Diagnóstico de paratuberculose por biópsia retal em búfalos

Abstract

Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal biopsies with possible lesions in mesenteric nodes and the intestine. The history of the herd and clinical examination revealed progressive weight loss and non-responsive antimicrobial diarrhea, dehydration and submandibular edema. Rectal biopsies showed in six buffaloes microscopically suggestive lesions for paratuberculosis through hematoxilin-eosin staining (HE), characterized by moderate multifocal granulomatous enteritis with epithelioid cell infiltration. In four buffaloes Langhans giant cells were found. In 15 buffaloes lymphocytic infiltrate was observed in the lamina propria of the large intestine. Ziehl-Neelsen staining (ZN) revealed in 4.3% (6/140) acid-fast bacilli in the rectal mucosa. Real time PCR amplified to 5.71% (7/140) Mycobacterium avium subsp. paratuberculosis (Map) DNA. 11 buffalos were submitted to postmortem examination, gross examination revealed augmented mesenteric nodes with whitish areas in the cut surface. The mucosa of the small intestine was irregular and thickened, with evident traverse folds and Peyer plates. The brownish intestinal content was fluid, the ileocecal valve area thickened and edematous with evident lymphatic vessels. Histological lesions in the mesenteric lymph node and small intestine four buffalo were compatible with those already described in the literature, and presented acid-fast bacilli by ZN staining and amplification of Map genetic material in qPCR. The concordance between the rectal biopsy and the postmortem samples was in agreement with the Kappa test (K=0.792) and was considered substantial or high. The rectal biopsy showed to be promising and can be used by practitioners, together with other techniques, for antemortem diagnosis in buffalo herds suspected to be affected by paratuberculosis. Samples obtained by rectal biopsy were suitable for ZN staining and real time PCR. Rectal biopsy can be used as a tool for diagnosis and control of Johne´s disease in buffaloes to select and eliminate positive animals within the herd and decrease gradually the spread of Map.

Use of Environmental and Genetic Descriptors to Integrate Herds of Sheep

“…Em seguida, outros relatos surgiram nos estados do Maranhão e Alagoas (REIS et al, 2015;BRITO et al, 2016;MORAES PEREIRA et al, 2020 A relação do consumo de alimentos de origem animal contaminados com MAP e o desenvolvimento da doença de Chron nos humanos ainda não está devidamente esclarecida (WADDELL et al, 2016).…”

Passado, Presente E Futuro Na Pesquisa De Coccidiose Aviária

“…Em seguida, outros relatos surgiram nos estados do Maranhão e Alagoas (REIS et al, 2015;BRITO et al, 2016;MORAES PEREIRA et al, 2020 A relação do consumo de alimentos de origem animal contaminados com MAP e o desenvolvimento da doença de Chron nos humanos ainda não está devidamente esclarecida (WADDELL et al, 2016).…”

Teores de cobre, zinco e ferro no fígado de búfalos (Bubalus bubalis) com paratuberculose

“…O estudo foi conduzido em duas propriedades de criação de bubalinos localizadas nos municípios de São Mateus e São Luís, região nordeste do estado do Maranhão, onde já existiam casos de paratuberculose (Belo- Reis et al 2015). Os históricos foram coletados junto aos proprietários, durante as visitas.…”

“…Em todos os animais foi realizada biópsia retal de acordo com metodologia descrita por Belo- Reis et al (2015), para pesquisa de Map através de PCR em tempo real (qPCR) e Ziehl-Neelsen (ZN). Sete animais foram positivos para presença de Map e alocados no Grupo1, e os outros seis foram negativos e alocados no Grupo 2.…”