feiras de Goiânia, Goiás.

“…Li et al (2019), identificaram 8,4% dos 369 isolados como Salmonella Corvallis, em carcaças de frangos comercializadas em feiras na China em 2016, utilizando bacteriologia convencional concluindo que o isolamento se deve às precárias condições sanitárias durante o processamento das carcaças; esta característica não pode ser observada, apenas deduzidas, pois as carcaças já se encontravam expostas ao comércio. Moraes et al (2016) isolaram Salmonella enterica em 2,8% das amostras analisadas de diversas origens como suabes de gaiolas, de bebedouros, de cloacas assim como em rações e insetos oriundos da produção de galinhas poedeiras comerciais utilizando a bacteriologia; dos isolados 16,7% foram de Salmonella Cerro, sugerindo a contaminação horizontal como uma importante forma de dispersão do patógeno (MORAES et al, 2016). Considerando a origem do isolado de Salmonella Cerro (carcaça), sugere-se provavelmente a mesma forma de contaminação (horizontal) das amostras obtidas uma vez que pode ter ocorrido a contaminação cruzado por falhas de higiene do manipulador.…”

7. Prevention and control of tick-borne anaplasmosis, cowdriosis and babesiosis in the cattle industry

“…The direct contact between these insects and the litter as well as their feeding on dead and sick broiler chickens make A. diaperinus effective reservoirs of many avian disease agents and parasites, including some food-borne diseases (Axtell and Arends 1990;Chernaki-Leffer et al 2010;Moraes et al 2016;Watson et al 2000). Because of this ability to transmit pathogens that cause disease, a large population of lesser mealworms poses a significant threat to the production of safe chicken meat.…”

1. Livestock pests and vector-borne diseases – a much neglected subject

“…The direct contact between these insects and the litter as well as their feeding on dead and sick broiler chickens make A. diaperinus effective reservoirs of many avian disease agents and parasites, including some food-borne diseases (Axtell and Arends 1990;Chernaki-Leffer et al 2010;Moraes et al 2016;Watson et al 2000). Because of this ability to transmit pathogens that cause disease, a large population of lesser mealworms poses a significant threat to the production of safe chicken meat.…”