Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação

Abstract

The objective of this study was to detect Brucella abortus in lymph nodes of buffaloes as well as to describe the lesions caused. Nineteen buffalo cows in various stages of pregnancy, serologically positive for brucellosis and subjected to culling were used. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. Fragments of lymph nodes were collected for histopathology and qPCR. The detection of B. abortus DNA in the lymph nodes was checked from the fourth month of pregnancy in seven buffaloes and in a post-calving buffalo. Acute to chronic lymphadenitis was histologically diagnosed. B. abortus DNA was detected in all evaluated groups of lymph nodes; the mammary lymph nodes were the most affected.

Brucellosis in water buffaloes

“…Chronic suppurative endometritis with areas of fibrosis and the presence of numerous basophilic coccoid bacterial colonies on the endometrial ulcerated surface is also observed. In the lymph nodes, congestion, edema, fibrin, infiltration by neutrophils, histiocytes and plasma cells are observed, which characterize regional lymphadenitis (Sousa et al 2015b). In buffalo fetuses, liver with small foci of lymphocytic infiltrates distributed throughout the parenchyma, with mild to moderate injury, diffuse vacuolization of hepatocytes, kidney and spleen congestion, congestion, edema and fibrin in the lymph nodes are detected (Sousa et al 2015a).…”

“…Sousa 2 , Felipe M. Salvarani 2 , Henrique A. Bomjardim 2 , Marilene F. Brito 3 and José D. Barbosa 2 * with fibrinous suppurative necrotic placentitis (Sousa et al 2015a). Brucellosis has been diagnosed in water buffaloes from Egypt (Gentile 1957, Menshawy et al 2014, Italy (Guarino et al 2001), Trinidad and Tobago (Fosgate et al 2002), Pakistan (Nasir et al 2004), Argentina (Konrad et al 2013) and Brazil (Ogassawara et al 1969, Santa Rosa et al 1969, Láu & Singh 1985, Bastianetto et al 2005, Silva et al 2014a, Sousa et al 2015a, 2015b.…”

“…The infection starts mainly in the oral, nasopharyngeal and conjunctival mucosa or in broken skin. After contamination, the bacteria are phagocytosed primarily by macrophages and transported to regional lymph nodes, where, as facultative intracellular microorganisms, they multiply inside the cells, particularly cells of the mononuclear phagocyte system, and may evade the immune response for weeks to months, causing inflammatory and anatomopathological changes, such as hyperplasia and lymphadenitis (Carvalho Neta et al 2010, Sousa et al 2015b.…”

“…From the regional lymph nodes, the bacteria can disseminate intracellularly in macrophages or extracellularly, through the hematogenous or lymphatic routes, spreading to other lymph nodes, especially in the mammary glands and organs rich in phagocytic mononuclear cells (Harmon et al 1988, Xavier et al 2009, Sousa et al 2015b. The predilection sites of the Brucella genus are those that offer substances necessary to their metabolism, such as erythritol, present in the pregnant uterus, mammary and osteoarticular tissues and the organs of the male reproductive system.…”

Emerging Infectious Diseases in Water Buffalo: An Economic and Public Health Concern

“…In addition, developed countries in Latin America, Southern Europe, Africa, South and Southeast Asia, and the Middle East, especially in the rural areas, where livestock rearing and production of dairy products and by-products is crucial for family income [2,7,56,[75][76][77][78][79][80].…”